Flow cytometric measurement of DNA fragmentation in cell death by apoptosis. New techniques to evaluate DNA repair for pathologists;Mise en evidence de la fragmentation d'ADN par cytometrie en flux au cours de la mort cellulaire par apoptose. Nouvelles techniques d'evaluation de l'etat de l'ADN pour le pathologiste

Annales de Pathologie

Volumn 17, Issue 1, 1997, Pages 61-66

  Lizard, G ^a   Miguet, C ^a   Gueldry, S ^a   Monier, S ^a   Gambert, P ^a  

^a HÔPITAL DU BOCAGE   (France)

Author keywords

apoptosis; DNA fragmentation; flow cytometry

Indexed keywords

APOPTOSIS; ARTICLE; DNA REPAIR; FLOW CYTOMETRY; HUMAN; TECHNIQUE; GENETICS; IN SITU HYBRIDIZATION; REVIEW;

DNA FRAGMENT;

APOPTOSIS; DNA FRAGMENTATION; DNA REPAIR; FLOW CYTOMETRY; HUMANS; IN SITU HYBRIDIZATION;

EID: 0030892946     PISSN: 02426498     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. BOWEN ID. Apoptosis or programmed cell death ? Cell Biol Int 1993 ; 17 : 365-80.
2. LIZARD G, FOURNEL S, GENESTIER L et al. Kinetics of plasma membrane and mitochondrial alterations in cells undergoing apoptosis. Cytometry 1995 ; 21 : 275-83.
3. VERMES I, HAANEN C, STEFFENS-NAKKEN H, REUTELINGSPERGER C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 1995; 184 : 39-51.
4. ARENDS MJ, MORRIS RG, WYLLIE AH. Apoptosis. The role of endonuclease. Am J Pathol 1990 ; 136 : 593-608.
5. DIVE C, WATSON JV, WORKMAN P. Multiparametric analysis of cell membrane permeability by two colours flow cytometry with complementary fluorescent probes. Cytometry 1990 ; 11 : 244-52.
6. ORMEROD MG, SUN XM, BROWN D, SNOWDEN RT, COHEN GM. Quantification of apoptosis and necrosis by flow cytometry. Acta Oncol 1993 ; 32 : 417-24.
7. PETIT PX, LECOEUR H, ZORN E, DAUGET C, MIGNOTTE B, GOUGEON ML. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J Cell Biol 1995 ; 130 : 157-67.
8. ESCARGUEIL-BLANC I, SALVAYRE R. NEGRE-SALVAYRE A. Necrosis and apoptosis induced by oxidized low density lipoproteins occur through two calcium-dependent pathways in Iymphoblastoid cells. FASEB J 1994 ; 8 : 1075-80.
9. LEDDA-COLUMBANO GM, CONI P, CURTO M et al. Induction of two different modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am J Pathol 1991 ; 139 : 1099-1109.
10. GERSCHENSON LE, ROTELLO RJ. Apoptosis: a different type of cell death. FASEB J 1992 ; 6 : 2450-5.
11. SALOMON RN, DIAZ-CANO S. Introduction to apoptosis. Diag Mol Pathol 1995 ; 4 : 235-8.
12. SCHWARTZ SM, BENNETT MR. Death by any other name. Am J Pathol 1995 ; 147 : 229-34.
13. BRUNO S, LASSOTA P, GIARETTI W, DARZYNKIEWICZ Z. Apoptosis of rat thymocytes by prednisolone, camptothecin or teniposide is selective to GO cells and is prevented by inhibitors of proteases. Oncology Res 1992 ; 4 : 29-35.
14. GORCZYCA W, BRUNO S, DARZYNKIEWICZ RJ, GONG J, DARZYNKIEWICZ Z. DNA strand breaks occuring during apoptosis: their early in situ detection by the terminal deoxynucleotidyl transferase and nick translation assays and prevention by serine protease inhibitors, Int J Oncol 1992 ; 1 : 639-48.
15. COHEN GM, SUN XM, SNOWDEN RT, DINSDALE D, SKILLETER DN. Key morphological features of apoptosis may occur in the absence of internucleosomal DNA fragmentation. Biochem J 1992; 286 : 331-4.
16. OBERHAMMER F, WILSON JW, DIVE C et al. Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J 1993 ; 12 : 3679-84.
17. ELLIS HM, HORVITZ HR. Genetic control of programm cell death in the nematode C elegans. Cell 1986 ; 44 : 817-29.
18. HINCHLIFFE JR. Cell death in embryogenesis. In : Bowen ID, Lockshin RA. eds. Cell death in biology and pathology. New York, Chapmann and Hall, 1981 : 35-78.
19. HICKMAN JA. Apoptosis induced by anticancer drugs. Cancer Metast Rev 1992 ; 11 : 121-39.
20. BARRETT JC, PRESTON G. Apoptosis and cellular senescence: forms of irreversible growth arrest. In : Tomei LD, Cope FO, eds. Apoptosis II: The molecular basis of apoptosis in disease. New York: Cold Spring Harbor Laboratory Press, 1994 : 253-81.
21. WILLIAMS GT, SMITH CA, MC CARTHY NJ, GRIMES EA. Apoptosis: final control point in cell biology. Trends Cell Biol 1992 ; 2 : 263-7.
22. WYLLIE AH, KERR JFR, CURRIE AR. Cell death: the significance of apoptosis. Int Rev Cytol 1980 ; 68 : 251-306.
23. COLUMBANO A. Cell death: current difficulties in discriminating apoptosis from necrosis in the context of pathological process in vivo. J Cell Biochem 1995 ; 58 : 181-90.
24. CARBONARI M, CIBATI M, FIORILLI M. Measurement of apoptotic cells in peripheral blood. Cytometry (Communications in Clinical Cytometry) 1995 ; 22 : 161-7.
25. DARZYNKIEWICZ Z, BRUNO S, DEL BINO G et al. Features of apoptotic cells measured by flow cytometry. Cytometry 1992 ; 13 : 795-808.
26. ORMEROD MG, COLLINS MKL, RODRIGUEZ-TARDUCHY G. ROBERTSON D. Apoptosis in interleukin-3 dependent haemopoietic cells. Quantification by flow cytometric methods. J Immunol Methods 1992 ; 153 : 57-65.
27. AFANASYEV VN, KOROL BA, MATYLEVICH NP, PECHATNIKOV VA, UMANSKY SR. The use of flow cytometry for the investigation of cell death. Cytometry 1993 ; 14 : 603-9.
28. NICOLETTI I, MIGLIORATI G, PAGLIACCI MC, GRIGNANI F, RICCARDI C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol. Methods 1991, 139 : 271-9.
29. ZAMAI I, FALCIERI G, ZAULI G, CATALDI A, VITALE M. Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 1993 ; 14 : 891-7.
30. TELFORD WG, KING LE, FRAKER PJ. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 1992 ; 13 : 137-43.
31. ELSTEIN KH, THOMAS DJ, ZUCKER RM. Factors affecting flow cytometric detection of apoptotic nuclei by DNA analysis. Cytometry 1995 ; 21 : 170-6.
32. FOURNEL S, GENESTIER L, ROUAULT JP et al. Apoptosis without decrease of cell DNA content. FEBS Letters 1995 ; 367 : 188-92.
33. ZAMAI L, FALCIERI E, MARHEFKA G, VITALE M. Supravitale exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentation. Cytometry 1996 ; 23 : 303-11.
34. LYONS AB, SAMUEL K, SANDERSON A, MADDY AH. Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry Cytometry 1992 ; 13 : 809-21.
35. VITALE M, ZAMAI L, MAZZOTI G, CATALDI A, FALCIERI E. Differential kinetic of propidium iodide uptake in apoptotic and necrotic thymocytes. Histochemistry 1993 ; 100 : 223-9.
36. COHEN JJ, DUKE RC. Glucocorticoid activation of a calcium-dependent endonuclease in thymocytes nuclei leads to cell death. J Immunol 1984 ; 132 : 38-42.
37. GAVRIELI Y, SHERMAN Y, BEN SASSON SA. Identification of programmed cell death in situ via specific labelling of nuclear DNA fragmentation. J Cell Biol 1992 ; 119 : 493-501.
38. GOLD R, SCHMIED M, GIEGRICH G et al. Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques. Lab Invest 1994 ; 71 : 219-25.
39. LI X, JAMES WM, TRAGANOS F, DARZYNKIEWICZ Z. Application of biotin, digoxigenin or fluorescein conjugated deoxynucleotides to label DNA strand breaks for analysis of cell proliferation and apoptosis using flow cytometry. Biotech Histochem 1995 ; 70 : 234-42.
40. PIQUERAS B, AUTRAN B, DEBRE P, GOROCHOV G. Detection of apoptosis at the single-level by direct incorporation of fluorescein-dUTP in DNA strand breaks. Biotechniques 1996 ; 17 : 32-8.
41. BROMIDGE TJ, HOWE DJ, JOHNSON SA, PHILLIPS MJ. Adaptation of the TdT assay for semi-quantitative flow cytometric detection of DNA strand breaks. Cytometry 1995 ; 20 : 257-60.
42. DOUGLAS RS, TARSHIS AD, PLETCHER CH, NOWELL PC, MOORE JS. A simplified method for the coordinate examination of apoptosis and surface phenotype of murine lymphocytes. J Immunol Methods 1995; 188 : 219-28.
43. MUNDLE SD, RAZA A. The two in situ techniques do not differentiate between apoptosis and necrosis but rather reveal distinct patterns of DNA fragmentation in apoptosis. Lab Invest 1995 ; 72 : 611-2.
44. FELDMANN G. L'apoptose ou la mort cellulaire programmée. Ann. Pathol 1995 ; 15 : 92-109.
45. LIZARD G, DECKERT V, DUBREZ L, MOISANT M. GAMBERT P, LAGROST L. Induction of apoptosis in endothelial cells treated with cholesterol oxides. Am J Pathol 1996 ; 148 : 1625-38.