The bimodular G57-V577 polypeptide chain of the class B penicillin- binding protein 3 of Escherichia coli catalyzes peptide bond formation from thiolesters and does not catalyze glycan chain polymerization from the lipid II intermediate

Journal of Bacteriology

Volumn 179, Issue 19, 1997, Pages 6005-6009

  Adam, Maggy ^a   Fraipont, Claudine ^a   Rhazi, Nourredine ^a   Nguyen Distèche, Martine ^a   Lakaye, Bernard ^a   Frère, Jean Marie ^a   Devreese, Bart ^b   Van Beeumen, Jozef ^b   Van Heijenoort, Yvelyne ^c   Van Heijenoort, Jean ^c   Ghuysen, Jean Marie ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BETA LACTAM ANTIBIOTIC; CEPHALOSPORIN DERIVATIVE; DISACCHARIDE; GAMMA GLUTAMYLTRANSFERASE; GLYCAN; GLYCOSYLTRANSFERASE; PENICILLIN BINDING PROTEIN; PENICILLIN DERIVATIVE; THIOESTER; THIOL ESTER HYDROLASE;

ACYLATION; ARTICLE; BINDING KINETICS; CATALYSIS; CONTROLLED STUDY; DRUG PROTEIN BINDING; ENZYME ACTIVITY; ESCHERICHIA COLI; GLYCOSYLATION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0030921172     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.19.6005-6009.1997     Document Type: Article

References (30)

1. Adam, M., C. Damblon, B. Plaitin, L. Christiaens, and J. M. Frère. 1990. Chromogenic depsipeptide substrates for β-lactamases and penicillin-sensitive DD-peptidases. Biochem. J. 270:525-529.
2. Adam, M., C. Damblon, M. Jamin, W. Zorzi, V. Dusart, M. Galleni, A. El Kharroubi, G. Piras, B. G. Spratt, W. Keck, J. Coyette, J. M. Ghuysen, Nguyen-Distèche, M. and J. M. Frère. 1991. Acyltransferase activities of the high-molecular-mass essential penicillin-binding proteins. Biochem. J. 279: 601-604.
3. Begg, K. J., A. Takasuga, D. H. Edwards, S. J. Dewar, B. G. Spratt, H. Adachi, T. Ohta, H. Matsuzawa, and W. D. Donachie. 1990. The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide. J. Bacteriol. 172:6697-6703.
4. Cartwright, S. J., and A. L. Fink. 1982. Isolation of a covalent intermediate in β-lactamase I catalysis. FEBS Lett. 137:186-188.
5. Curtis, N. A. C., D. Orr, G. W. Ross, and M. G. Boulton. 1979. Affinities of penicillins and cephalosporins for the penicillin-binding proteins of Escherichia coli K-12 and their antibacterial activity. Antimicrob. Agents Chemother. 16:533-539.
6. De Meester, F., B. Joris, G. Reckinger, C. Bellefroid-Bourguignon, J. M. Frère, and S. G. Waley. 1987. Automated analysis of enzyme inactivation phenomena. Application to β-lactamases and DD-peptidases. Biochem. Pharmacol. 36:2393-2403.
7. Englebert, S., G. Piras, A. El Kharroubi, B. Joris, J. Coyette, M. Nguyen-Distèche and J. M. Ghuysen. 1993. Modular design of the bi(multi?)functional penicillin-binding proteins, p. 319-333. In M. A. de Pedro, J. V. Höltje, and W. Löffelhardt (ed.), Bacterial growth and lysis: metabolism and structure of the bacterial sacculus. Plenum Publishing Co., New York, N.Y.
8. Fenn, J. B., M. Mann, C. K. Meng, S. F. Wong, and C. M. Whitehouse. 1989. Electrospray ionisation for mass spectrometry of large biomolecules. Science 246:64-71.
9. Fraipont, C., M. Adam, M. Nguyen-Distèche, W. Keck, J. Van Beeumen, J. Ayala, B. Granier, H. Hara, and J. M. Ghuysen. 1994. Engineering and overexpression of periplasmic forms of the penicillin-binding protein 3 of Escherichia coli. Biochem. J. 298:189-195.
10. Frère, J. M., M. Nguyen-Distèche, J. Coyette, and B. Joris. 1992. Mode of action: interaction with the penicillin-binding proteins, p. 148-195. In M. I. Page (ed.), The chemistry of β-lactams. Chapman and Hall, Glasgow, Scotland.
11. Georgopapadakou, N. H., S. A. Smith, and R. B. Sykes. 1982. Mode of action of azthreonam. Antimicrob. Agents Chemother. 21:950-956.
12. Ghuysen, J. M. 1991. Serine β-lactamases and penicillin-binding proteins. Annu. Rev. Microbiol. 45:37-67.
13. Ghuysen, J. M., P. Charlier, J. Coyette, C. Duez, E. Fonzé, C. Fraipont, C. Goffin, B. Joris, and M. Nguyen-Distèche. 1996. Penicillin and beyond: evolution, protein fold, multimodular polypeptides and multiprotein complexes. Microb. Drug Resist. 2:163-175.
14. Ghuysen, J. M., J. M. Frère, M. Leyh-Bouille, M. Nguyen-Distèche, and J. Coyette. 1986. Active-site serine D-alanyl-D-alanine-cleaving peptidase-catalysed acyl transfer reactions. Procedures for studying the penicillin-binding proteins of bacterial plasma membranes. Biochem. J. 235:159-165.
15. Ghuysen, J. M., M. Leyh-Bouille, J. N. Campbell, R. Moreno, J. M. Frère, C. Duez, M. Nieto, and H. R. Perkins. 1973. Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase-transpeptidase for peptide acceptors. Biochemistry 12:1243-1251.
16. Goffin, C., C. Fraipont, J. Ayala, M. Terrak, M. Nguyen-Distèche, and J. M. Ghuysen. 1996. The non-penicillin-binding module of the tripartite penicillin-binding protein 3 of Escherichia coli is required for folding and/or stability of the penicillin-binding module and the membrane-anchoring module confers cell septation activity on the folded structure. J. Bacteriol. 178:5402-5409.
17. Grandchamps, J., M. Nguyen-Distèche, C. Damblon, J. M. Frère, and J. M. Ghuysen. 1995. The Streptomyces K15 active-site serine DD-transpeptidase. Specificity profile for peptide, thiolester and ester carbonyl donors and pathways of the transfer reactions. Biochem. J. 307:335-339.
18. Höltje, J. V. 1996. Molecular interplay of murein synthetases and murein hydrolases in Escherichia coli. Microb. Drug Resist. 2:99-103.
19. Ishino, F., and M. Matsuhashi. 1981. Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence. Biochem. Biophys. Res. Commun. 101: 905-911.
20. Jamin, M., M. Adam, C. Damblon, L. Christiaens, and J. M. Frère. 1991. Accumulation of acyl-enzyme in DD-peptidase-catalysed reactions with analogues of peptide substrates. Biochem. J. 280:499-506.
21. Jamin, M., C. Damblon, S. Millier, R. Hakenbeck, and J. M. Frère. 1993. Penicillin-binding protein 2x of Streptococcus pneumoniae: enzymic activities and interactions with β-lactams. Biochem. J. 292:735-741.
22. Jamin, M., J. M. Wilkin, and J. M. Frère. 1993. A new kinetic mechanism for the concomitant hydrolysis and transfer reactions catalysed by bacterial DD-peptidases. Biochemistry 32:7278-7285.
23. Lakaye, B., C. Damblon, M. Jamin, M. Galleni, S. Lepage, B. Joris, J. Marchand-Brynaert, C. Frydrych, and J. M. Frère. 1994. Synthesis, purification and kinetic properties of fluorescein-labelled penicillins. Biochem. J. 300:141-145.
24. Lutkenhaus, J. 1993. Fts ring in bacterial cytokinesis. Mol. Microbiol. 9:403-409.
25. Martin, M. T., and S. G. Waley. 1988. Kinetic characterization of the acyl-enzyme mechanism for β-lactamase I. Biochem. J. 254:923-925.
26. Nguyen-Distèche, M., M. Leyh-Bouille, S. Pirlot, J. M. Frère, and J. M. Ghuysen. 1986. Streptomyces K15 uo-peptidase-catalysed reactions with ester and amide carbonyl donors. Biochem. J. 235:167-176.
27. Pisabarro, A. G., R. Prats, D. Vazquez, and A. Rodriguez-Tébar. 1986 Activity of penicillin-binding protein 3 from Escherichia coll. J. Bacteriol. 168:199-206.
28. Spratt, B. G., J. Zhou, M. Taylor, and M. J. Merrick. 1996. Monofunctional biosynthetic peptidoglycan transglycosylases. Mol. Microbiol. 19:639-647.
29. van Heijenoort, Y., M. Gómez, M. Derrien, J. Ayala, and J. van Heijenoort. 1992. Membrane intermediates in the peptidoglycan metabolism of Escherichia coli: possible roles of PBP 1b and PBP 3. J. Bacteriol. 174:3549-3557.
30. Vicente, M., and J. Errington. 1996. Structure, function and controls in microbial division. Mol. Microbiol. 20:1-7.