Absence of androgen-mediated transcriptional effects in osteoblastic cells despite presence of androgen receptors

Bone

Volumn 21, Issue 1, 1997, Pages 49-56

  Czerwiec, F S ^a,b   Liaw, J J ^a   Liu, S B ^a   Perez Stable, C ^a   Grumbles, R ^a   Howard, G A ^a   Roos, B A ^a   Burnstein, Kerry L ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

Androgen receptor; Bone; Dihydrotestosterone; Gene expression; Osteoblast; Osteosarcoma

Indexed keywords

ANDROGEN; ANDROGEN RECEPTOR; ANDROSTANOLONE; COMPLEMENTARY DNA; ESTROGEN; MESSENGER RNA; PARATHYROID HORMONE;

ARTICLE; BONE CELL; BONE MATURATION; CELL DIFFERENTIATION; CELL FUNCTION; CELL LINE; CELL STIMULATION; CONTROLLED STUDY; EXPRESSION VECTOR; GENE ACTIVATION; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; LIGAND BINDING; OSTEOBLAST; OSTEOPOROSIS; PROTEIN DNA INTERACTION; RECEPTOR BINDING; REPORTER GENE; RIBONUCLEASE PROTECTION ASSAY; SHORT STATURE;

ANDROGENS; ANIMALS; BINDING, COMPETITIVE; BLOTTING, NORTHERN; BLOTTING, WESTERN; BONE NEOPLASMS; CELL DIFFERENTIATION; CELL LINE; CELLS, CULTURED; DNA; FEMALE; GENE EXPRESSION REGULATION; GENES, REPORTER; HUMANS; MALE; OSTEOBLASTS; OSTEOSARCOMA; PARATHYROID HORMONE; RADIOLIGAND ASSAY; RECEPTORS, ANDROGEN; RIBONUCLEASES; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 0030878043     PISSN: 87563282     EISSN: None     Source Type: Journal    
DOI: 10.1016/S8756-3282(97)00079-3     Document Type: Article

References (72)

1. Adler, A. J., Scheller, A. Y. H., and Robins, D. M. Multiple components of a complex androgen-dependent enhancer. Mol Endocrinol 5:1587-1596; 1991.
2. Albright, F. and Reifenstein, E. C. Metabolic Bone Disease: Osteoporosis. The Parathyroid Glands and Metabolic Bone Disease. Baltimore: Williams and Wilkins; 1948; 145.
3. Baran, D. T., Sorensen, A. M., Shalhoub, V., Owen, T., Stein, G., and Lian, J. The rapid nongenomic actions of 1α,25-dihydroxy vitamin D3 modulate the hormone induced increments in osteocalcin gene transcription in osteoblast-like cells. J Cell Biochem 50:124-129; 1992.
4. Bellido, T., Jilka, R. L., Boyce, B. F., Girasole, G., Broxmeyer, H., Dalrymple, S. A., Murray, R., and Manolagas, S. C. Regulation of interleukin-6, osteoclastogenesis, and bone mass by androgens: The role of the androgen receptor. J Clin Invest 95:2886-2895; 1995.
5. 1(1)-procollagen and transforming growth factor-B steady state messenger ribonucleic acid levels in human osteoblast-like osteosarcoma cells. Endocrinology 128:2723-2730; 1991.
6. Bodine, P. V. N., Riggs, B. L., and Spelsberg, T. C. Regulation of c-fos expression and TGF-production by gonadal and adrenal androgens in normal human osteoblastic cells. J Ster Biochem Mol Biol 52:149-158; 1995.
7. Bruch, H., Wolf, L., Budde, R., Romalo, G., and Schweikert, H. Androstenedione metabolism in cultured human ostoblast-like cells. J Clin Endocrinol Metab 75:101-105; 1992.
8. Choi, S. K., Han, S. W., Kim, D. H., and de Lignieres, B. Transdermal dihydrotestosterone therapy and its effects on patients with microphallus. J Urol 150:657-660; 1993.
9. Colvard, D. S., Eriksen, E. F., Keeting, P. E., Wilson, E. M., Lubahn, D. B., French, F. S., Riggs, B. L., and Spelsberg, T. C. Identification of androgen receptors in normal human osteoblast-like cells. Proc Natl Acad Sci USA 86:854-857; 1989.
10. Corvol, M. Evidence for a direct in vitro action of sex steroids on rabbit cartilage cells during skeletal growth: Influence of age and sex. Endocrinology 120:1422-1429; 1987.
11. Ernst, M., Parker, M. G., and Rodan, G. A. Functional estrogen receptors in osteoblastic cells demonstrated by transfection with a reporter gene containing an estrogen response element. Mol Endocrinol 5:1597-1606; 1991.
12. Finkelstein, J. S., Neer, R. M., Biller, B. M. K., Crawford, J. D., and Klibanski, A. Osteopenia in men with a history of delayed puberty. New Engl J Med 326:600-604; 1992.
13. Fukayama, S. and Tashjian A. H. Direct Modulation by androgens of the response of human bone cells (SaOS-2) to human parathyroid hormone (PTH) and PTH-related protein. Endocrinology 125:1789-1794; 1989.
14. Gorman, C. M., Moffat, L. F., and Howard, B. H. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol 2:1044-1051; 1982.
15. Grino, P. B., Isidro-Guittierrez, R. F., Griffen, J. E., and Wilson, J. D. Androgen resistance associated with a qualitative abnormality of the androgen receptor and responsive to high dose androgen therapy. J Clin Endocrinol Metab 68:578-585; 1989.
16. Hackenberg, R., Hawighorst, T., Filmer, A., Slater, E. P., Bock, K., Beato, M., and Schulz, K.-D. Regulation of androgen receptor mRNA and protein level by steroid hormones in human mammary cancer cells. J Ster Biochem Mol Biol 43:599-607; 1992.
17. Hall, R. E., Tilley, W. D., McPhaul, M. J., and Sutherland, R. L. Regulation of androgen receptor gene expression by steroids and retinoic acid in human breast-cancer cells. Int J Cancer 52:778-784; 1992.
18. Hirose, T., Koga, M., Saito, H., Kouhara, H., Sumitani, S., Kasayama, S., Matsumoto, K., and Sato, B. Functional abnormality of glucocorticoid receptor in Shionogi carcinoma 115 cells as evidenced by gene transfer experiments. J Ster Biochem Mol Biol 38:593-598; 1991.
19. Isaia, G., Mussetta, M., Pecchio, F., Sciolla, A., di Stefano, M., and Molinatti, G. M. Effect of testosterone on bone in hypogonadal males. Maturitas 15:47-51; 1992.
20. Ishida, Y. and Heersche, J. N. M. Progesterone stimulates proliferation and differentiation of osteoprogenitor cells in bone cell populations derived from adult female but not from adult male rats. Bone 20:17-25; 1997.
21. Ito, Y., Fisher, C. R., Conte, F. A., Grumbach, M. M., and Simpson, E. R. Molecular basis of aromatase deficiency in an adult female with sexual infantilism and polycystic ovaries. Proc Natl Acad Sci USA 90:11673-11677; 1993.
22. Jackson, J. A. and Kleerekoper, M. Osteoporosis in men: Diagnosis, pathophysiology, and prevention. Medicine 69:137-152; 1990.
23. Kasperk, C., Fitzsimmons, R., Strong, D., Mohan, S., Jennings, J., Wergedal, J., and Baylink, D. Studies of the mechanism by which androgens enhance mitogenesis and differentiation in bone cells. J Clin Endocrinol Metab 71: 1322-1329; 1990.
24. Kasperk, C. H., Wergeda, J. E., Farley, J. R., Linkhart, T. A., Turner, R. T., and Baylink, D. J. Androgens directly stimulate proliferation of bone cells in vitro. Endocrinology 124:1576-1579; 1989.
25. Keeting, P. E., Scott, R. E., Colvard, D. S., Anderson, M. A., Oursler, M. J., Spelsberg, T. C., and Riggs, B. L. Development and characterization of a rapidly proliferating, well-differentiated cell line derived from normal adult human osteoblast-like cells transfected with SV40 large T antigen. J Bone Miner Res 7:127-136; 1992.
26. Kemppainen, J. A., Lane, M. V., Sar, M., and Wilson, E. M. Androgen receptor phosphorylation, turnover, nuclear transport, and transcriptional activation. Specificity for steroids and antihormones. J Biol Chem 267:968-974; 1992.
27. Koenig, H., Goldstone, A., and Lu, C. Y. Testosterone induces a rapid stimulation of endocytosis, amino acid and hexose transport in mouse kidney cortex. Biochem Biophys Res Commun 106:346-353; 1982.
28. Kovacs, W. J. and Turney, M. K. High efficiency covalent radiolabeling of the human androgen receptor. Studies in cultured fibroblasts using dihydrotestosterone 17 beta-bromoacetate. J Clin Invest 81:342-348; 1988.
29. Krongrad, A., Wilson, C. M., Wilson, J. D., Allman, D. R., and McPhaul, M. J. Androgen increases androgen receptor protein while decreasing receptor mRNA in LNCaP cells. Mol Cell Endocrinol 76:79-88; 1991.
30. Landers, J. P. and Spelsberg, T. C. Updates and new models for steroid hormone action. Ann NY Acad Sci 626:26-55; 1991.
31. Landers, J. P. and Spelsberg, T. C. New concepts in steroid hormone action: Transcription factors, proto-oncogenes, and the cascade model for steroid regulation of gene expression. Crit Rev Euk Gene Expr 2:19-63; 1992.
32. Lea, C., Kendall, N., and Flanagan, A. M. Casodex (a non-steroidal antiandrogen) reduces cancellous, endosteal, and periosteal bone formation in estrogenreplete female rats. Calcif Tissue Int 58:268-272; 1996.
33. Liang, P. and Pardee, A. B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971; 1992.
34. Lieberherr, M., Grosse, B., Kachkache, M., and Balsan, S. Cell signaling and estrogens in female rat osteoblasts: A possible involvement of unconventional nonnuclear receptors. J Bone Miner Res 8:1365-1376; 1993.
35. Matzkin, H., Chen, J., Weisman, Y., Goldray, D., Pappas, F., Jaccard, N., and Braf, Z. Prolonged treatment with finasteride (a 5 alpha-reductase inhibitor) does not affect bone density and metabolism. Clin Endocrinol (Oxford) 37:432-436; 1992.
36. Masuyama, A., Ouchi, Y., Sato, F., Hosoi, T., Nakamura, T., and Orimo, H. Characteristics of steroid hormone receptors in cultured MC3T3-E1 osteoblastic cells and effect of steroid hormones on cell proliferation. Calcif Tissue Int 51:376-381; 1992.
37. McPhaul, M. J., Deslypere, J. P., Allman, D. R., and Gerard, R. D. The adenovirus-mediated delivery of a reporter gene permits the assessment of androgen receptor function in genital skin fibroblast cultures. J Biol Chem 268:26063-26066; 1993.
38. Mizokami, A. and Chang, C. Induction of translation by the 5′ untranslated region of human androgen receptor mRNA. J Biol Chem 269:25655-25659; 1994.
39. Morishima, A., Grumbach, M. M., Simpson, E. R., Fisher, C., and Qin, K. Aromatase deficiency in male and female siblings caused by a novel mutation and the physiological role of estrogens. J Clin Endocrinol Metab 80:3689-3698; 1995.
40. Munson, P. J. and Rodbard, D. LIGAND: A versatile computerized approach for characterization of ligand-binding systems. Anal Chem 107:220-239; 1980.
41. Nakano, Y., Morimoto, I., Ishida, O., Fujihira, T., Mizokami, A., Tanimoto, A., Yanagihara, N., Izumi, F., and Eto, F. The receptor, metabolism and effects of androgen in osteoblastic MC3T3-E1 cells. Bone Miner 26:245-259; 1994.
42. Orwoll, E. S. Androgens as anabolic agents for bone. Trends Endocrin Metab 7:77-84; 1996.
43. Orwoll, E. S. Androgens. In: Bilezikian, J. P., Raisz, L. G., and Rodan, G. A., Eds. Principles of Bone Biology. Boston, Academic; 1996; 563-580.
44. Orwoll, E. S., Stribrska, L., Ramsey, E. E., and Keenan, E. J. Androgen receptors in osteoblast-like cell lines. Calcif Tissue Int 49:183-187; 1991.
45. Otten, A. D., Sanders, M. M., and McKnight, G. S. The MMTV LTR promoter is induced by progesterone and dihydrotestosterone but not by estrogen. Mol Endocrinol 2:143-147; 1988.
46. Purohit, A., Flanagan, A. M., and Reed, M. J. Estrogen synthesis by osteoblast cell lines. Endocrinology 131:2027-2029; 1992.
47. Rao, L. G., Wylie, J. N., Sutherland, M. S., and Murray, T. M. 17 Beta-estradiol and parathyroid hormone potentiate each other's stimulatory effects on alkaline phosphatase activity in SaOS-2 cells in a differentiation-dependent manner. Endocrinology 134:614-620; 1994.
48. Rosner, W., Hryb, D. J., Khan, M. S., Nakhla, A. M., and Romas, N. A. Sex hormone-binding globulin: Anatomy and physiology of a new regulatory system. J Ster Biochem Mol Biol 40:813-820; 1991.
49. Roy, A. K., Chatterjee, B., Rao, K. V., Murty, C. V., Sarkar, F. H., and Majumdar, D. Androgenic regulation of hepatic gene expression. J Ster Biochem Mol Biol 27:1129-1134; 1987.
50. Sambrook, J., Fritsch, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Press; 1989.
51. Schüle, R., Rangarajan, P., Kliewer, S., Ransone, L. J., Bolado, J., Yang, N., Verma, I. M., and Evans, R. M. Functional antagonism between oncoprotein c-Jun and the glucocorticoid receptor. Cell 62:1217-1226; 1990.
52. Shemshedini, L., Knauthe, R., Sassone-Corsi, P., Pornon, A., and Gronemeyer, H. Cell-specific inhibitory and stimulatory effects of Fos and Jun on transcription activation by nuclear receptors. EMBO J 10:3839-3849; 1991.
53. Silberberg, M. and Silberberg, R. Further investigations on the effect of the male sex hormone on endochondral ossification. Anat Rec 95:97-117; 1964.
54. Silberberg, M. and Silberberg, R. Steroid hormones and bone. In: Bourne G. H., Ed. The Biochemistry and Physiology of Bone, 2nd ed. New York: Academic; 1971; 401-484.
55. Smith, E. P., Boyd, J. G. R. F., Takahashi, H., Cohen, R. M., Specker, B., Williams, T. C., Lubahn, D. B., and Korach, K. S. Estrogen resistance caused by a mutation in the estrogen-receptor gene in a man. New Engl J Med 331:1056-1061; 1994.
56. Somjen, D., Mor, Z., and Kaye, A. M. Age dependence and modulation by gonadectomy of the sex-specific response of rat diaphyseal bone to gonadal steroids. Endocrinology 134:809-814; 1994.
57. Stebbings, W. S. L., Anderson, E., Puddefoot, J. R., Farthing, M. J. G., and Vinson, G. P. A hybrid ligand method for androgen receptor measurement. J Ster Biochem Mol Biol 31:181-186; 1988.
58. Stein, G. S., Lian, J. B., and Owen, T. A. Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEBJ 4:3111-3123; 1990.
59. Supakar, P. C., Song, C. S., Jung, M. H., Slomczynska, M. A., Kim, J. M., Vellanoweth, R. L., Chatterjee, B., and Roy, A. K. A novel regulatory element associated with age-dependent expression of the rat androgen receptor gene. J Biol Chem 268:26400-26408; 1993.
60. Swerdloff, R. S. and Wang C. Androgens and aging in men. Exp Gerontol 28:435-446; 1993.
61. Takeuchi, M., Kakushi, H., and Tohkin, M. Androgens directly stimulate mineralization and increase androgen receptors in human osteoblast-like osteosarcoma cells. Biochem Biophys Res Commun 204:905-911; 1994.
62. Tanaka, S., Haji, M., Nishi, Y., Yanase, T., Takayanagi, R., and Nawata, H. Aromatase activity in human osteoblast-like osteosarcoma cell. Calcif Tissue Int 52:107-109; 1993.
63. Trifiro, M., Gottlieb, B., Pinsky, L., Kaufman, L., Kaufman, M., Prior, L., Belsham, D. D., Wrogemann, K., Brown, C. J., Willard, H. P., Trapman, J., Brinkmann, A. O., Chang, C., Liao, S., Sergovich, F., and Jung, J. The 56/58 kDa androgen-binding protein in male genital skin fibroblasts with a deleted androgen receptor gene. Mol Cell Endocrinol 75:37-47; 1991.
64. Van Gelderen, H. H. Skeletal maturation in the XY female syndrome. Clin Genet 30:199-201; 1986.
65. Vanderschueren, D., Van Herck, E., Suiker, A. M., Visser, W. J., Schot, L. P., Chung, K., Lucas, R. S., Einhorn, T. A., and Bouillon, R. Bone and mineral metabolism in the androgen-resistant (testicular feminized) male rat. J Bone Miner Res 8:801-809; 1993.
66. Varrela, J., Alvesalo, L., and Vinkka, H. Body size and shape in 45,XY females with complete testieular feminization. Ann Hum Biol 11:291-301; 1984.
67. Vegeto, E., Shahbaz, M. M., Wen, D. X., Goldman, M. E., O'Malley, B. W., and McDonnell, D. P. Human progesterone receptor A form is a cell-and promoter-specific repressor of human progesterone receptor B function. Mol Endocrinol 7:1244-1255; 1993.
68. Weisman, Y., Cassorla, F., Malozowski, S., Krieg, R. J. J., Goldray, D., Kaye, A. M., and Somjen, D. Sex-specific response of bone cells to gonadal steroids: Modulation in perinatally androgenized females and in testicular feminized male rats. Steroids 58:126-133; 1993.
69. Wilson, C. M. and McPhaul, M. J. A and B forms of the androgen receptor are present in human genital skin fibroblasts. Proc Natl Acad Sci USA 91:1234-1238: 1994.
70. Wolf, D. A., Herzinger, T., Hermeking, H., Blaschke, D., and Horz, W. Transcriptional and posttranscriptional regulation of human androgen receptor expression by androgen. Mol Endocrinol 7:924-936; 1993.
71. Yang-Yen, H.-F., Chambard, J.-C., Yu-Lin, S., Smeal, T., Schmidt, T. J., Drouin, J., and Karin, M. Transcriptional interference between c-Jun and the glucocorticoid receptor: Mutual inhibition of DNA binding due to direct protein-protein interaction. Cell 62:1205-1215; 1990.
72. Zhou, Z.-x., Lane, M. V., Kemppainen, J. A., French, F. S., and Wilson, E. M. Specificity of ligand-dependent androgen receptor stabilization: Receptor domain interactions influence ligand dissociation and receptor stability. Mol Endocrinol 9:208-218; 1995.