A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity

Radiation Research

Volumn 147, Issue 1, 1997, Pages 55-60

  Crompton, Nigel E A ^a   Ozsahin, Mahmut ^a,b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE MARKER;

ARTICLE; CELL CYCLE G0 PHASE; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; FEMALE; HUMAN; HUMAN CELL; LEUKOCYTE; MALE; PRIORITY JOURNAL; RADIATION DOSE; RADIATION INJURY; RADIOSENSITIVITY; T LYMPHOCYTE;

ADULT; AGED; ANTIGENS, CD; B-LYMPHOCYTES; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL CYCLE; CELL SURVIVAL; DNA; DOSE-RESPONSE RELATIONSHIP, RADIATION; FEMALE; FLOW CYTOMETRY; G0 PHASE; G1 PHASE; GRANULOCYTES; HUMANS; KILLER CELLS, NATURAL; LEUKOCYTES; MALE; MIDDLE AGED; MONOCYTES; X-RAYS;

EID: 0031021845     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.2307/3579443     Document Type: Article

References (12)

1. I. R. Radford, T. K. Murphy, J. M. Radley and S. L. Ellis, Radiation response of mouse lymphoid and myeloid cell lines. Part II. Apoptotic death is shown by all lines examined. Int. J. Radiat. Biol. 65,217-227 (1994).
2. United Nations Scientific Committee on the Effects of Atomic Radiation, Effects on the immune system. In Report to the General Assembly, Annex B, pp. 206-214. United Nations, New York, 1994.
3. Z. Darzynkiewicz, S. Bruno, G. Del Bino, W. Gorczyca, M. A. Hotz, P. Lassota and F. Traganos, Features of apoptotic cells measured by flow cytometry. Cytometry 13,795-808 (1992).
4. L. Zamai, E. Falcieri, G. Zauli, A. Cataldi and M. Vitale, Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 14,891-897 (1993).
5. S. A. G. Elyan, C. M. L. West, S. A. Roberts and R. D. Hunter, Use of low-dose rate irradiation to measure the intrinsic radiosensitivity of human T-lymphocytes. Int. J. Radiat. Biol. 64,375-383 (1993).
6. N. E. A. Crompton, M. Ozsahin, L. Melicharova and B. Larsson, A rapid assay of patient radiosensitivity. In Proceedings of the Journées Scientifiques de la Société Suisse de Radiobiologie et de Physique Médicale (J. F. Germond and J. M. Haefiiger, Eds.), pp. 157-162. Heng SA, La Chaux-de-Fonds, 1995.
7. C. C. Stewart, A. P. Stevenson and R. C. Habbersett, The effect of low-dose irradiation on unstimulated and PHA-stimulated human lymphocyte subsets. Int. J. Radiat. Biol. 53,77-87 (1988).
8. R. Rana, M. Vitale, G. Mazzotti, L. Manzoli and S. Papa, Radiosensitivity of human natural killer cells: Binding and cytotoxic activities of natural killer cell subsets. Radiat. Res. 124,96-102 (1990).
9. + T cells. Thymus 15,65-78 (1990).
10. H. Seki, K. Iwai, H. Kanegane, A. Konno, K. Ohta, A. Yachie, N. Taniguchi and T, Miyawaki, Differential protective action of peripheral lymphocyte subpopulations. Cell Immunol. 163,30-36 (1995).
11. N. Nakamura, Y. Kusunoki and M. Akiyama, Radiosensitivity of CD4 or CDS positive human T-lymphocytes by an in vitro colony formation assay. Radiat. Res, 123,224-227 (1990).
12. W. G. Telford, L. E. King and J. P. Fraker, Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13,137-143 (1992).