A novel western blot test containing both viral and recombinant proteins for anticytomegalovirus immunoglobulin M detection

Journal of Clinical Microbiology

Volumn 35, Issue 2, 1997, Pages 393-397

  Lazzarotto, T ^a   Maine, G T ^b   Dal Monte, P ^a   Ripalti, A ^a   Landini, M P ^a,c  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b ABBOTT LABORATORIES   (United States)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN M; RECOMBINANT PROTEIN; VIRUS PROTEIN;

ARTICLE; BLOOD DONOR; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC TEST; HUMAN; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; NONHUMAN; ORGAN TRANSPLANTATION; PREGNANCY; PRIORITY JOURNAL; VIRUS INFECTION;

EID: 0031036769     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.2.393-397.1997     Document Type: Article

References (28)

1. Basson, J., J. C. Tardy, and M. Aymard. 1989. Pattern of anti-cytomegalovirus IgM antibodies determined by immunoblotting. A study of kidney graft recipients developing a primary or recurrent CMV infection. Arch. Virol. 108:259-270.
2. Boland, G. J., G. C. de Gast, R. J. Hené, G. Jambroes, R. Donckerwolcke, T. H. The, and G. C. Mudde. 1990. Early detection of active cytomegalovirus (CMV) infection after heart and kidney transplantation by testing for immediate early antigenemia and influence of cellular immunity on the occurrence of CMV infection. J. Clin. Microbiol. 28:2069-2073.
3. Chee, M. S., A. T. Bankier, S. Beck, R. Bohni, C. M. Brown, T. Cerny, T. Horsnell, C. A. Hutchinson, T. Kouzarides, and J. A. Martinetti. 1990. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD 169. Curr. Top. Microbiol. Immunol. 154:125-169.
4. Dolan, J., J. D. Briggs, and G. B. Clements. 1989. Antibodies to cytomegalovirus in renal allograft recipients: correlation with isolation of virus. J. Clin. Pathol. 42:1070-1077.
5. Gleaves, C. A., T. F. Smith, E. A. Shuster, and G. R. Pearson. 1984. Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by usine low-speed centrifugation and monoclonal antibody to an early antigen. J. Clin. Microbiol. 19:917-919.
6. Gold, D., R. Ashley, H. H. Handsfield, M. Verdon, L. Leach, J. Mills, L. Drew, and L. Corey. 1988. Immunoblot analysis of the humoral immune response in primary cytomegalovirus infection. J. Infect. Dis. 157:319-325.
7. Jiwa, N. M., G. W. Gemert, A. K. Raap, F. M. Van der Riijjke, A. Mulder, P. F. Lens, M. M. M. Salimans, F. E. Zwaan, W. Van Dorp, and M. Van der Ploeg. 1989. Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 48:72-76.
8. Kraat, Y. J., F. S. Stals, M. H. L. Christiaans, T. Lazzarotto, M. P. Landini, and C. A. Bruggeman. 1996. IgM antibody detection for 38 (ppUL80a) and 150 (ppUL32) kDa proteins by immunoblotting: the earliest parameter for acute cytomegalovirus infection in renal transplant recipients. J. Med. Virol. 48:289-294
9. Landini, M. P., and A. Ripalti. 1982. A DNA-nicking activity associated with the nucleocapsid of human cytomegalovirus. Arch. Virol. 78:351.
10. Landini, M. P., E. Rossier, and H. Schmitz. 1988. Antibodies to human cytomegalovirus structural polypeptides during primary infection. J. Virol. Methods 22:309-317.
11. Landini, M. P., G. Mirolo, B. Baldassari, and M. La Placa. 1985. Antibody response to cytomegalovirus structural polypeptides studied by immunoblotting. J. Med. Virol. 17:303-311.
12. Landini, M. P., T. Lazzarotto, G. T. Maine, A. Ripalti, and R. Flanders. 1995. Recombinant mono- and polyantigens to detect cytomegalovirus-specific immunoglobulin M in human sera by enzyme immunoassay. J. Clin. Microbiol. 33:2535-2542.
13. Lazzarotto, T., B. Dalla Casa, B. Campisi, and M. P. Landini. 1992. Enzyme-linked immunoadsorbent assay for the detection of cytomegalovirus-IgM: comparison between eight commercial kits, immunofluorescence and immunoblotting. J. Clin. Lab. Anal. 6:216-218.
14. Lazzarotto, T., G. Furlini, M. C. Re, E. Ramazzotti, B. Campisi, and M. P. Landini. 1994. Human cytomegalovirus replication correlates with differentiation in a hematopoietic progenitor cell line and can be modulated by HIV. Arch. Virol. 135:13-28.
15. Lazzarotto, T., G. T. Maine, P. Dal Monte, H. Frush, K. Shi, and M. P. Landini. 1996. Detection of serum immunoglobulin M to human cytomegalovirus by Western blotting correlates better with virological data than detection by conventional enzyme immunoassay. Clin. Diagn. Lab. Immunol. 3:597-600.
16. Maine, G. T., T. Lazzarotto, L. E. Chovan, R. Flanders, and M. P. Landini. 1996. The DNA-binding protein pUL57 of human cytomegalovirus: comparison of specific immunoglobulin M (IgM) reactivity with IgM reactivity to other major target antigens. Clin. Diagn. Lab. Immunol. 3:338-360.
17. Marsano, L., R. P. Perrillo, M. W. Flye, D. W. Hanto, E. D. Spitzer, J. R. Thomas, P. R. Murray, D. W. Windus, E. M. Brunt, and G. A. Storch. 1990. Comparison of culture and serology for the diagnosis of cytomegalovirus infection in kidney and liver transplant recipients. J. Infect. Dis. 161:454-461.
18. Nielsen, C. M., K. Hansen, H. M. K. Andersen, J. Gerstoft, and B. F. Vestergaard. 1987. An enzyme labelled nuclear antigen immunoassay for detection of cytomegalovirus IgM antibodies in human serum: specific and non specific reactions. J. Med. Virol. 22:67-76.
19. Nielsen, S. L., I. Sørensen, and H. K. Andersen. 1988. Kinetics of specific immunoglobulins M, E, A, and G in congenital, primary, and secondary cytomegalovirus infection studied by antibody-capture enzyme-linked immunosorbent assay. J. Clin. Microbiol. 26:654-661.
20. Revello, M. G., M. Zavattoni, E. Percivalle, P. Grossi, and G. Gerna. 1989. Correlation between immunofluorescent detection of human cytomegalovirus immediate early antigens in polymorphonuclear leukocytes and viremia. J. Infect. Dis. 160:159-160.
21. Ripalti, A., M. C. Boccuni, F. Campanini, G. Bergamini, T. Lazzarotto, M. C. Battista, B. Dalla Casa, and M. P. Landini. 1994. Construction of a polyepitope fusion antigen of human cytomegalovirus ppUL32 and detection of specific antibodies by ELISA. Microbiologica 18:1-12.
22. Ripalti, A., P. Dal Monte, M. C. Boccuni, F. Campanini, G. Bergamini, T. Lazzarotto, B. Campisi, Q. Ruan, and M. P. Landini. 1994. Prokaryotic expression of a large fragment of the most antigenic cytomegalovirus DNA-binding proteins (ppUL44) and its reactivity with human antibodies. J. Virol. Methods 46:39-511.
23. Robinson, J. M., T. J. Pilot-Matias, S. D. Pratt, C. B. Patel, T. S. Bevirt, and J. C. Hunt. 1993. Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis. J. Clin. Microbiol. 31:629-635.
24. Stagno, S., M. K. Tinker, C. Elrod, D. Fucillo, G. Cloud, and A. J. O'Beirne. 1985. Immunoglobulin M antibodies detected by enzyme-linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infections in pregnant women and newborn infants. J. Clin. Microbiol. 21:930-935.
25. van der Biji, W., R. Torensma, W. J. Son, J. Schirm, A. M. Tegzess, and T. H. The. 1988. Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leukocytes. J. Med. Virol. 25:179-188.
26. van Loon, N. M., F. W. A. Hessen, J. T. M. van der Logt, and J. van der Veen. 1981. Direct enzyme-linked immunosorbent assay that uses peroxidase-labeled antigen for determination of immunoglobulin M antibody to cytomegalovirus. J. Clin. Microbiol. 13:416-422.
27. Vornhagen, R., B. Plachter, W. Hinderer, T. H. The, J. Van Zanten, L. Matter, C. A. Schmidt, H.-H. Sonneborg, L. Matter, and G. Jahn. 1994. Early serodiagnosis of acute human cytomegalovirus infection by enzyme-linked immunosorbent assay using recombinant antigens. J. Clin. Microbiol. 32:981-986.
28. Vornhagen, R., W. Hinderer, H.-H. Sonneborg, G. Bein, L. Matter, T. H. The, G. Jahn, and B. Plachter. 1995. The DNA-binding protein pUL57 of human cytomegalovirus is a major target antigen for the immunoglobulin M antibody response during acute infection. J. Clin. Microbiol. 33:1927-1930.