Purification and characterization of naturally soluble neuropathy target esterase from chicken sciatic nerve by HPLC and western blot

Journal of Neurochemistry

Volumn 69, Issue 5, 1997, Pages 1975-1982

  Escudero, M A ^a   Vilanova, E ^a  

^a UNIVERSITY OF ALICANTE   (Spain)

Author keywords

Neuropathy target esterase; Organophosphorus; Sciatic nerve; Soluble esterase

Indexed keywords

MIPAFOX; NEUROTOXIC ESTERASE; ORGANOPHOSPHORUS COMPOUND;

ANIMAL TISSUE; ARTICLE; CHICKEN; CONTROLLED STUDY; ENZYME LOCALIZATION; ENZYME MODIFICATION; ENZYME PURIFICATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOBLOTTING; NEUROPATHY; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL;

ANIMALIA; GALLUS GALLUS;

EID: 0030870227     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.69051975.x     Document Type: Article

References (31)

1. Aldridge W. N. (1993) The esterases: perspectives and problems. Chem. Biol. Interact. 87, 5-13.
2. Barril J. B., Vilanova E., and Pellin M. C. (1988) Sciatic nerve neuropathy target esterase. Methods of assay, proximodistal distribution and regeneration. Toxicology 49, 107-114.
3. Carrera V., Barril J., Mauricio M., Pellin M. C., and Vilanova E. (1992) Local application of neuropathic organophosphorus compounds to hen sciatic nerve: inhibition of neuropathy target esterase and peripheral neurological impairments. Toxicol. Appl. Pharmacol. 75, 350-357.
4. Chemnitius J. M. and Zech R. (1983) Inhibition of brain carboxyl esterases by neurotoxic organophosphorus compounds. Mol. Pharmacol. 23, 717-723.
5. Escudero M. A., Barril J., Tormo N., and Vilanova E. (1995) Separation of two forms of neuropathy target esterase in the soluble fraction of the hen sciatic nerve. Chem. Biol. Interact. 97, 247-255.
6. Escudero M. A., Sogorb M. A., and Vilanova E. (1996) An automatable microassay for phenyl valerate esterase activities sensitive to organophosphorus compounds. Toxicol. Lett. 89, 241-247.
7. Escudero M. A., Céspedes V., and Vilanova E. (1997) Chromatographic discrimination of soluble neuropathy target esterase isoenzymes and related phenyl valerate esterases from chicken brain, spinal cord, and sciatic nerve. J. Neurochem. 68, 2170-2176.
8. Glynn P. (1996) Neuropathy target esterase (NTE): molecular characterization and cellular localization. Arch. Toxicol. S19, 325-329.
9. 3H]DFP-labelled polypeptide. Chem. Biol. Int. 87, 361-367.
10. Glynn P., Read D. J., Guo R., and Johnson M. K. (1994) Synthesis and characterization of a biotinylated organophosphorus ester for detection and affinity purification of a brain serine esterase: neuropathy target esterase. Biochem. J. 301, 551-556.
11. Hames B. D. (1990) Protein staining with organic dyes and quantitation, in Gel Electrophoresis of Proteins. A Practical Approach (Hames B. D. and Rickwood D., eds), pp. 52-60. Oxford University Press, New York.
12. Johnson M. K. (1969) A phosphorylation site in brain and delayed neurotoxic effects of some organophosphorus compounds. Biochem. J. 111, 487-495.
13. Johnson M. K. (1977) Improved assay of neurotoxic esterase for screening organophosphate for delayed neurotoxicity potential. Arch. Toxicol. 37, 113-115.
14. Johnson M. K. (1982) The target for initiation of delayed neurotoxicity by organophosphorus esters: biochemical and toxicological applications. Rev. Biochem. Toxicol. 4, 141-212.
15. Johnson M. K. (1987) Receptor or enzyme: the puzzle of NTE and organophosphate-induced delayed polyneuropathy. Trends Pharmacol. Sci. 8, 174-179.
16. Johnson M. K. and Glynn P. (1995) Neuropathy target esterase (NTE) and organophosphorus-induced delayed polyneuropathy (OPIDP): recent advances. Toxicol. Lett. 82/83, 459-463.
17. Jokanovic M. and Johnson M. K. (1993) Interactions in vitro of some organophosphoramidates with neuropathy target esterase and acetylcholinesterase of hen brain. J. Biochem. Toxicol. 8, 19-31.
18. Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
19. Lotti M. (1992) The pathogenesis of organophosphate polyneuropathy. Crit. Rev. Toxicol. 21, 465-488.
20. Lotti M. (1995) Mechanisms of toxicity and risk assessment. Toxicol. Lett. 77, 9-14.
21. Lotti M. (1996) The concept and target of promotion of axonopathies. Arch. Toxicol. S19, 331-336.
22. Lotti M., Caroldi S., Capodicasa E., and Moretto A. (1991) Promotion of organophosphate-induced delayed polyneuropathy by phenylmethanesulfonyl fluoride. Toxicol. Appl. Pharmacol. 108, 234-241.
23. Lush M., Read D., and Glynn P. (1996) Molecular cloning of neuropathy target esterase. Toxicol. Lett. 88 (Suppl. 1), 27.
24. Moretto A., Bertolazzi M., and Lotti M. (1994) The phosphorothioic acid O-(2-chloro-2,3,3-trifluorocyclobutyl) O-ethyl S-propyl ester exacerbates organophosphate polyneuropathy without inhibition of neuropathy target esterase. Toxicol. Appl. Pharmacol. 129, 133-137.
25. Richardson R. J., Davis C. S., and Johnson M. K. (1979) Subcellular distribution of marker enzymes and of neurotoxic esterase in adult hen brain. J. Neurochem. 32, 607-615.
26. 3H]diisopropyl phosphorofluoridate. J. Neurochem. 58, 135-141.
27. Sammons D. W., Adams L. D., and Nishizawa E. E. (1981) Ultra-sensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2, 135-141.
28. Tormo N., Gimeno J. R., Sogorb M. A., Díaz-Alejo N., and Vilanova E. (1993) Soluble and particulate organophosphorous neuropathy target esterase in brain and sciatic nerve of the hen, cat, rat, and chick. J. Neurochem. 61, 2164-2168.
29. Towbin H., Staehelin T., and Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
30. Vilanova E., Barril J., Carrera V., and Pellin M. C. (1990) Soluble and particulate forms of the organophosphorus neuropathy target esterase in hen sciatic nerve. J. Neurochem. 55, 1258-1265.
31. Williams D. G. and Johnson M. K. (1981) Gel electrophoretic identification of hen brain neurotoxic esterase, labelled with tritiated di-isopropyl phosphorofluoridate. Biochem. J. 199, 323-333.