Quantitation of hydroxyl radicals produced by radiation and copper- linked oxidation of ascorbate by 2-deoxy-d-ribose method

Free Radical Biology and Medicine

Volumn 22, Issue 7, 1997, Pages 1129-1138

  Biaglow, John E ^a   Manevich, Yefim ^a   Uckun, Fatih ^c   Held, Kathryn D ^b  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c UNIVERSITY OF MINNESOTA   (United States)

Author keywords

2 Deoxy D ribose; Ascorbate; Copper; Dithiothreitol; Fluorescence; Hydroxyl radical; Malondialdehyde; Oxygen consumption; Radiation

Indexed keywords

2 DEOXYRIBOSE; ASCORBIC ACID; COPPER ION; DITHIOTHREITOL; HYDROGEN PEROXIDE; HYDROXYL RADICAL; OXYGEN; THIOBARBITURIC ACID REACTIVE SUBSTANCE;

ARTICLE; FLUORESCENCE; GAMMA IRRADIATION; IONIZING RADIATION; OXIDATION; OXIDATIVE STRESS; OXYGEN CONSUMPTION; PRIORITY JOURNAL; REPRODUCIBILITY; SIGNAL TRANSDUCTION;

ASCORBIC ACID; COPPER; DEOXYRIBOSE; DITHIOTHREITOL; FREE RADICALS; GAMMA RAYS; HYDROGEN-ION CONCENTRATION; HYDROXYL RADICAL; KINETICS; OXIDATION-REDUCTION; OXYGEN; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; TEMPERATURE; THIOBARBITURIC ACID REACTIVE SUBSTANCES;

EID: 0030901059     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(96)00527-8     Document Type: Article

References (39)

1. Mee L. K., Adelstein S. J. Radiation damage to histone H2A by the primary aqueous radicals. Radiat. Res. 110:1987;155-160.
2. Dizdaroglu M., Gajewski E. Structure and mechanism of hydroxyl radical induced formation of a DNA-protein crosslink involving thymine and lysine in nucleohistone. Cancer Res. 49:1989;3463-3467.
3. Schuessler H., Schilling K. Oxygen effect in the radiolysis of proteins. Part 2. Bovine serum albumin. Int. J. Radiat. Biol. 45:1984;267-281.
4. Ukun, F. M.; Biaglow, J. E. Protooncogene family protein tryosine kinase p53/p56 LYN contributes to radiation resistance of human B-cell precursor leukemias by triggering a delayed cell cycle progression at the G2-M transition check point.Cancer Res. (in press).
5. Biaglow J. E., Held K. D., Manevich Y., Tuttle S., Kachur A., Uckun F. Role of guanosine triphosphate in ferric ion linked Fenton chemistry. Radiat. Res. 145:1996;554-562.
6. Biaglow J. E., Clark E. P., Epp E. R., Morse-Guadio M., Varnes M., Mitchell J. B. Nonprotein thiols and the radiation response of A549 human lung carcinoma cells. Int. J. Radiat. Biol. 44:1983;489-495.
7. Halliwell B., Grootveld M., Gutteridge B. Methods for the measurement of hydroxyl radicals in biochemical systems: Deoxyribose degradation and aromatic hydroxylation. Methods Biochem. Anal. 33:1987;59-87.
8. Buettner G. R., Jurkiewicz B. A. Catalytic metals, ascorbate and free radicals: Combinations to avoid. Radiat. Res. 145:1996;532-541.
9. Burkett M. J., Gilbert B. C. Model studies of the iron-catalyzed Haber-Weis cycle and the ascorbate driven Fenton reaction. Free Radic. Res. Commun. 20:1990;265-280.
10. Biaglow, J. E.; Kachur, A.; Manevich, Y. Quantitation of iron-linked hydroxylation reactions.Free Radic. Biol. Med. (in press).
11. Koch C. J., Howell R. L., Biaglow J. E. Ascorbate anion potentiates cytotoxicity of nitro-aromatic compounds under hypoxic and anoxic conditions. Br. J. Cancer. 39:1979;321-329.
12. Biaglow J. E., Jacobson B., Koch C. The catalytic effect of the carcinogen "4-nitroquinoline-N-oxide" on the oxidation of vitamin C. Biochem. Biophys. Res. Commun. 70:1976;1316-1323.
13. Biaglow J. E., Jacobson B., Varnes M., Koch C. The oxidation of ascorbate by electron affinic drugs and carcinogens. Photochem. Photobiol. 28:1978;869-876.
14. Biaglow J. E., Jacobson B. The reaction of hypoxic cell radiosensitizing drugs with Vitamin C. Br. J. Cancer. 50:1977;844-845.
15. Kadiiska M. D., Hanna P. M., Hernandez L., Mason R. P. In vivo evidence of hydroxyl radical formation after acute copper and ascorbic acid intake: Electron spin resonance spin-trapping investigation. Mol Pharmacol. 42:1992;723-729.
16. Held K. D., Bren G. D., Melder D. C. Interactions of radioprotectors and oxygen in cultured mammalian cells. II. Effects of dithiothreitol on radiation-induced DNA damage and comparison with cell survival. Radiat. Res. 110:1986;296-306.
17. Held K. D., Melder D. C. Toxicity of the sulfhydryl-containing radioprotector dithiothreitol. Radiat. Res. 112:1987;544-554.
18. Held K. D., Biaglow J. E. Role of copper in the oxygen radical-mediated toxicity of the thiol-containing radioprotector dithiothreitol in mammalian cells. Radiat. Res. 134:1993;375-382.
19. Held K. D., Biaglow J. E. Mechanisms for the oxygen radical-mediated toxicity of various thiol-containing compounds in cultured mammalian cells. Radiat. Res. 139:1994;15-23.
20. Held K. D., Tuttle S. W., Biaglow J. E. Role of the pentose cycle in oxygen radical-mediated toxicity of the thiol-containing radioprotector dithiothreitol in mammalian cells. Radiat. Res. 134:1993;383-389.
21. Held K. D., Biaglow J. E. Role of Fenton chemistry in thiol-induced toxicity and apoptosis. Radiat. Res. 145:1996;542-553.
22. Biaglow J. E., Jacobson B., Varnes M. E., Held K. D. Cellular oxygen utilization and hypoxia: Interaction of dithiols with cellular electron transfer systems. Longmuir S. Oxygen transport to tissue. VIII:1986;591-601 Plenum, New York.
23. Biaglow J. E., Issels R. W., Gerweck L. E., Varnes M. E., Mitchell J. B., Russo A. Factors influencing the oxidation of cysteamine and other thiols: Implications for hyperthermic sensitization and radiation protection. Radiat. Res. 100:1984;298-308.
24. Kochetkov N. K., Kudrjashov L. I., Chlenov M. A. Radiation chemistry of carbohydrates. 1979;Pergamon, Oxford.
25. Gutteridge J., Toeg D. Iron-dependent free radical damage to DNA and deoxyribose. Separation of TBA-reactive intermediates. Int. J. Biochem. 14:1982;891-893.
26. Kachur, A.; Manevich, Y.; Biaglow, J. E. Role of oxygen in Fenton catalyzed reactions.Free Radic. Biol. Med. (submitted).
27. Winterbourn C. C. Factors that influence the deoxyribose oxidation assay for Fenton reaction products. Free Radic. Biol. Med. 11:1991;353-360.
28. Schulte-Frohlinde D., von Sonntag C. Radiation chemistry of ethylene glycol, meso-erythritol, 2-deoxy-ribose and alkyl phosphates as DNA model compounds. Israel J. Chem. 10:1972;1139-1150.
29. Bucknall T., Edwards H. E., Kemsley K. G., Moore J. S., Phillips G. O. The formation of malonaldehyde in irradiated carbohydrates. Carbohydr. Res. 62:1978;49-59.
30. Cheesman K. H., Beavis A., Esterbauer H. Hydroxyl-radical-induced iron-catalyzed degradation of 2-deoxyribose. Quantitive determination of malondialdehyde. Biochem. J. 252:1988;649-653.
31. Von Sonntag C. The chemical basis of radiation biology. 1987;Taylor & Francis, London.
32. Waravdecar V. S., Saslaw L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of malonaldehyde-thiobarbituric acid reaction. J. Biol. Chem. 234:1959;1945-1950.
33. Wayner D. D. M., Burton G. W., Ingold K. U. The antioxidant efficiency of vitamin C is concentration dependent. Biochim. Biophys. Acta. 884:1986;119-123.
34. Buettner G. R. In the absence of catalytic metals ascorbate does not autooxidize at pH 7: Ascorbate as a test for catalytic metals. J. Biochem. Biophys. Methods. 16:1988;27-40.
35. Maskos Z., Rush J. D., Koppenol W. H. The hydroxylation of the salicylate anion by a Fenton reaction and γ -radiolysis: A consideration of the respective mechanisms. Free Radic. Biol. Med. 8:1990;153-162.
36. Halliwell B., Gutteridge J. M. C. The antioxidants of human extracellular fluids. Arch. Biochem. Biophys. 280:1990;1-8.
37. Sutton H. C., Winterbourn C. C. On the participation of higher oxidation states of iron and copper in Fenton reactions. Free Radic. Biol. Med. 6:1989;53-60.
38. Aruoma O. I., Grootveld M., Halliwell B. The role of iron in ascorbate-dependent deoxyribose degradation. Evidence consistant with site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule. J. Inorg. Biochem. 29:1987;289-299.
39. Nanni E. J., Stallings M. D., Sawyer D. T. Does superoxide ion oxidize catechol, α -tocopherol, and ascorbic acid by direct electron transfer? J. Am. Chem. Soc. 102:1980;4481-4485.