Purification and characterization of Ca2+/calmodulin-dependent protein kinase kinase β from rat cerebellum

Journal of Biochemistry

Volumn 121, Issue 1, 1997, Pages 155-160

  Okuno, Sachiko ^a   Kitani, Takako ^a   Fujisawa, Hitoshi ^a  

^a ASAHIKAWA MEDICAL COLLEGE   (Japan)

Author keywords

Brain; CaM kinase kinase; Isoform; Protein kinase kinase

Indexed keywords

BRAIN ENZYME; ENZYME ANTIBODY; ISOENZYME; PHOSPHOTRANSFERASE; PROTEIN KINASE (CALCIUM,CALMODULIN); CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE KINASE BETA; CALCIUM CALMODULIN-DEPENDENT PROTEIN KINASE KINASE BETA; PROTEIN SERINE THREONINE KINASE;

ANIMAL TISSUE; ARTICLE; CEREBELLUM; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; ENZYME SPECIFICITY; ENZYMOLOGY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PHOSPHORYLATION;

ANIMALIA;

AMINO ACID SEQUENCE; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CEREBELLUM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; RATS; SUBSTRATE SPECIFICITY;

EID: 0030637371     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021560     Document Type: Article

References (40)

1. Fujisawa, H. (1990) Calmodulin-dependent protein kinase II, BioEssays 12, 27-29
2. Colbran, R.J. and Soderling, T.R. (1990) Calcium/calmodulin-dependent protein kinase II. Curr. Top. Cell. Regul. 31, 181-221
3. 2+/calmodulin-dependent protein kinases. Annu. Rev. Biochem. 61, 559-601
4. Nairn, A.C. and Picciotto, M.R. (1994) Calcium/calmodulin-dependent protein kinases. Semin. Cancer Biol. 5, 295-303
5. Okuno, S. and Fujisawa, H. (1993) Requirement of brain extract for the activity of brain calmodulin-dependent protein kinase IV expressed in Escherichia coli. J. Biochem. 114, 167-170
6. 2+ /calmodulin-dependent protein kinase IV kinase from rat brain. J. Biochem. 116, 923-930
7. 2+/calmodulin-dependent protein kinase V and regulation of its activity. Biochem. Biophys. Res. Commun. 197, 1595-1600
8. 2+ -calmodulin-dependent protein kinase Ia. J. Biol. Chem. 269, 2158-2164
9. 2+/calmodulin-dependent protein kinase cascade. Molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase. J. Biol. Chem. 270, 19320-19324
10. 2+/calmodulin dependent protein kinase IV kinase isoforms in rat brain. J. Biochem. 119, 1176-1181
11. Hashimoto, Y. and Soderling, T.R. (1987) Calcium/calmodulin-dependent protein kinase II and calcium,'phospholipid-dependent protein kinase activities in rat tissues assayed with a synthetic peptide. Arch. Biochem. Biophys. 252, 418-425
12. Miyano, O., Kameshita, I., and Fujisawa, H. (1992) Purification and characterization of a brain-specific multifunctional calmodulin-dependent protein kinase from rat cerebellum. J. Biol. Chem. 267, 1198-1203
13. 2+/EGTA. J. Biochem. 117, 1070-1075
14. 2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104, 830-836
15. Putkey, J.A., Ts'ui, K.F., Tanaka, T., Lagace', L., Stein, J.P., Lai, E.C., and Means, A.R. (1983) Chicken calmodulin genes. A species comparison of cDNA sequences and isolation of a genomic clone. J. Biol. Chem. 258, 11864-11870
16. Picciotto, M.R., Czernik, A.J., and Nairn, A.C. (1993) Calcium/ calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. J. Biol. Chem. 268, 26512-26521
17. Roskoski, R., Jr. (1983) Assays of protein kinase. Methods Enzymol. 99, 3-6
18. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
19. Winston, S.E., Fuller, S.A., and Hurrell, J.G.R. (1987) Current Protocols in Molecular Biology (Ausubel et al., eds.) Vol. 2, 10.8.1-10.8.6, Wiley Interscience, Mass.
20. Kameshita, I. and Fujisawa, H. (1989) A sensitive method for detection of calmodulin-dependent protein kinase II activity in sodium dodecyl sulfate-polyacrylamide gel. Anal. Biochem. 183, 139-143
21. Layne, E. (1957) Spectrophotometric and turbidimetric methods for measuring proteins. Methods Enzymol 3, 447-454
22. 2+-binding, conformational changes, and phosphodiesterase activity. J. Biol. Chem. 252, 8415-8422
23. 2+-binding proteins. J. Biol. Chem. 253, 343-346
24. 2+-dependent modulator protein (calmodulin) of bovine brain, J. Biol. Chem. 255, 962-975
25. Lowry, O.H., Rosebrough, N.J., Fair, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
26. Peterson, G.L. (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346-356
27. 2+/calmodulin-dependent protein kinase IV from rat brain, expressed in insect cells, and expressed in Escherichia coli. J. Biochem. 118, 364-370
28. 2+/calmodulin-dependent protein kinase kinase α in the rat central nervous system: an immunohistochemical study. Neurosci Lett. 204, 61-64
29. Rangel-Aldao, R., Kupiec, J.W., and Rosen, O.M. (1979) Resolution of the phosphorylated and dephosphorylated cAMP-binding proteins of bovine cardiac muscle by affinity labeling and two-dimensional electrophoresis. J. Biol. Chem. 254, 2499-2508
30. Bennett, M.K., Erondu, N.B., and Kennedy, M.B. (1983) Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain. J. Biol. Chem. 258, 12735-12744
31. 2+/calmodulin-dependent protein kinase from rat brain. Biochemistry 23, 5495-5504
32. Yamauchi, T. and Fujisawa, H. (1985) Self-regulation of calmodulin-dependent protein kinase II and glycogen synthase kinase by autophosphorylation. Biochem. Biophys. Res. commun. 129, 213-219
33. Kameshita, I. and Fujikawa, H. (1993) Autophosphorylation of calmodulin-dependent protein kinase IV from rat cerebral cortex. J. Biochem. 113, 583-590
34. 2+-calmodulin, and partial amino acid sequence. J. Biol. Chem. 271, 10806-10810
35. 2+/calmodulin-dependent protein kinase V: Tissue distribution and immunohistochemical localization in rat brain. Arch. Biochem. Biophys. 312, 278-284
36. Picciotto, M.R., Zoli, M., Bertuzzi, G., and Nairn, A.C. (1995) Immunochemical localization of calcium/calmodulin-dependent protein kinase I. Synapse 20, 75-84
37. 2+/calmodulin-dependent protein kinase type Gr in rat cerebellar cortex, Proc. Natl. Acad. Sci. USA 88, 2850-2853
38. 2+/calmodulin-dependent protein kinase type IV in the peripheral ganglia and paraganglia of developing and mature rats. Brain Res. 666, 173-181
39. 2+/calmodulin-dependent protein kinase IV in the rat central nervous system: light and electron microscopic observations. Neuroscience 68, 181-194
40. 2+ calmodulin-dependent protein kinase enriched in cerebellar granule cells. Identification of a novel neuronal calmodulin-dependent protein kinase. J. Biol. Chem. 264, 5866-5875