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between the unique Xba I and Sal I restriction sites to generate plasmids pPH-PUT-26, 28, 30, 33 and 35, respectively. Recombinant plasmids were identified after transformation into competent E. coli GM2929 cells by screening for loss of the Sal I site. Plasmids were then sequenced by the Sanger dideoxy-mediated chain-termination method. Digestion of the plasmids with Stu I gave the a set of phasing fragments which were purified by agarose gel electrophoresis, amplified by PCR, and labeled on the 5′-end
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