Rapid assay of intrinsic radiosensitivity based on apoptosis in human CD4 and CD8 T-lymphocytes

International Journal of Radiation Oncology Biology Physics

Volumn 38, Issue 2, 1997, Pages 429-440

  Ozsahin, Mahmut ^a,d   Ozsahin, Hülya ^b   Shi, Yuquan ^a   Larsson, Boerje ^a   Würgler, Friedrich E ^a   Crompton, Nigel E A ^a,c  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b UNIVERSITY CHILDREN S HOSPITAL   (Switzerland)

^c PAUL SCHERRER INSTITUTE   (Switzerland)

^d LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

Apoptosis; Ataxia telangiectasia (AT); Centomeric instability; Facial Anomalies Syndrome (ICF); Flow cytometry; Immunodeficiency; Lymphocyte; Radiosensitivity

Indexed keywords

CD4 ANTIGEN; CD8 ANTIGEN; DNA;

ADULT; APOPTOSIS; ARTICLE; CHILD; CONTROLLED STUDY; CYTOTOXICITY; DNA DEGRADATION; FEMALE; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; RADIATION RESPONSE; RADIOSENSITIVITY; T LYMPHOCYTE SUBPOPULATION;

ADULT; APOPTOSIS; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CHILD; CHILD, PRESCHOOL; FEMALE; HUMANS; MALE; RADIATION TOLERANCE;

EID: 0030872550     PISSN: 03603016     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0360-3016(97)00038-2     Document Type: Article

References (44)

1. Ayukawa, K.; Tomooka, S.; Asano, T.; Taniguchi, K.; Yoshikai, Y.; Nomoto, K. Radioresistant CD4-CD8+ intrathymic T cell precursors differentiate into mature CD4+CD8-and CD4-CD8+ T cells. Thymus 15:65-78; 1990.
2. Begg, A. C.; Russell, N. S.; Knaken, H.; Lebesque, J. V. Lack of correlation of human fibroblast radiosensitivity in vitro with early skin reactions in patients undergoing radiotherapy. Int. J. Radiat. Biol. 64:393-405; 1993.
3. Brown, D. C.; Grace, E.; Sumner, A. T.; Edmunds, A. T.; Ellis, P. M. ICF syndrome (immunodeficiency, centromeric instability and facial anomalies): Investigation of heterochromatin abnormalities and review of clinical outcome. Hum. Genet. 96:411-416; 1995.
4. Carter, P. H.; Resto-Ruiz, S.; Washington, G. C.; Ethridge, S.; Palini, A.; Vogt, R.; Waxdal, M.; Fleisher, T.; Noguchi, P. D.; Marti, G. E. Flow cytometric analysis of whole blood lysis, three anticoagulants, and five cell preparations. Cytometry 13:68-74; 1992.
5. Crompton, N. E. A.; Ozsahin, M. A versatile and rapid radiosensitivity assay of peripheral blood leukocytes based on DNA and surface marker assessment of cytotoxicity. Radiat. Res. 147:55-60; 1997.
6. Darzynkiewicz, Z.; Bruno, S.; Del Bino, G.; Gorczyca, W.; Hotz, M. A.; Lassota, P.; Traganos, F. Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808; 1992.
7. Dehos, A.; Kriehuber, R.; Czempiel, H.; Baumgartner, I.; Egblomasse, C.; Burkart, W. Changes in subpopulations of lymphocytes after exposure to ionizing radiation in vitro and in vivo. In: Huilgol N. G., Gopinath, D. V., Singh, B. B., eds. Low level radiation and living state. Berlin: Springer Verlag; 1994:102-108.
8. Duchaud, E.; Ridet, A.; Délic, Y.; Cundari, E.; Moustacchi, E.; Rosselli, F. Altération de la réponse apoptotique radio-induite chez des homozygotes et des hétérozygotes pour l'ataxie-télangiectasie. Comptes Rendus Acad. Sci. III 317:983-989; 1994.
9. Dutkowski, R. T.; Lesh, R.; Staiana-Coico, L.; Thaler, H.; Darlington, G. J.; Weksler, M. E. Increased chromosomal instability in lymphocytes from elderly humans. Mutat. Res. 149:505-512; 1985.
10. Dutreix, J.; Girinsky, T.; Cosset, J. M.; Bernard, A.; Pico, J.; Baume, D.; Bayle, C.; Benk, V. Blood cell kinetics and total body irradiation. Radiother. Oncol. 9:119-129; 1987.
11. Elyan, S. A. G.; West, C. M. L.; Roberts, S. A.; Hunter, R. D. Use of low-dose rate irradiation to measure the intrinsic radiosensitivity of human T-lymphocytes. Int. J. Radiat. Biol. 64:375-383; 1993.
12. Elyan, S, A. G.; West, C. M. L.; Roberts, S. A.; Hunter, R. D. Use of an internal standard in comparative measurements of the intrinsic radiosensitivities of human T-lymphocytes. Int. J. Radiat. Biol. 64:385-391; 1993.
13. Geara, F. B.; Peters, L. J.; Ang, K. K.; Wike, J. L.; Sivon, S. S.; Guttenberger, R.; Callender, D. L.; Malaise, E. P.; Brock, WA. Intrinsic radiosensitivity of normal human fibroblasts and lymphocytes after high-and low-dose-rate irradiation. Cancer Res. 52:6348-6352; 1992.
14. Girinsky, T.; Socié, G.; Cosset, J. M.; Malaise, E. P. Blood lymphocyte subsets after the first fraction in patients given hyperfractionated total body irradiation for bone marrow transplantation. Br. J. Cancer 63:646-647; 1991.
15. Green, M. H. L.; Arlett, C. F.; Cole, J.; Harcourt, S. A.; Priestly, A.; Waugh, A. P. W.; Stephens, G.; Beare, D. M.; Brown, N. A. P.; Shun-Shin, G. A. Comparative human cellular radiosensitivity: III. γ-radiation survival of cultured skin fibroblasts and resting T lymphocytes from the peripheral blood of the same individual. Int. J. Radiat. Biol. 59:749-765; 1991.
16. Hart, R. M.; Kimler, B. F.; Evans, G. R.; Park, C. H. Radiotherapeutic management of medulloblastoma in a pediatric patient with ataxia telangiectasia. Int. J. Radiat. Oncol. Biol. Phys. 13:1237-1240; 1988.
17. Jones, L. A.; Scott, D.; Cowan, R.; Roberts, S. A. Abnormal radiosensitivity of lymphocytes from breast cancer patients with excessive normal tissue damage after radiotherapy: Chromosome aberrations after low dose-rate irradiation. Int. J. Radiat. Biol. 67:519-528; 1995.
18. Kushirio, J. I.; Nakamura, N.; Kyoizumi, S.; Nishiki, M.; Dohi, K.; Ayiyama, M. Absence of correlations between radiosensitivities of human T-lymphocytes in GO and skin fibroblasts in log phase. Radiat. Res. 122:326-332; 1990.
19. Lewis, P. D. Variation in individual sensitivity to ionizing radiation. In: Russell Jones, R.; Southwood, R., eds. Radiation and health. Chichester, UK: Wiley; 1987:167-177.
20. Lyons, A. B.; Samuel, K.; Sanderson, A.; Maddy, A. H. Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry. Cytometry 13:809-821; 1992.
21. Meyn, M. S. Ataxia-telangiectasia and cellular responses to DNA damage. Cancer Res. 55:5991-6001; 1995.
22. Nakamura, N.; Kusunoki, Y.; Akiyama, M. Radiosensitivity of CD4 or CD8 positive human T-lymphocytes by an in vitro colony formation assay. Radiat. Res. 123:224-227; 1990.
23. Nakamura, N.; Sposto, R.; Kushiro, J. I.; Akiyama, M. Is interindividual variation of cellular radiosensitivity real or artificial? Radiat. Res. 125:326-330; 1991.
24. Norman, A.; Kagan, A. R.; Chan, S. L. The importance of genetics for the optimization of radiation therapy. Am. J. Clin. Oncol. 11:84-88; 1988.
25. Peters, L. J. Significance of genetic variability in radiosen-sitivity in clinical radiotherapy. J. Jpn. Soc. Ther. Radiol. Oncol. 2:247-253; 1990.
26. Radford, I. R.; Murphy, T. K. Radiation response of mouse lymphoid and myeloid cell lines. Part III. Different signals can lead to apoptosis and may influence sensitivity to killing by DNA double-strand breakage. Int. J. Radiat. Biol. 65:229-239; 1994.
27. Radford, I. R.; Murphy, T. K.; Radley, J. M.; Ellis, S. L. Radiation response of mouse lymphoid and myeloid cell lines. Part II. Apoptotic death is shown by all lines examined. Int. J. Radiat. Biol. 65:217-227; 1994.
28. Rosselli, F.; Ridet, A.; Soussi, T.; Duchaud, E.; Alapetite, C.; Moustacchi, E. p53-dependent pathway of radio-induced apoptosis is altered in Fanconi anemia. Oncogene 10:9-17; 1995.
29. Savitsky, K.; Bar-Shira, A.; Gilad, S.; Rotman, G.; Ziv, Y.; Vanagaite, L.; Tagle, D. A.; Smith, S.; Uziel, T.; Sfez, S.; Ashkenazi, M.; Pecker, I.; Frydman, M.; Harnik, R.; Patanjali, S. R.; Simmons, A.; Clines, G. A.; Sartiel, A.; Gatti, R. A.; Chessa, L.; Sanal, O.; Lavin, M. F.; Jaspers, N. G. J.; Taylor, A. M. R.; Arlett, C. F.; Miki, T.; Weissman, S. M.; Lovett, M.; Collins, F. S.; Shiloh, Y. A single ataxia telangiectasia gene with a product similar to PI-3 kinase. Nature 268:1749-1753; 1995.
30. Seki, H.; Iwai, K.; Kanegane, H.; Konno, A.; Ohta, K.; Ohta, K.; Yachie, A.; Taniguchi, N.; Miyawaki, T. Differential protective action of peripheral lymphocyte subpopulations. Cell Immunol. 163:30-36; 1995.
31. Stewart, C. C.; Stevenson, A. P.; Habbersett, R. C. The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets. Int. J. Radiat. Biol. 53:77-87; 1988.
32. Taylor, A. M.; Harnden, D. G.; Arlett, C. F.; Harcourt, S. A.; Lehman, A. R.; Stevens, S.; Bridges, B. A. Ataxia telangiectasia: A human mutation with abnormal radiation sensitivity. Nature 258:427-429; 1975.
33. Telford, W. G.; King, L. E.; Fraker, P. J. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13:137-143; 1992.
34. Terstappen, L. W. M. M.; Loken, M. R. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry 9:548-556; 1988.
35. Toba, K.; Winton, E. F.; Bray, R. A. Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation. Cytometry 13:60-67; 1992.
36. Trowell, O. A. The sensitivity of lymphocytes to ionising radiation. J. Pathol. Bacteriol. 64:687-704; 1952.
37. Trowell, O. A.; Corp, M. J.; Lush, W. R. Paradoxical resistance of thymus lymphocytes to high doses of X-radiation. Radiat. Res. 7:120-128; 1957.
38. UNSCEAR. Effects on the immune system. United Nations Scientific Committee on the Effects of Atomic Radiation. Report to the General Assembly, with Annexes. Annexe B. New York, NY: United Nations; 1994:206-214.
39. Waugh, A. P. W.; Beare, D. M.; Arlett, C. F.; Green, M. H. L.; Cole, J. Comparative human cellular radiosensitivity: IV, The increased sensitivity of human neonatal cord blood lymphocytes to γ-irradiation compared with lymphocytes from children and adults. Int. J. Radiat. Biol. 59:767-776; 1991.
40. West, C. M. L.; Davidson, S. E.; Burt, P. A.; Hunter, R. D. The intrinsic radiosensitivity of cervical carcinoma: Correlations with clinical data. Int. J. Radiat. Oncol. Biol. Phys. 31:841-846; 1995.
41. West, C. M. L.; Hendry, J. H. Intrinsic radiosensitivity as a predictor of patient response to radiotherapy. Br. J. Radiol. 24(Suppl.):146-152; 1992.
42. West, C. M. L.; Hendry, J. H.; Scott, D.; Davidson, S. E.; Hunter, R. D. 25th Paterson Symposium: Is there a future for radiosensitivity testing? Br. J. Cancer 64:197-199; 1991.
43. West, C. M. L.; Scott, D.; Peacock, J. H. Normal cell radiosensitivity: Clinical application in predicting response to radiotherapy and cancer predisposition. Int. J. Radiat. Biol. 66:231-234; 1994.
44. Zamai, L.; Falcieri, E.; Zauli, G.; Cataldi, A.; Vitale, M. Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 14:891-897; 1993.