The cloned rat hydrolytic enzyme responsible for the breakdown of anandamide also catalyzes its formation via the condensation of arachidonic acid and ethanolamine

Neuroscience Letters

Volumn 234, Issue 1, 1997, Pages 59-62

  Arreaza, Gladys ^a   Devane, William A ^b   Omeir, Romelda L ^a   Sajnani, Gina ^a   Kunz, Jeffrey ^a   Cravatt, Benjamin F ^c   Deutsch, Dale G ^a  

^a STONY BROOK UNIVERSITY   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Amidase; Amidohydrolase; Anandamide; Cannabinoids; Fatty acid amide hydrolase; Synthase

Indexed keywords

ANANDAMIDE; ARACHIDONIC ACID; CANNABINOID RECEPTOR; ETHANOLAMINE;

ANIMAL CELL; ARTICLE; ENZYME ACTIVITY; MOLECULAR CLONING; NEUROTRANSMISSION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; RAT;

AMIDOHYDROLASES; ANIMALS; ARACHIDONIC ACID; BRAIN; CATALYSIS; CLONING, MOLECULAR; COS CELLS; ETHANOLAMINE; HYDROLYSIS; RATS; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0030866539     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(97)00673-3     Document Type: Article

References (18)

1. Burstein S.H., Hunter S.A. Stimulation of anandamide biosynthesis in N-18TG2 neuroblastoma cells by delta 9-tetrahydrocannabinol (THC). Biochem. Pharmacol. 49:1995;855-858.
2. Cravatt B.F., Giang D.K., Mayfield S.P., Boger D.L., Lerner R.A., Gilula N.B. Molecular characterization of an enzyme that degrades neuromodulatory fatty-acid amides. Nature. 384:1996;83-87.
3. Cravatt B.F., Prospero-Garcia O., Siuzdak G., Gilula N.B., Henriksen S.J., Boger D.L., Lerner R.A. Chemical characterization of a family of brain lipids that induce sleep. Science. 268:1995;1506-1509.
4. Desarnaud F., Cadas H., Piomelli D. Anandamide amidohydrolase activity in rat brain microsomes. Identification and partial characterization. J. Biol. Chem. 270:1995;6030-6035.
5. Deutsch D.G., Chin S.A. Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist. Biochem. Pharmacol. 46:1993;791-796.
6. Deutsch D.G., Lin S., Hill W.A.G., Morse K.L., Salehani D., Arreaza G., Omeir R.L., Makriyannis A. Fatty acid sulfonyl fluorides inhibit anandamide metabolism and bind to the cannabinoid receptor. Biochem. Biophys. Res. Commun. 231:1997;217-221.
7. Deutsch D.G., Omeir R., Arreaza G., Salehani D., Prestwich G.D., Huang Z., Howlett A. Methyl arachidonyl fluorophosphonate: a potent irreversible inhibitor of anandamide amidase. Biochem. Pharmacol. 53:1997;255-260.
8. Devane W.A., Axelrod J. Enzymatic synthesis of anandamide, an endogenous ligand for the cannabinoid receptor, by brain membranes. Proc. Natl. Acad. Sci. USA. 91:1994;6698-6701.
9. Devane W.A., Hanus L., Breuer A., Pertwee R.G., Stevenson L.A., Griffin G., Gibson D., Mandelbaum A., Etinger A., Mechoulam R. Isolation and structure of a brain constituent that binds to the cannabinoid receptor. Science. 258:1992;1946-1949.
10. Di Marzo V., Fontana A., Cadas H., Schinelli S., Cimino G., Schwartz J.C., Piomelli D. Formation and inactivation of endogenous cannabinoid anandamide in central neurons. Nature. 372:1994;686-691.
11. Hillard C.J., Wilkison D.M., Edgemond W.S., Campbell W.B. Characterization of the kinetics and distribution of N-arachidonylethanolamine (anandamide) hydrolysis by rat brain. Biochim. Biophys. Acta. 1257:1995;249-256.
12. Kruszka K.K., Gross R.W. The ATP- and CoA-independent synthesis of arachidonoylethanolamide. A novel mechanism underlying the synthesis of the endogenous ligand of the cannabinoid receptor. J. Biol. Chem. 269:1994;14345-14348.
13. Mechoulam R., Benshabat S., Hanus L., Fride E., Vogel Z., Bayewitch M., Sulcova A.E. Endogenous cannabinoid ligands - chemical and biological studies. J. Lipid Mediat. Cell Signal. 14:1996;45-49.
14. 14C] as a substrate for anandamide amidase. Life Sci. 56:1995;1999-2005.
15. Paria B.C., Deutsch D.D., Dey S.K. The uterus is a potential site for anandamide synthesis and hydrolysis - differential profiles of anandamide synthase and hydrolase activities in the mouse uterus during the periimplantation period. Mol. Reprod. Dev. 45:1996;183-192.
16. Schmid H.H.O., Schmid P.C., Natarajan V. N-Acylated glycerophospholipids and their derivatives. Prog. Lipid Res. 29:1990;1-43.
17. Sugiura T., Kondo S., Sukagawa A., Tonegawa T., Nakane S., Yamashita A., Ishima Y., Waku K. Transacylase-mediated and phosphodiesterase-mediated synthesis of N-arachidonoylethanolamine, an endogenous cannabinoid-receptor ligand, in rat brain microsomes - comparison with synthesis from free arachidonic acid and ethanolamine. Eur. J. Biochem. 240:1996;53-62.
18. Ueda N., Kurahashi Y., Yamamoto S., Tokunaga T. Partial purification and characterization of the porcine brain enzyme hydrolyzing and synthesizing anandamide. J. Biol. Chem. 270:1995;23823-23827.