Mössbauer and EPR evidence for an all-ferrous Fe4S4 cluster with S = 4 in the Fe protein of nitrogenase

Journal of the American Chemical Society

Volumn 119, Issue 37, 1997, Pages 8730-8731

  Angove, Hayley C ^a   Yoo, Sun Jae ^b   Burgess, Barbara K ^a   Münck, Eckard ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FERROUS ION; FERROUS SULFATE; IRON DERIVATIVE; NITROGENASE;

ARTICLE; DEGRADATION; ELECTRON SPIN RESONANCE; MOSSBAUER SPECTROSCOPY; NITROGEN FIXATION; REACTION ANALYSIS;

EID: 0030883525     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9712837     Document Type: Article

References (18)

1. Burgess, B. K.; Lowe, D. J. Chem. Rev. 1996, 96, 2983-3012.
2. Howard, J. B.; Rees, D. C. Chem. Rev. 1996, 96, 2965-2982.
3. Watt, G. D.; Reddy, K. R. N. J. Inorg. Biochem. 1994, 53, 281-294.
4. Thiriot, D. M.S. Thesis, Brigham Young University, Provo, UT, 1995.
5. 3 suggested that the electron might be localized on sulfur atoms.
6. 4 cluster with phosphine ligands has been reported.
7. Goh, C.; Segal, B. M.; Huang, J.; Lang, S. R.; Holm, R. H. J. Am. Chem. Soc. 1996, 118, 11844-11853.
8. 9 and that the fully reduced protein is stable for days at room temperature.
9. Burgess, B. K.; Jacobs, D. B.; Stiefel, E. I. Biochim. Biophys. Acta. 1980, 614, 196-209.
10. Seefeldt, L. C; Ensign, S. A. Anal. Biochim. 1994,227, 379-386.
11. Lanzilotta, W. N.; Holz, R. C.; Seefeldt, L. C. Biochemistry 1995, 34, 15646-15653.
12. Lindahl, P. A.; Day, E. P.; Kent, T. A.; Orme-Johnson, W. H.; Münck, E. J. Biol. Chem. 1985, 260, 11160-11173.
13. Münck, E.; Surerus, K. K.; Hendrich, M. P. Methods Enqmol. 1993, 227, 463-479.
14. -1, the Earth's magnetic field induces for the ground doublet hyperfine fields of 0.3-0.5 T, leading to broadening of the lines. This broadening is essentially absent at 40 K (Figure 1A).
15. 2.5+ pair in the 2+ state (0.45 mm/s).
16. Surerus, K. K.; Hendrich, M. P.; Christie, P. D.; Rottgardt, D.; OrmeJohnson, W. H.; Münck, E. J. Am. Chem. Soc. 1992, 114, 8579-8590.
17. Eaton, W. A.; Lovenberg, W. In Iron Sulfur Proteins; Lovenberg, W., Ed.; Academic Press: New York, 1973; Vol. II, Chapter 3.
18. Im, S-C.; Kohzuma, T.; McFarlane, W.; Gaillard, J.; Sykes, A. G. Inorg. Chem. 1997, 36, 1388-1396.