Hierarchical affinity maturation of a phage library derived antibody for the selective removal of cytomegalovirus from plasma

Journal of Immunological Methods

Volumn 206, Issue 1-2, 1997, Pages 171-182

  Pini, Alessandro ^a   Spreafico, Adriano ^a   Botti, Roberta ^a   Neri, Dario ^b   Neri, Paolo ^a  

^a UNIVERSITY OF SIENA   (Italy)

^b ETH ZURICH   (Switzerland)

Author keywords

Affinity maturation; Antibody phage library; BIAcore; Combinatorial mutagenesis; Immunoaffinity separation; Single chain Fv

Indexed keywords

ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; ARTICLE; BACTERIOPHAGE; BINDING AFFINITY; CYTOMEGALOVIRUS INFECTION; NONHUMAN; PRIORITY JOURNAL;

ANTIBODIES, VIRAL; ANTIBODY AFFINITY; BASE SEQUENCE; BIOTECHNOLOGY; CLONING, MOLECULAR; CYTOMEGALOVIRUS; FILTRATION; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; INOVIRUS; MOLECULAR SEQUENCE DATA; PLASMA; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5;

EID: 0030870758     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00107-5     Document Type: Article

References (36)

1. Azimzadeh, A., Weiss, E., Van Regenmortel, M.H.V., 1992a. Measurement of affinity of viral monoclonal antibodies using FAB'-peroxidase conjugate. Influence of antibody concentration on apparent affinity. Mol. Immunol. 29, 601-608.
2. Azimzadeh, A., Pellequer, J.L., Van Regenmortel, M.H.V., 1992b. Operational aspects of antibody affinity constants measured by liquid phase and solid phase assays. J. Mol. Recog. 5, 9-18.
3. Barbas, C.F. III, Kang, A.S., Lerner, R.A., Benkovic, S.J., 1991. Assembly of combinatorial antibody libraries on phage surface: The gene III site. Proc. Natl. Acad Sci. USA 88, 7978-7982.
4. Barbas, C.F. III, Hu, D., Dunlop, N., Sawyer, L., Cabada, D., Hendry, R.M., Nara, P.L., Burton, D.R., 1994. In vitro evolution of a neutralizing human antibody to human immunodeficiency virus type 1 to enhance affinity and broaden strain cross-reactivity. Proc. Natl. Acad. Sci USA 91, 3809-3813.
5. Begent, R.H.J., Chester, K.A., Connors, T., Crowther, D., Fox, B., Griffiths, E., Hince, T.A., Ledermann, J.A., McVie, J.G., Minor, P., Secher, D.S., Schwartsmann, G., Thorpe, R., Wilbin, C., Zwierzina, H., 1993. Cancer research campaign operation manual for control recommendations for products derived from recombinant DNA technology prepared for investigational administration to patients with cancer in phase I trials. Eur. J. Cancer 29, 1907-1910.
6. Bird, R.E., Hardman, K.D., Jacobson, J.W., Kaufman, B.M., Lee, S.M., Lee, T., Pope, S.H., Rioardan, G.S., Whitlow, M., 1988. Single chain antigen-binding proteins. Science 242, 423-426.
7. Caanan-Haden, L., Fernandez-de-Cossio, M.E., Pedroso, I., Rodes, L., Gavilondo, J.V., 1995. Purification and application of a single-chain Fv antibody fragment specific to hepatitis B virus surface antigen. Biotechniques 19, 606-614.
8. Carnemolla, B., Neri, D., Castellani, P., Leprini, A., Neri, G., Pini, A., Winter, G., Zardi, L., 1996. Phage antibodies with pan-species recognition of the oncofetal angiogenesis marker fibronectin ED-B domain. Int. J. Cancer 68, 397-405.
9. Champluvier, B., Kula, M.R., 1991. Microfiltration membranes as pseudo affinity adsorbents: Modification and comparison with gel beads. J. Chromatogr. 539, 315-325.
10. Crothers, D.M., Metzger, H., 1972. The influence of polyvalency on the binding properties of antibodies. Immunochemistry 9, 341-357.
11. de Kruif, J., Boel, E., Logtemberg, T., 1995. Selection and application of human single chain Fv antibody fragments from a semi-synthetic phage antibody display library with designed CDR3 regions. J. Mol. Biol. 248, 97-105.
12. Friguet, B., Chaffotte, A.F., Djavadi-Ohaniance, L., Goldberg, M.E., 1985. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 77, 305-319.
13. Greenfield, N., Fasman, G., 1969. Computed circular dichroism spectra for the evaluation of protein conformation. Biochemistry 8, 4108-4116.
14. Griffiths, A.D., Williams, S.C., Hartly, O., Tomlison, I.M., Waterhouse, P., Crosby, W.L., Kontermann, R.E., Jones, P.T., Low, N.M., Allison, T.J., Prospero, T.D., Hoogenboom, H.R., Nissim, A., Cox, J.P.L., Harrison, J.L., Zaccolo, M., Gherardi, E., Winter, G., 1994. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260.
15. Hoogenboom, H.R., Griffiths, A.D., Johnson, K.S., Chiswell, D.J., Hudson, P., Winter, G., 1991. Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19, 4133-4137.
16. Hoogenboom, H.R., Winter, G., 1992. By-passing immunization. Human antibodies from synthetic repertoires of germline VH gene segments rearranged in vitro. J. Mol. Biol. 227, 381-388.
17. Hopp, T.P., Prickett, K.S., Price, V., Libby, R.T., March, C.J., Cerretti, P., Urdal, D.L., Conlon, P.J., 1988. A short polypeptide marker sequence useful for recombinant protein identification and purification. Biotechnology 6, 1205-1210.
18. Huston, J.S., Levinson, D., Mudgett, H.M., Tai, M.S., Novotny, J., Margolies, M.N., Ridge, R.J., Bruccoleri, R.E., Haber, E., Crea, R., Oppermann, H., 1988. Protein engineering of antibody binding sites: Recovery of specific activity in an antidigoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. USA 85, 5879-5883.
19. Jönsson, U., Fägerstam, L., Ivarsson, B., Johnsson, B., Karlsson, R., Lundh, K., Löfås, S., Persson, B., Roos, H., Rönnberg, I., Sjölander, S., Stenberg, E., Ståhlberg, R., Urbaniczky, C., Östlin, H., Malmqvist, M., 1991. Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. Biotechniques 11, 620-627.
20. Marks, J.D., Hoogenboom, H.R., Bonnert, T.P., McCafferty, J., Griffiths, A.D., Winter, G., 1991. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
21. McCafferty, J., Griffiths, A.D., Winter, G., Chiswell, D.J., 1990. Phage antibodies: Filamentous phage displaying antibody variable domains. Nature 348, 552-554.
22. Molloy, P., Brydon, L., Porter, A.J., Harri, W.J., 1995. Separation and concentration of bacteria with immobilized antibody fragments. J. Appl. Bacteriol. 78, 359-365.
23. Neri, D., Momo, M., Prospero, T., Winter, G., 1995. High-affinity antigen binding by chelating recombinant antibodies (CRAbs). J. Mol. Biol. 246, 367-373.
24. Neri, D., Petrul, H., Light, Y., Marais, R., Britton, K.E., Winter, G., Creighton, A.M., 1996a. Radioactive labelling of recombinant antibody fragments by phosphorylation using human caseine kinase II and (g32-P)-ATP. Nat. Biotechnol. 14, 385-390.
25. Neri, D., Montigiani, S., Kirkham, P.M., 1996b. Biophysical methods for the determination of antibody-antigen affinities. Trends Biotechnol. 14, 465-470.
26. Neri, D., Carnemolla, B., Nissim, A., Pini, A., Tarli, L., Neri, P., Balza, E., Leprini, A., Querze', G., Zardi, L., Winter, G., 1997. Tumor targeting by affinity-matured recombinant antibodies against an angiogenesis associated fibronectin isoform. Submitted.
27. Nissim, A., Hoogenboom, H.R., Tomlison, I.M., Flynn, G., Midgley, C., Lane, D., Winter, G., 1994. Antibody fragments from a single pot phage display library as immunochemical reagents. EMBO J. 13, 692-698.
28. Pack, P., Kujau, M., Schroeckh, U., Knüpfer, R., Wenderoth, R., Riesenberg, D., Plückthun, A., 1993. Improved bivalent mini-antibodies with identical avidity as whole antibodies produced by high cell density fermentation of Escherichia coli. Bio/Technology 11, 1271-1277.
29. Padlan, E.A., 1994. Anatomy of the antibody molecule. Mol. Immunol. 31, 169-217.
30. Scatchard, G., 1949. The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 51, 660.
31. Smith, G.P., 1985. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315-1317.
32. Smith-Gill, S.J., Lavoie, T.B., Mainhart, C.R., 1984. Antigenic regions defined by monoclonal antibodies correspond to structural domains of avian lysozyme. J. Immunol. 133, 384-393.
33. Spector, S.A., Merril, L., Wolf, D., Dankner, W.M., 1992. Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. J. Clin. Microbiol. 30, 2359-2365.
34. Vallar, L., Rivat, C., 1996. Regenerated cellulose-based hemodialyzers with immobilized proteins as potential devices for extracorporeal immunoadsorption procedures: An assessment of protein coupling capacity and in vitro dialysis performance. Artif. Organs 20, 8-16.
35. Vaughan, T.J., Williams, A.J., Pritchard, K., Osbourn, J.K., Pope, A.R., Earnshaw, J.C., McCafferty, J., Hodits, R.A., Wilton, J., Johnson, K.S., 1996. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14, 309-314.
36. Winter, G., Griffiths, A.D., Hawkins, R.E., Hoogenboom, H.R., 1994. Making antibodies by phage display technology. Annu. Rev. Immunol. 12, 433-455.