An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes

Molecular and Biochemical Parasitology

Volumn 76, Issue 1-2, 1996, Pages 345-348

  Levick, Mark P ^a   Blackwell, Jenefer M ^a   Connor, Vivienne ^a   Coulson, Richard M R ^a   Miles, Alistair ^a   Smith, Heather E ^a   Wan, Kiew Lian ^a   Ajioka, James W ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

cDNA; expressed sequence tag; Leishmania major; promastigote

Indexed keywords

COMPLEMENTARY DNA;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; LEISHMANIA MAJOR; NONHUMAN; PRIORITY JOURNAL; PROMASTIGOTE; SEQUENCE TAGGED SITE;

ANIMALIA; LEISHMANIA MAJOR;

EID: 0029989495     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-6851(95)02569-3     Document Type: Article

References (15)

1. Chakrabarti, D., Reddy, G.R., Dame, J.B., Almira, B.C., Laipis, P.J., Ferl, R.J., Yang, T.P., Rowe, T.C. and Schuster, S.M. (1994) Analysis of expressed sequence tags from Plasmodium falciparum. Mol. Biochem. Parasitol. 66, 97-104.
2. El Sayed, N.M.A., Alarcon, C.M., Beck, J.C., Sheffield, V.C. and Donelson, J.E. (1995) cDNA expressed sequence tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite. Mol. Biochem. Parasitol. 73, 75-90.
3. Wan, K.L., Blackwell, J.M. and Ajioka, J.W. (In Press) Toxoplasma gondii tachyzoite expressed sequence tags (ESTs):insight into life-cycle stage specific gene expression. Mol. Biochem. Parasitol.
4. Perry, K.L., Watkins, K.P. and Agabian, N. (1987) Trypanosome mRNAs have unusual 'cap 4' structures acquired by addition of a spliced leader. Proc. Natl. Acad. Sci. USA 84, 8190-8194.
5. Conrad, R., Thomas, T. and Blumenthal, T. (1991) Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a transspliced gene. Mol. Cell Biol. 11, 1921-1926.
6. Martin, S.A.M., Thompson, F.J, and Devaney, E. (1995) The construction of spliced-leader cDNA libraries from the filarial nematode Brugia pahangi. Mol. Biochem. Parasitol. 70, 241-245.
7. Chomezynski, P. and Saachi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
8. Ajioka, J.W., Melvile, S.E., Coulson, R.M.R., Espinoza, J.R., Khan, S.M. and Wan, K.-L. (1995) Techniques associated with protozoan parasite genome analysis. In: Methods in Molecular Genetics (Adolph, K.W., ed.), Vol. 6, pp. 30-47, Academic Press, London.
9. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
10. McCombie, W.R., Adams, M.D., Kelley, J.M., Fitzgerld, M.G., Utterback, T.R., Khan, M., Dubnick, K., Kerlavage, A.R., Venter, J.C. and Fields, C. (1992) Caenorhabditis elegans expressed sequence tags identify gene families and potential disease homologs. Nature Genet. 1, 124-131.
11. Franco, G.R., Adams, M.D., Scares, M.B., Simpson, A.J.G., Venter, J.C. and Pena, S.D.J. (1995) Identification of new Schistosoma mansoni genes by the EST strategy using a directional cDNA library. Gene 152, 141-147.
12. Richter, D., Reynolds, S.R. and Harn, D.A. (1993) Candidate vaccine antigens that stimulate the cellular immune-response of mice vaccinated with irradiated cercariae of Schistosoma mansoni. J. Immunol. 151, 256-265.
13. Lawrence, F. and Robertgero, M. (1993) Distribution of macromolecular methylations in promastigotes of Leishmania donovani and impact of sinefungin. J. Eukaryot. Microbiol. 40, 581-589.
14. Jambou, R., Hatin, I. and Jaureguiberry, G. (1995) Evidence by in-situ hybridisation for stage-specific expression of the ADP/ATP translocator messenger-RNA in Plasmodium falciparum Exp. Parasitol. 80, 568-571.
15. Flinn, H.M., Rangarajan, D. and Smith, D.F. (1994) Expression of a hydrophilic surface protein in infective stages of Leishmania major. Mol. Biochem. Parasitol. 65, 259-270.