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Volumn 7, Issue 15, 1997, Pages 2049-2054

Evidence for induced DNA bending by the yeast zinc cluster protein PUT3

Author keywords

[No Author keywords available]

Indexed keywords

CURVED DNA; DNA BINDING PROTEIN; TRANSCRIPTION FACTOR;

EID: 0030877054     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00355-7     Document Type: Article
Times cited : (2)

References (23)
  • 17
    • 0024814234 scopus 로고
    • between the unique Xba I and Sal I restriction sites to generate plasmids pPH-PUT-26, 28, 30, 33 and 35, respectively. Recombinant plasmids were identified after transformation into competent E. coli GM2929 cells by screening for loss of the Sal I site. Plasmids were then sequenced by the Sanger dideoxy-mediated chain-termination method. Digestion of the plasmids with Stu I gave the a set of phasing fragments which were purified by agarose gel electrophoresis, amplified by PCR, and labeled on the 5′-end
    • 10 site (in bold) and three phased A-tracts (in italics) were cloned into pBEND2[Kim, J.; Zwieb, C.; Wu, C.; Adhya, S., Gene 1989, 85, 15-23] between the unique Xba I and Sal I restriction sites to generate plasmids pPH-PUT-26, 28, 30, 33 and 35, respectively. Recombinant plasmids were identified after transformation into competent E. coli GM2929 cells by screening for loss of the Sal I site. Plasmids were then sequenced by the Sanger dideoxy-mediated chain-termination method. Digestion of the plasmids with Stu I gave the a set of phasing fragments which were purified by agarose gel electrophoresis, amplified by PCR, and labeled on the 5′-end.
    • (1989) Gene , vol.85 , pp. 15-23
    • Kim, J.1    Zwieb, C.2    Wu, C.3    Adhya, S.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.