Studies on the site of phosphorylation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase

Journal of Biochemistry

Volumn 121, Issue 4, 1997, Pages 804-810

  Kitani, Takako ^a   Okuno, Saehiko ^a   Fujisawa, Hitoshi ^a  

^a ASAHIKAWA MEDICAL COLLEGE   (Japan)

Author keywords

Calmodulin dependent protein kinase; Cam kinase iv; Cam kinase kinase; Phosphorylation site; Protein phosphorylation

Indexed keywords

ALANINE; ARGININE; LYSINE; PROTEIN KINASE (CALCIUM,CALMODULIN); THREONINE; TRYPSIN;

AMINO ACID SUBSTITUTION; ARTICLE; AUTOPHOSPHORYLATION; ENZYME PHOSPHORYLATION; NONHUMAN; RAT;

EID: 0030893046     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021656     Document Type: Article

References (39)

1. Fujisawa, H. (1990) Calmodulin-dependent protein kinase II. BioEssays 12, 27-29
2. Colbran, R.J. and Suderling, T.R. (1900) Calcium calmodulindependent protein kinase II. Curr. Top. Cell. Regul. 31, 181-221
3. Hanson, P.I. and Schulman, H. (1992) Neuronal Ca calmodulin-dependent protein kinases. Annu. Rev. Biochem. 61, 559-601
4. Nairn, A.C. and Picciotto, M.H. (1994) Calcium calmodulin-dependent protein kinases. Sem. Cancer Biol. 5, 295-303
5. Okuno, S. and Fujisawa, H. (1993) Requiremunt of brain extract for the activity of brain calmodulin-dependent protein kinase IV expressed in Escherichia coli. J. Biochem. 114, 167-170
6. Okuno, S., Kitani, T., and Fujisawa, H. (1994) Purification and characterisation of Ca calmodulin-dependent protein kinase IV kinase from rat brain. J. Biochem. 116, 923-930
7. Tokumitsu, H., Brickey, D.A., Glod, J., Hidaka, H., Sikela, J., and Soderling, T.R. (1994) Activation mechanism for Ca calmodulin-dependent protein kinase IV. Identification of a brain CaM-kinase IV kinase. J. Biol. Chem. 269, 28640-28647
8. Mochizuki, H., Sugita, R., Ito, T., and Hidaka, H. (1993) Phosphorylation of Ca calmodulin-dependent protein kinase V and regulation of its activity. Biochem. Biophys. Res. Commun. 197, 1590-1600
9. Lee, J.C. and Edelman, A.M. (1994) A protein activator of Ca-calmodulin-dependent protein kinase Ia. J. Biol. Chem. 269, 2158-2164
10. Tokumitsu, H., Enslen, H., and Soderling, T.R. (1995) Characterization of a Ca calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium calmodulindependent protein kinase kinase. J. Biol. Chem. 270, 19320-19324
11. Okuno, S., Kitani, T., and Fujisawa, H. (1996) Evidence for the existence of Ca calmodulin-dependent protein kinase IV kinase isoforms in rat brain. J. Biochem. 119, 1176-1181
12. Edelman, A.M., Mitchelhill, K.I., Selbert, M.A., Anderson, K.A., Hook, S.S., Stapleton, D., Goldstein, E.G., Moans, A.R., and Kemp, R.E. (1996) Multiple Ca-calmodulin-dependent protein kinase kinases from rat brain. Purification, regulation by Ca -calmodulin, and partial amino acid sequence. J. Biol. Chem. 271, 10806-10810
13. Okuno, S., Kitani, T., and Fujisawa, H. (1997) Purification and characterization of Ca /calmodulin-dependent protein kinase kinase β from rat cerebellum. J. Biochem. 121, 155-160
14. Sugita, R., Mochizuki, H., Ito, T., Yokokura, H., Kobayashi, R., and Hidaka, H. (1994) Ca /calmodulin-dependent protein kinase kinase cascade. Biochem. Biophys. Res. Commun. 203, 694-701
15. Haribabu, B., Hook, S.S., Selbert, M.A., Goldstein, E.G., Tomhave, E.D., Edelman, A.M., Snyderman, R., and Means, A.R. (1995) Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase. EMBO J. 14, 3679-3686
16. Selbert, M.A., Anderson, K.A., Huang, Q., Goldstein, E.G., Means, A.R., and Edelman, A.M. (1995) Phosphorylation and activation of Ca-calmodulin-dependent protein kinase IV by Ca-calmodulin-dependent protein kinase la kinase. Phosphorylation of threonine 196 is essential for activation. J. Biol. Chem. 270, 17616-17621
17. McDonald, O.B., Merrill, B.M., Bland, M.M., Taylor, L.C.E., and Sahyoun, N. (1993) Site and consequences of the autophosphorylation of Ca calmodulin-dependent protein kinase type "Gr." J. Biol. Chem. 268, 10054-10059
18. Okuno, S., Kitani, T., and Fujisawa, H. (1995) Full activation of brain calmodulin-dependent protein kinase IV requires phosphorylation of the ammo-terminal serine-rich region by calmodulin-dependent protein kinase IV kinase. J. Biochem. 117, 686-690
19. Miyano, O., Kameshita, I., and Fujisawa, H. (1992) Purification and characterization of a brain-specific multifunctional calmodulin-dependent protein kinase from rat cerebellum. J. Biol. Chem. 267, 1198-1203
20. Kitani, T., Okuno, S., and Fujisawa, H. (1995) Inactivation of Ca calmodulin-dependent protein kinase IV by Ca calmodulin and restoration of the activity by Mg EGTA. J. Biochem. 117, 1070-1075
21. Gopalakrishna, R. and Anderson, W.B. (1982) Ca -induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104, 830-836
22. Putkey, J.A., Ts'ui, K.F., Tanaka, T., Lagace', L., Stein, J.P., Lai. E.C., and Means, A.R. (1983) Chicken calmodulin genes. A species comparison of cDNA sequences and isolation of a genomic clone. J. Biol. Chem. 258, 11864-11870
23. Kunkel, T.A., Roberts, J.D., and Zakour, R.A. (1987) Rapid and efficient site-specific mutagenesis without phenotype selection. Methods Enzymol. 154, 367-382
24. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467
25. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff. J.W. (1990) Use of T7 RNA polymerase to direct expra cloned genes. Methods Enzymol. 185, 60-89
26. Corbin, J.D. and Reimann, E.M. (1974) Assay of cyclic dependent protein kinases. Methods Enzymol. 38, 287-29
27. Roskoski, R., Jr. (1983) Assays of protein kinase. Enzymol. 99, 3-6
28. Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227
29. Winston, S.E., Kuller, S.A., and Hurrell, J.G.R. (1987) Current Protocols in Molecular Biology (Ausubel et al., eds.) 2, 10.8.1-10.8.6, Wiley Interscience, Mass
30. Dedman, J.R., Potter, J.D., Jackson, R.L., Johnson, J.D., Means, A.R. (1977) Physicochemical properties of rat testis dependent regulator protein of cyclic nucleotide phosphodie ase. Relationship of Ca -binding, conformational changes, phosphodiesterase activity. J. Biol. Chem. 252, 8415-8422
31. Dedman, J.R., Jackson, R.L., Schreiber, W.E., and Means, (1978) Sequence homology of the Ca -dependent regulator cyclic nucleotide phosphodiesterase from rat testis with Ca -binding proteins. J. Biol. Chem. 253, 343-346
32. Watterson, D.M., Sharief, F., and Vanaman, T.C. (1980) complete amino acid sequence of the Ca -dependent modula protein (calmodulin) of bovine brain. J. Biol. Chem. 255, 9-975
33. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
34. Peterson, G.L. (1977) A simplification of the protein method of Lowry et al. which is more generally applicable. Biochem. 83, 346-356
35. Kameshita, I. and Fujisawa, H. (1995) Preparation and char terization of calmodulin-dependent protein kinase IV (CaM- nase IV) free of CaM-kinase IV kinase from rat cerebral cort J. Biochem. 117, 85-90
36. Means, A.R., Cruzalegui, F., LeMagueresse, H., Needle D.S., Slaughter, G.R., and Ono, T. (1991) A novel Ca modulin-depcndent protein kinase and a male germ cell-speci calmodulin-binding protein are derived from the same gene. Mol. Cell. Biol. 11, 3960-3971
37. Ohmstede, C.-A., Bland, M.M., Merrill, B.M., and Sahyoun, N. (1991) Relationship of genes encoding Ca calmodulin-dependent protein kinase Gr and calspermine: A gene within a g Proc. Natl. Acad. Sci. USA 88, 5784-5788
38. Cooper, J.A., Sefton, B.M., and Hunter, T. (1983) Detection and quantification of phosphotyrosine in proteins. Methods Enzymol. 99, 387-402
39. Chatila, T., Anderson, K.A., Ho, N., and Means, A.R. (1996) unique phosphorylation-dependent mechanism for the activation of Ca calmodulin-dependent protein kinase type IV Gr. J. Biol. Chem. 271, 21542-21548