Search for cytomegalovirus-specific immunoglobulin M: Comparison between a new western blot, conventional western blot, and nine commercially available assays

Clinical and Diagnostic Laboratory Immunology

Volumn 4, Issue 4, 1997, Pages 483-486

  Lazzarotto, T ^a   Brojanac, S ^b   Maine, G T ^b   Landini, M P ^a,c  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b ABBOTT LABORATORIES   (United States)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M;

ARTICLE; DIAGNOSTIC ACCURACY; FEMALE; HUMAN; HUMAN CYTOMEGALOVIRUS; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; VIRUS CHARACTERIZATION; VIRUS DETECTION;

EID: 0030844786     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.4.4.483-486.1997     Document Type: Article

References (20)

1. Basson, J., J. C. Tardy, and M. Aymard. 1989. Pattern of anti-cytomegalovirus IgM antibodies determined by immunoblotting. A study of kidney graft recipients developing a primary or recurrent CMV infection. Arch. Virol. 108:259-270.
2. Chee, M. S., A. T. Bankier, S. Beck, R. Bohni, C. M. Brown, T. Cerny, T. Horsnell, C. A. Hutchinson, T. Kouzarides, and J. A. Martinetti. 1990. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD 169. Curr. Top. Microbiol. Immunol. 154:125-169.
3. Gold, D., R. Ashley, H. H. Handsfield, M. Verdon, L. Leach, J. Mills, L. Drew, and L. Corey. 1988. Immunoblot analysis of the humoral immune response in primary cytomegalovirus infection. J. Infect. Dis. 157:319-325.
4. Landini, M. P., and A. Ripalti. 1982. A DNA-nicking activity associated with the nucleocapsid of human cytomegalovirus. Arch. Virol. 78:351-356.
5. Landini, M. P., E. Rossier, and H. Schmitz. 1988. Antibodies to human cytomegalovirus structural polypeptides during primary infection. J. Virol. Methods 22:309-317.
6. Landini, M. P., G. Mirolo, B. Baldassari, and M. La Placa. 1985. Antibody response to cytomegalovirus structural polypeptides studied by immunoblotting. J. Med. Virol. 17:303-311.
7. Landini, M. P., T. Lazzarotto, G. T. Maine, A. Ripalti, and R. Flanders. 1995. Recombinant mono- and polyantigens to detect cytomegalovirus-specific immunoglobulin M in human sera by enzyme immunoassay. J. Clin. Microbiol. 33:2535-2542.
8. Lazzarotto, T., B. Dalla Casa, B. Campisi, and M. P. Landini. 1992. Enzyme-linked immunoadsorbent assay for the detection of cytomegalovirus-IgM: comparison between eight commercial kits, immunofluorescence and immunoblotting. J. Clin. Lab. Anal. 6:216-218.
9. Lazzarotto, T., G. T. Maine, P. Dal Monte, H. Frush, K. Shi, and M. P. Landini. 1996. Detection of serum immunoglobulin M to human cytomeg-alovirus by Western blotting correlates better with virological data than detection by conventional enzyme immunoassay. Clin. Diagn. Lab. Immunol. 3:597-600.
10. Lazzarotto, T., G. T. Maine, P. Dal Monte, A. Ripalti, and M. P. Landini. 1997. A novel Western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection. J. Clin. Microbiol. 35:393-397.
11. Maine, G. T., T. Lazzarotto, L. E. Chovan, R. Flanders, and M. P. Landini. 1996. The DNA-binding protein pUL57 of human cytomegalovirus: comparison of specific immunoglobulin M (IgM) reactivity with IgM reactivity to other major target antigens. Clin. Diagn. Lab. Immunol. 3:358-360.
12. Nielsen, C. M., K. Hansen, H. M. K. Andersen, J. Gerstoft, and B. F. Vestergaard. 1987. An enzyme labelled nuclear antigen immunoassay for detection of cytomegalovirus IgM antibodies in human serum: specific and nonspecific reactions. J. Med. Virol. 22:67-76.
13. Ripalti, A., M. C. Boccuni, F. Campanini, G. Bergamini, T. Lazzarotto, M. C. Battista, B. Dalla Casa and M. P. Landini. 1994. Construction of a polyepitope fusion antigen of human cytomegalovirus ppUL32 and detection of specific antibodies by ELISA. Microbiologica 18:1-12.
14. Ripalti, A., P. Dal Monte, M. C. Boccuni, F. Campanini, G. Bergamini, T. Lazzarotto, B. Campisi, Q. Ruan, and M. P. Landini. 1994. Prokaryotic expression of a large fragment of the most antigenic cytomegalovirus DNA-binding proteins (ppUL44) and its reactivity with human antibodies. J. Virol. Methods 46:39-50.
15. Robinson, J. M., T. J. Pilot-Matias, S. D. Pratt, C. B. Patel, T. S. Bevirt, and J. C. Hunt. 1993. Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis. J. Clin. Microbiol. 31:629-635.
16. Stagno, S., M. K. Tinker, C. Elrod, D. Fucillo, G. Cloud, and A. J. O'Beirne. 1985. Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants. J. Clin. Microbiol. 21:930-935.
17. van der Biji, W., R. Torensma, W. J. Son, J. Schirm, A. M. Tegzess, and T. H. The. 1988. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. J. Med. Virol. 25:179-188.
18. van Loon, N. M., F. W. A. Hessen, J. T. M. van der Logt, and J. van der Veen. 1981. Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus. J. Clin. Microbiol. 13:416-422.
19. Vornhagen, R., B. Plachter, W. Hinderer, T. H. The, J. Van Zanten, L. Matter, C. A. Schmidt, H.-H. Sonneborg, L. Matter, and G. Jahn. 1994. Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens. J. Clin. Microbiol. 32:981-986.
20. Vornhagen, R., W. Hinderer, H.-H. Sonneborg, G. Bein, L. Matter, T. H. The, G. Jahn, and B. Plachter. 1995. The DNA-binding protein pUL57 of human cytomegalovirus is a major target antigen for the immunoglobulin M antibody response during acute infection. J. Clin. Microbiol. 33:1927-1930.