The modE gene product mediates molybdenum-dependent expression of genes for the high-affinity molybdate transporter and modG in Azotobacter vinelandii

Microbiology

Volumn 142, Issue 8, 1996, Pages 1997-2004

  Mouncey, Nigel J ^a,c   Mitchenall, Lesley A ^a,b   Pau, Richard N ^a,b  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b JOHN INNES CENTRE   (United Kingdom)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Molybdate; Molybdenum; Nitrogenase; Regulation and transport; Vanadium

Indexed keywords

CARRIER PROTEIN; GENE PRODUCT; MOLYBDENUM; MOLYBDIC ACID; TUNGSTEN DERIVATIVE; VANADIC ACID;

ARTICLE; AZOTOBACTER VINELANDII; BACTERIAL GENE; BACTERIAL METABOLISM; BACTERIUM MUTANT; CONTROLLED STUDY; GENE REPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS);

EID: 0029824740     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-142-8-1997     Document Type: Article

References (46)

1. Baldock, C. P., Pau, R. N., Barnes, P. & Lutkin, J. E. (1994). Production of molybdenum from radionuclide generators for the investigation of genes associated with molybdenum transporters. Nucl Med Commun 15, 264.
2. Benemann, J. R., Smith, G. M., Kostel, P. J. & McKenna, C. E. (1973). Tungsten incorporation into Azotobacter vinelandii nitrogenase. FEBS Lett 29, 219-221.
3. Bishop, P. E. & Brill, W. J. (1977). Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen. J Bacteriol 130, 954-956.
4. Bowman, B. J. (1983). Vanadate uptake in Neurospora crassa occurs via phosphate transport system II. J Bacteriol 153, 286-291.
5. Chauret, C., Barraquio, W. L. & Knowles, R. (1992). Molybdenum incorporation in denitrifying Azospirillum brasilense Sp7. Can J Microbiol 38, 1042-1047.
6. Cox, G. B., Webb, D. & Rosenberg, H. (1989). Specific amino acid residues in both the PstB and PstC proteins are required for phosphate transport by the Escherichia coli Pst system. J Bacteriol 171, 1531-1534.
7. Dean, D. R., Bolin, J. T. & Zheng, L. (1993). Nitrogenase metallo-clusters: structures, organization and synthesis. J Bacteriol 175, 6737-6744.
8. Eady, R. R. (1995). The enzymology of biological nitrogen fixation. Sci Prog 78, 1-17.
9. Elliott, B. K. & Mortenson, L. E. (1975). Transport of molybdate by Clostridium pasteurianum. J Bacteriol 124, 1295-1301.
10. Fleishmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavge, A. R., Bult C. J., Tomb, J. F., Dougherty, B. A., Merrick, J. M., McKenney, K., Sutton, G., Fitzhugh, W., Fields, C., Gocayne, J. D., Scott, J., Shirley, R., Liu, L. I., Glodek, A., Kelley, J. M., Weidman, J. F., Phillips, C. A., Spriggs, T., Hedblom, E., Cotton, M. D., Utternack, T. R., Hanna, M. C., Nguyen, D. T., Saudek, D. M., Brandon, R. C., Fine, L. D., Fritchman, J. L., Fuhrmann, J. L., Geoghagen, N. S. M., Gnehm, C. L., McDonald, L. A., Small, K. V., Fraser, C. M., Smith, H. O. & Venter, J. C. (1995). Whole-genome random sequencing and assembly of Haemophilus influenzae. Science 269, 496-512.
11. Hales, B. J. & Case, E. E. (1987). Nitrogen fixation by Azotobacter vinelandii in tungsten-containing medium. J Biol Chem 262, 16205-16211.
12. Hemschemeier, S., Grund, M., Keuntje, B. & Eichenlaub, R. (1991). Isolation of Escherichia coli mutants defective in the uptake of molybdate. J Bacteriol 173, 6499-6506.
13. Hryniewicz, M., Sirko, A., Palucha, A., Bock, A. & Hulanicka, D. (1990). Sulfate and thiosulfate transport in Escherichia coli K-12: identification of a gene encoding a novel protein involved in thiosulfate binding. J Bacteriol 172, 3358-3366.
14. Jacobitz, S. & Bishop, P. E. (1992). Regulation of nitrogenase-2 in Azotobacter vinelandii by ammonium, molybdenum, and vanadium. J Bacteriol 174, 3884-3888.
15. Jacobson, M. R., Premakumar, R. & Bishop, P. E. (1986). Transcriptional regulation of nitrogen fixation by molybdenum in Azotobacter vinelandii. J Bacteriol 167, 480-486.
16. Ji, G. & Silver, S. (1992). Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of the Staphylococcus aureus plasmid pI258. Proc Natl Acad Sci USA 89, 9474-9478.
17. Joerger, R. D., Jacobson, M. R., Premakumar, R., Wolfinger, E. D. & Bishop, P. E. (1989). Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii. J Bacteriol 171, 1075-1086.
18. Joerger, R. D., Loveless, T. M., Pau, R. N., Mitchenall, L. A., Simon, B. H. & Bishop, P. J. (1990). Nucleotide sequences and mutational analysis of the structural genes for nitrogenase 2 of Azotobacter vinelandii. J Bacteriol 172, 3400-3408.
19. Kim, J. & Rees, D. C. (1992). Crystallography structure and functional implications of the nitrogenase molybdenum-iron protein from Azotobacter vinelandii. Nature 360, 553-560.
20. Laudenbach, D. E. & Grossman, A. R. (1991). Characterisation and mutagenesis of sulphur-regulated genes in a cyanobacterium: evidence for function in sulphate transport. J Bacteriol 173, 2739-2750.
21. Lee, J. H., Wendt, J. C. & Shanmugam, K. T. (1990). Identification of a new gene, molR, essential for utilization of molybdate by Escherichia coli. J Bacteriol 172, 2079-2087.
22. Luque, F. & Pau, R. N. (1991). Transcriptional regulation by metals of structural genes for Azotobacter vinelandii nitrogenases. Mol Gen Genet 227, 481-487.
23. Luque, F., Mitchenall, L. A., Chapman, M., Christine, R. & Pau, R. N. (1993). Characterization of genes involved in molybdenum transport in Azotobacter vinelandii. Mol Microbiol 7, 447-459.
24. Maupin-Furlow, J. A., Rosentel, J. K., Lee, J. H., Deppenmeier, U., Gunsalus, R. P. & Shanmugam, K. T. (1995). Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli. J Bacteriol 177, 4851-4856.
25. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
26. Miller, J., Scott, D. & Amy, N. (1987). Molybdenum-sensitive transcriptional regulation of the cblD locus of Escherichia coli. J Bacteriol 169, 1853-1860.
27. Mouncey, N. J., Mitchenall, L. A. & Pau, R. N. (1995). Mutational analysis of genes of the mod locus involved in molybdenum transport, homeostasis and processing in Azotobacter vinelandii. J Bacteriol 177, 5294-5302.
28. Page, W. J. & von Tigerstrom, M. (1979). Optimal conditions for the transformation of Azotobacter vinelandii. J Bacteriol 139, 1058-1061.
29. Pau, R. N., Mitchenall, L. A. & Robson, R. L. (1989). Genetic evidence for an Azotobacter vinelandii nitrogenase lacking molybdenum and vanadium. J Bacteriol 171, 124-129.
30. Pienkos, P. T. & Brill, W. J. (1981). Molybdenum accumulation and storage in Klebsiella pneumoniae and Azotobacter vinelandii. J Bacteriol 145, 743-751.
31. Premakumar, R., Jacobitz, S., Ricke, S. C. & Bishop, P. E. (1996). Phenotypic characterisation of a tungsten-tolerant mutant of Azotobacter vinelandii. J Bacteriol 178, 691-696.
32. Rech, S., Deppenmeier, U. & Gunsalus, R. P. (1995). Regulation of the molybdate transport operon, modABCD, of Escherichia coli in response to molybdate availability. J Bacteriol 177, 1023-1029.
33. Robson, R. L., Eady, R. R., Richardson, T. H., Miller, R. W., Hawkins, M. & Postgate, J. R. (1986). The alternative nitrogenase of Azotobacter chroococcum is a vanadium enzyme. Nature 322, 388-390.
34. Rosen, B. P., Shi, W. & Wu, J. (1995). Regulation of the arsenical resistance operon, J Cell Biochem S1A SIA, 240.
35. Rosentel, J. K., Healy, F., Maupin-Furlow, J. A., Lee, J. H. & Shanmugam, K. T. (1995). Molybdate and regulation of mod (molybdate transport), fdhF, and hyc (formate hydrogenlyase) operons in Escherichia coli. J Bacteriol 177, 4857-4864.
36. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1987). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
37. Steed, P. M. & Wanner, B. L. (1993). Use of the rep technique for allele replacement to construct mutants with deletions of the pstSCAB-phoU operon: evidence of a new role for the PhoU protein in the phosphate regulon. J Bacteriol 175, 6797-6809.
38. Stewart, V. (1993). Nitrate regulation of anaerobic respiratory gene expression in Escherichia coli. Mol Microbiol 9, 425-434.
39. Stiefel, E. I. (1993). Molybdenum enzymes, cofactors and chemistry. In Molybdenum Enzymes, Cofactors and Model Systems. ACS Symposium Series 535, pp. 1-18. Edited by E. I. Stiefel, D. Coucouvanis & W. E. Newton. Washington, DC: American Chemical Society.
40. Surin, B. P., Rosenberg, H. & Cox, G. B. (1985). Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships. J Bacteriol 161, 189-198.
41. Walkenhorst, H. M., Hemschemeier, S. K. & Eichenlaub, R. (1995). Molecular analysis of the molybdate uptake operon, modABCD, of Escherichia coli and modR, a regulatory gene. Microbiol Res 150, 347-361.
42. Walmsley, J., Toukdarian, A. & Kennedy, C. (1994). The role of regulatory genes nifA, vnfA, anfA, nfrX, ntrC and rpoN in expression of genes encoding the three nitrogenases of Azotobacter vinelandii. Arch Microbiol 162, 422-429.
43. Wang, G., Angermüller, S. & Klipp, W. (1993). Characterization of Rhodobacter capsulatus genes encoding a molybdenum transport system and putative molybdenum-pterin-binding proteins. J Bacteriol 175, 3031-3042.
44. Wanner, B. L. (1993). Gene regulation by phosphate in enteric bacteria. J Cell Biochem 51, 47-54.
45. Willsky, G. R. (1990). Vanadium in the biosphere. In Vanadium in Biological System: Physiology and Biology, pp. 1-24. Edited by N. D. Chasteen. Dordrecht: Kluwer Academic Publishers.
46. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.