Isolation of a bovine full length cytochrome P450 (CYP3A) cDNA sequence and its functional expression in V79 cells

Environmental Toxicology and Pharmacology

Volumn 3, Issue 1, 1997, Pages 17-24

  Natsuhori, Masahiro ^a   Van Raak, Marcel ^a   Ligtenberg, Margot ^a   Kleij, Livio ^a   Ten Berge, Derk ^a   Zweers Zeilmaker, Wilma M ^a   De Groene, Els M ^a   Van Miert, Adelbert S J P A M ^a   Witkamp, Renger F ^a   Horbach, G Jean ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Bovine; cDNA sequence; CYP3A; Cytochrome P450; Expression; V79 cells

Indexed keywords

CYTOCHROME P450; OXYGENASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATTLE; CELL STRAIN V 79; DNA SEQUENCE; GOAT; LIVER; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0030891803     PISSN: 13826689     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1382-6689(96)00133-0     Document Type: Article

References (38)

1. Aoyama, T., S. Yamano, D.J. Waxman, D.P. Lapenson, U.A. Meyer, V. Fischer, R. Tyndale, T. Inaba, W. Kalow, H.V. Gelboin and F.J. Gonzalez, 1989, Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver: cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine, J. Biol. Chem. 264, 10 388.
2. Beaune, P.M., D.R. Umbenhauer, R.W. Bork, R.S. Lloyd and F.P. Guengerich, 1986, Isolation and sequence determination of acDNA clone related to human cytochrome P-450 nifedipine oxidase. Proc. Natl. Acad. Sci. USA 83, 8064.
3. Back, D.J., P. Stevenson and J.F. Tjia, 1989, Comparative effects of two antimycotic agents, ketoconazole and terbinafine, on the metabolism of tolbutamide, ethinyloestradiol, cyclosporin and ethoxycoumarin by human liver microsomes in vitro, Br. J. Clin. Pharmacol. 28, 166.
4. Ciaccio, P.J., P.E. Graves, D.P. Bourque, B. Glinsmann-Gibson and J.R. Halpert, 1991, cDNA and deduced amino acid sequences of a dog liver cytochrome P-450 of the IIIA gene subfamily, Biochim. Biophys. Acta. 1088, 319.
5. Cullin, C. and D. Pompon, 1988, Synthesis of functional mouse cytochromes P-450 P1 and chimeric P-450 P3-1 in the yeast Saccharomyces cerevisiae, Gene 65, 203.
6. Dalet, C., P. Clair, M. Daujat, P. Fort, J.-M. Blanchard and P. Maurel, 1988, Complete sequence of P450 3c cDNA and presence of two mRNA species with 3′ untranslated regions of different lengths, DNA 7, 39.
7. De Groene, E.M., S.M. Nijmeijer, G.J.M.J. Horbach and R.F. Witkamp, 1995, Tiamulin selectively inhibits human CYP3A4 activity in a NIH/3T3 cell line, stably expressing CYP3A4 cDNA, Biochem. Pharmacol. 50, 771.
8. Fink-Gremmels, J. and A.S.J.P.A.M. van Miert, 1994, Veterinary drugs: disposition, biotransformation and risk evaluation, Analyst 119, 2521.
9. Gonzalez, F.J., 1989, The molecular biology of cytochrome P450s. Pharmacol., Rev. 40, 243.
10. Gonzalez, F.J., D.W. Nebert, J.P. Hardwick and C.B. Kasper, 1985, Complete cDNA and protein sequence of a pregnenolone 16a-carbonitrile-inducible cytochrome P-450. a representative of a new gene family. J. Biol. Chem. 260, 7435.
11. Gonzalez, F.J., B.J. Song and J.P. Hardwick, 1986, Pregnenolone 16a-carbonitrile-inducible P-450 gene family: gene conversion and different regulation, Mol. Cell Biol. 6, 2969.
12. Graham, F.L. and A.J. Van der Eb, 1973, A new technique for the assay of infectivity of human adenovirus 5 DNA, Virology 52, 456.
13. Guengerich, F.P., 1989, Characterization of human microsomal cytochrome P-450 enzymes, Ann. Rev. Pharmacol. Toxicol. 29, 241.
14. Itoh, K., M. Satoh, Y. Abe, H. Hashimoto, T. Yanagimoto and T. Kamataki, 1994, A novel form of mouse cytochrome P450 3A (Cyp3a16): its cDNA cloning and expression in fetal liver, Eur. J. Biochem. 226, 877.
15. Kaddouri, M., N. Brasset, M. Alvinerie, C. Eeckhoutte, C. Bonfils, J. Derancourt and P. Galtier, 1992, Ontogenetic development of liver progesterone metabolism in female sheep: contribution of cytochrome P450 2B and 3A subfamilies, J. Steroid Biochem. Mol. Biol. 42, 499.
16. Komori, M., O. Kikuchi, T. Sakuma, J. Funaki, M. Kitada and T. Kamataki, 1992, Molecular cloning of monkey liver cytochrome P450 cDNAs: similarity of the primary sequences to human cytochromes P450, Biochim. Biophys. Acta 1171, 141.
17. Komori, M., K. Nishio, H. Ohi, M. Kitada and T. Kamataki, 1989, Molecular cloning and sequence analysis of cDNA containing the entire coding region for human fetal liver cytochrome P-450, J. Biochem. (Tokyo) 105, 161.
18. Lazard, D., N. Tal, M. Rubinstein, M. Khen, D. Lancet and K. Zupko, 1990, Identification and biochemical analysis of novel olfactory-specific cytochrome P450IIA and UDP-glucuronosyl transferase, Biochemistry 29, 7433.
19. Miller, A.D. and G.J. Rosman, 1989, Improved retroviral vectors for gene transfer and expression, Biotechniques 7, 980.
20. Molowa, D.T., E.G. Schuetz, S.A. Wrighton, P.B. Watkins, P. Kremers, G. Mendez-Picon, G.A. Parker and P.S. Guzelian, 1986, Complete cDNA sequence of a cytochrome P-450 inducible by glucocorticoids in human liver, Proc. Natl. Acad. Sci. USA 83, 5311.
21. Nelson, D.R., L. Koymans, T. Kamataki, J.J. Stegeman, R. Feyereisen, D.J. Waxman et al., 1996, P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature, Pharmacogenetics 6, 1.
22. Pineau, T., P. Galtier, C. Bonfils, J. Derancourt and P. Maurel, 1990, Purification of a sheep liver cytochrome P-450 from the P450IIIA subfamily: its contribution to the N-dealkylation of veterinary drugs, Biochem. Pharmacol. 39, 901.
23. Sanger, F., S. Nicklen and A.R. Coulson, 1977, DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci. USA 74, 5463.
24. Schneider, A., W.A. Schmalix, V. Siruguri, E.M. de Groene, G.J. Horbach, B. Kleingeist, D. Lang, R. Böcker, C. Belloc, P. Beaune, H. Greim and J. Doehmer, 1996, Stable expression of human cytochrome P450 3A4 in conjunction with human NADPH-cytochrome P450 oxidoreductase in V79 chinese hamster cells, Arch. Biochem. Biophys. 332, 295.
25. Strotkamp, D., P.H. Roos and W.G. Hanstein, 1994, A novel CYP3 from female rats, Biochem. Biophys. Acta 1260, 341.
26. Teixeira, J. and G. Gil., 1991, Cloning, expression, and regulation of lithocholic acid 6β-hydroxylase, J. Biol. Chem. 266, 21 030.
27. Tsuneoka, Y., Y. Matsuo, R. Higuchi and Y. Ichikawa, 1992, Characterization of the cytochrome P-450IID (CYP2D) subfamily in bovine liver: nucleotide sequences and microheterogeneity, Eur. J. Biochem. 208, 739.
28. Vanden Bossche, H., 1992, Inhibitors of P-450 dependent steroid biosynthesis: from research to medical treatment, J. Steroid Biochem. Mol. Biol. 43, 1003.
29. Van 't Klooster, G.A.E., 1992, Drug metabolism in ruminants: an in vitro approach. Thesis, Utrecht University, The Netherlands.
30. Van 't Klooster, G.A.E., B.J. Blaauboer, J. Noordhoek and A.S.J.P.A.M. Van Miert, 1993a, Sulfadimidine metabolism in vitro: II. comparative studies in cultured rat, goat, sheep and cattle hepatocytes, J. Vet. Pharmacol. Ther. 16, 454.
31. Van 't Klooster, G.A.E., B.J. Blaauboer, J. Noordhoek and A.S.J.P.A.M. Van Miert, 1993b, Cytochrome P450 induction and metabolism of alkoxyresorufins, ethylmorphine and testosterone in cultured hepatocytes from goats, sheep and cattle, Biochem. Pharmacol. 46, 1781.
32. Van 't Klooster, G.A.E., G.J.M.J. Horbach, M. Natsuhori, B.J. Blaauboer, J. Noordhoek and A.S.J.P.A.M. van Miert, 1993c, Hepatic cytochrome P450 induction in dwarf goats. Effects of model inducers on the metabolism of alkoxyresorufins, testosterone and ethylmorphine, and on apoprotein and mRNA levels, Biochem. Pharmacol. 45, 113.
33. Waxman, D.J., A. Ko and C. Walsh, 1983, Regioselectivity and stereoselectivity of androgen hydroxylations catalyzed by cytochrome P-450 isozymes purified from phenobarbital-induced rat liver, J. Biol. Chem. 258, 11 937.
34. Witkamp, R.F., H.I. Yun, G.A.E. Van 't Klooster, J.F. Van Mosel, M. Van Mosel, J.M. Ensink, J. Noordhoek and A.S.J.P.A.M. Van Miert, 1992, Comparative aspects and sex differentiation of plasma sulfamethazine elimination and metabolite formation in rats, rabbits, dwarf gloats, and cattle, Am. J. Vet. Res. 53, 1830.
35. Witkamp, R.F., S.M. Nijmeijer, M. Monshouwer and A.S.J.P.A.M. van Miert, 1995, The antibiotic tiamulin is a potent inducer and inhibitor of cytochrome P4503A via the formation of a stable metabolic intermediate complex. Studies in primary hepatocyte cultures and liver microsomes of the pig, Drug Metab. Dispos. 23, 542.
36. Yanagimoto, T., S. Itoh, D. Muller-Enoch and T. Kamataki, 1992, Mouse liver cytochrome P-450 (P-450IIIAM1): its cDNA cloning and inducibility by dexamethasone, Biochim. Biophys. Acta. 1130, 329.
37. Zaphiropoulos, P.G., A. Skantz, M. Eliasson and M.B. Ahlberg, 1995, Cytochrome P450 genes expressed in porcine ovaries: identification of novel forms, evidence for gene conversion, and evolutionary relationships, Biochem. Biophys. Res. Commun. 212, 433.
38. Zeilmaker, W.M., G.A.E. Van 't Klooster, J. Fink Gremmels-Gehrmann, A.S.J.P.A.M. van Miert and G.J.M.J. Horbach, 1994, cDNA and deduced amino acid sequences of a dwarf goat liver cytochrome P450-fragment belonging to the CYP2C gene subfamily, Biochem. Biophys. Res. Commun. 200, 120.