Replication of in vitro tobravirus recombinants shows that the specificity of template recognition is determined by 5' non-coding but not 3' non-coding sequences

Journal of General Virology

Volumn 78, Issue 8, 1997, Pages 2085-2088

  Mueller, Anna Maria ^a   Mooney, Adele L ^a   MacFarlane, Stuart A ^a  

^a SCOTTISH CROP RESEARCH INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COAT PROTEIN; COMPLEMENTARY DNA; RNA DIRECTED RNA POLYMERASE; VIRUS RNA;

ARTICLE; DNA TEMPLATE; NONHUMAN; PEA; PLANT VIRUS; PRIORITY JOURNAL; PROMOTER REGION; TOBACCO; VIRUS RECOMBINANT; VIRUS REPLICATION;

NICOTIANA TABACUM; PEA EARLY BROWNING VIRUS; PISUM SATIVUM; TOBACCO RATTLE VIRUS; TOBRAVIRUS;

EID: 0030849403     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-78-8-2085     Document Type: Article

References (19)

1. Angenent, G. C., Linthorst, H. J. M., van Belkum, A. F., Cornelissen, B. J. C. & Bol, J. F. (1986). RNA2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14, 4673-4682.
2. Cornelissen, B. J. C., Linthorst, H. J. M., Brederode, F. T. & Bol, J. F. (1986). Analysis of the genome structure of tobacco rattle virus strain PSG. Nucleic Acids Research 14, 2157-2169.
3. Goulden, M. G., Lomonossoff, G. P., Davies, J. W. & Wood, K. R. (1990). The complete nucleotide sequence of PEBV RNA2 reveals the presence of a novel open reading frame and provides insights into the structure of tobraviral subgenomic promoters. Nucleic Acids Research 18, 4507-4512.
4. Harrison, B. D. & Robinson, D. J. (1986). Tobraviruses. In The Plant Viruses, vol. 2, pp. 339-369. Edited by M. H. V. Van Regenmortel & H. Fraenkel-Conrat. New York: Plenum Press.
5. Hernandez, C., Mathis, A., Brown, D. J. F. & Bol, J. F. (1995). Sequence of RNA2 of a nematode-transmissible isolate of tobacco rattle virus. Journal of General Virology 76, 2847-2851.
6. Hernandez, C., Carette, J. E., Brown, D. J. F. & Bol, J. F. (1996). Serial passage of tobacco rattle virus under different selection conditions results in deletion of structural and nonstructural genes in RNA2. Journal of Virology 70, 4933-4940.
7. MacFarlane, S. A. & Brown, D. J. F. (1995). Sequence comparison of RNA2 of nematode-transmissible and nematode non-transmissible isolates of pea early browning virus suggests that the gene encoding the 29 kDa protein may be involved in nematode transmission. Journal of General Virology 76, 1299-1304.
8. MacFarlane, S. A., Wallis, C. V., Taylor, S. C., Goulden, M. G., Wood, K. R. & Davies, J. W. (1991). Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early browning virus. Virology 182, 124-129.
9. MacFarlane, S. A., Wallis, C. V. & Brown, D. J. F. (1996). Multiple virus genes involved in the nematode transmission of pea early browning virus. Virology 219, 417-422.
10. Michael, S. F. (1994). Mutagenesis by incorporation of a phosphorylated oligo during PCR amplification. BioTechniques 16, 410-412.
11. Ploeg, A. T., Brown, D. J. F. & Robinson, D. J. (1992). The association between species of Trichodorus and Paratrichodorus vector nematodes and serotypes of tobacco rattle tobravirus. Annals of Applied Biology 121, 619-630.
12. Roberts, I. M. (1986). Immunoelectron microscopy of extracts of virus-infected plants. In Electron Microscopy of Proteins, vol. 5, Virus Structure, pp. 293-357. Edited by J. R. Harris & R. W. Home. New York: Academic Press.
13. Robinson, D. J. (1994). Sequences at the ends of RNA-2 of 16, a recombinant tobravirus. Archives of Virology 9, 245-251.
14. Robinson, D. J. & Harrison, B. D. (1985). Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses. Journal of General Virology 66, 171-176.
15. Robinson, D. J., Mayo, M. A., Fritsch, C., Jones, A. T. & Raschke, J. H. (1983). Origin and messenger activity of two small RNA species found in particles of tobacco rattle virus strain SYM. Journal of General Virology 64, 1591-1599.
16. Robinson, D. J., Hamilton, W. D. O., Harrison, B. D. & Baulcombe, D. C. (1987). Two anomalous tobravirus isolates: evidence for RNA recombination in nature. Journal of General Virology 68, 2551-2561.
17. Steinberg, R. A. & German, K. B. (1994). A high-yield method for site-directed mutagenesis using polymerase chain reaction and three primers. Analytical Biochemistry 219, 155-157).
18. Verwoerd, T. C., Dekker, B. M. M. & Hoekema, A. (1989). A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17, 2362.
19. Wallis, C. V. (1992). The molecular biology of pea early browning virus RNA2. PhD thesis, University of Birmingham, UK.