A direct method for the chromosomal assignment of DNA markers in Leishmania

Gene

Volumn 194, Issue 1, 1997, Pages 77-80

  Wincker, Patrick ^a   Ravel, Christophe ^a   Britto, Constança ^a   Dubessay, Pascal ^a   Bastien, Patrick ^a   Pagès, Michel ^a   Blaineau, Christine ^a  

^a CNRS   (France)

Author keywords

Leishmania genome map; Linkage groups; Parasite genome; Pulsed field gel electrophoresis

Indexed keywords

DNA MARKER;

ARTICLE; CHROMOSOME SIZE; GENE MAPPING; KARYOTYPE; LEISHMANIA; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS;

ANIMALS; CHROMOSOME BANDING; CHROMOSOME MAPPING; DNA, PROTOZOAN; ELECTROPHORESIS, GEL, PULSED-FIELD; GENES, PROTOZOAN; GENETIC MARKERS; GENOME; KARYOTYPING; LEISHMANIA; POLYMORPHISM, GENETIC;

PROTOZOA;

EID: 0030846355     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00162-5     Document Type: Article

References (10)

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2. Iovannisci, D.M., Beverley, S. M., 1989. Structural alterations of chromosome 2 in Leishmania major as evidence for diploidy, including spontaneous amplification of the mini-exon array. Mol. Biochem. Parasitol. 34, 177-188.
3. Levick, M.P., Blackwell, J.M., Connor, V., Coulson, R.M.R., Miles, A., Smith, H.E., Wan, K.L., Ajioka, J.W., 1996. An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes. Mol. Biochem. Parasitol. 76, 345-348.
4. Pagès, M., Bastien, P., Veas, F., Rossi, V., Bellis, M., Wincker, P., Rioux, J.A., Roizès, G., 1989. Chromosome size and number polymorphims in Leishmania infantum suggest amplification/deletion and possible genetic exchange. Mol. Biochem. Parasitol. 36, 161-168.
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6. Ravel, C., Wincker, P., Blaineau, C., Britto, C., Bastien, P., Pagès, M., 1996. Medium-range restriction maps of five chromosomes of Leishmania infantum and localization of size-variable regions. Genomics 35, 509-516.
7. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Scholler, J.K., Reed, S.G., Stuart, K., 1986. Molecular karyotype of species and sub-species of Leishmania. Mol. Biochem. Parasitol. 20, 279-283.
9. Shapira, M., Pinelli, E., 1989. Heat-shock protein hsp83 of Leishmania mexicana amazonensis is an abundant cytoplasmic protein with a tandemly repeated genomic arrangement. Eur. J. Biochem. 185, 231-236.
10. Wincker, P., Ravel, C., Blaineau, C., Pagès, M., Jauffret, Y., Dedet, J.P., Bastien, P., 1996. The Leishmania genome comprises 36 chromosomes conserved across widely divergent human pathogenic species. Nucleic Acids Res. 24, 1688-1694.