Interaction of N-tosyl-L-phenylalanylchloromethane with Thermus thermophilus elongation factor Tu

European Journal of Biochemistry

Volumn 247, Issue 1, 1997, Pages 59-65

  Nawrot, Barbara ^a   Hillenbrand, Rainer ^a   Limmer, Stefan ^a   Grillenbeck, Norbert ^a   Sprinzl, Mathias ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Affinity labeling; Aminoacyl tRNA; Elongation factor Tu; GTPase; Translation

Indexed keywords

ELONGATION FACTOR TU; TOSYLPHENYLALANYL CHLOROMETHYL KETONE;

ARTICLE; BINDING SITE; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN SYNTHESIS; THERMUS THERMOPHILUS;

THERMUS THERMOPHILUS;

EID: 0030839061     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.t01-1-00059.x     Document Type: Article

References (31)

1. Abel, K., Yoder, M. D., Hilgenfeld, R. & Jurnak, F. (1996) An a to b conformational switch in EF-Tu, Structure (Loud.) 4, 1153-1159.
2. Ahmadian, M. R., Kreutzer, R. & Sprinzl, M. (1991) Overproduction of the Thermus thermophilus elongation factor Tu in Escherichia coli, Biochimie (Paris) 73, 1037-1043.
3. Anborgh, P. H., Parmeggiani, A. & Jonák, J. (1992) Site-directed mutagenesis of elongation factor Tu. Eur. J. Biochem. 208, 251-257.
4. Berchtold, H., Reshetnikova, L., Reiser, C. O. A., Schirmer, N. K., Sprinzl, M. & Hilgenfeld, R. (1993) Crystal structure of active elongation factor Tu reveals major domain rearrangements, Nature 365, 126-132.
5. Blank, J., Nock, S., Kreutzer, R. & Sprinzl, M. (1996) Elongation factor Ts from Thermus thermophilus. Overproduction in Escherichia coli, quaternary structure and interaction with elongation factor Tu, Eur. J. Biochem. 236, 222-227.
6. 4N) by Escherichia coli aminoacyl-tRNA synthetases, Proc. Natl Acad. Sci. USA 86, 8275-8279.
7. Brosius, J. & Holy, A. (1984) Regulation of ribosomal RNA promoters with a synthetic lac operator. Proc. Natl Acad. Sci. USA 81, 6929-6933.
8. Dutka, S., Meinnel, T., Lazennec, C., Mechulam, Y. & Blanquet, S. (1993) Role of the 1-72 base pair in tRNAs for the activity of Escherichia coli peptidyl-tRNA hydrolase, Nucleic Acids Res. 21, 4025-4030.
9. Phc, Biochimie (Paris) 75, 1159-1166.
10. Förster, C., Limmer, S., Zeidler, W. & Sprinzl, M. (1994) Effector region of the translation elongation factor EF-Tu · GTP complex stabilizes an orthoester acid intermediate structure of aminoacyl-tRNA in a ternary complex, Proc. Natl Acad. Sci. USA 91, 4254-4257.
11. Hanahan, D. (1983) Studies on transformation of Escherichia coll with plasmids, J. Mol. Biol. 166, 557-580.
12. Hiratsuka, T, (1983) New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes. Biochim. Biophys. Acta 742, 496-508.
13. Holmes, W. M., Hurd, R. E., Reid, B. R., Rimerman, R. A. & Hatfield, G. W. (1975) Separation of transfer ribonucleic acid by sepharose chromatography using reverse salt gradients. Proc. Natl Acad. Sci. USA 72, 1068-1071.
14. u from Escherichia coli, FEBS Lett. 98, 329-332.
15. Jonák, J., Petersen, T. E., Clark, B. F. C. & Rychlík, I. (1982) N-tosylL-phenylalanylchloromethane reacts with cysteine 81 in the molecule of elongation factor Tufrom Escherichia coli, FEBS Lett. 150, 485-488.
16. Jonák, J., Pokorná, K., Meloun, B. & Karas, K. (1986) Structural homology between elongation factors EF-Tu from Bacillus stearothermophilus and Escherichia coli in the binding site for aminoacyltRNA, Eur. J. Biochem. 154, 355-362.
17. Kjeldgaard, M., Nissen, P., Thirup, S. & Nyborg, J. (1943) The crystal structure of elongation factor EF-Tu from Thermus aquaticus in the GTP conformation. Structure (Lond.) 1, 35-50.
18. Limmer, S., Reiser, C. O. A., Schirmer, N. K., Grillenbeck, N. W. & Sprinzl, M. (1992) Nucleotide binding and GTP hydrolysis hy elongation factor Tu from Thermus thermophilus as monitored by proton NMR, Biochemistry 31, 2970-2977.
19. 13C NMR of enzymes, in Progress in nuclear magnetic resonance spectroscopy, vol. 17 (Emsley, J. W., Feeney, J. & Sutcliffe, L. M., eds) pp. 1-59, Pergamon Press, New York.
20. Phe, EF-Tu, and a GTP analog. Science 270, 1464-1472.
21. Ott, G., Faulhammer, H. G. & Sprinzl, M. (1989) Interaction of elongation factor Tu from Escherichia coli with aminoacyl-tRNA carrying a fluorescent reporter group on the 3′ terminus, Eur. J. Biochem. 184, 345-352.
22. Ott, G., Jonák, J., Abrahams, I. P. & Sprinzl, M. (1990) The influence of different modifications of elongation factor Tu from Escherichia coli on ternary complex formation investigated by fluorescence spectroscopy. Nucleic Acids Res. 18, 437-441.
23. Peter, M. E., Brockmöller, J., Jonák, J. & Sprinzl, M. (1989) Identification of the N-tosyl-L-phenylalanyl chloromethylketone modification site in Thermus thermophilus elongation factor Tu, FEBS Lett. 257, 219-222.
24. Polekhina, G., Thirup, S., Kjeldgaard, M., Nissen, P., Lippmann, C. & Nyborg, J. (1996) Helix unwinding in the effector region of elongation factor EF-Tu · GDP. Structure (Lond.) 4, 1141-1151.
25. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press. New York.
26. Schoellmann, G. & Shaw, E. (1963) Direct evidence for the presence of histidine in the active center of chymotrypsin. Biochemistry 2, 252-255.
27. Sprinzl, M., Kucharzewski, M., Hobbs, J. B. & Cramer, F. (1977) Specificity of elongation factor Tu from Escherichia coli with respect to attachment of the amino acid to the 2′ or 3′-hydroxyl group of the terminal adenosine of tRNA, Eur. J. Biochem. 78, 55-61.
28. Sprinzl, M. (1994) Elongation factor Tu: a regulatory GTPase with an integrated effector. Trends Biochem. Sci. 19, 245-250.
29. Sprinzl, M., Steegborn, C., Hübel, F. & Steinberg, S. (1996) Compilation of tRNA sequences and sequences of tRNA genes. Nucleic Acids Res. 24, 68-72.
30. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13-mp18 and pUC19 vectors. Gene (Amst.) 33, 103-119.
31. Zubay, G. (1966) Isolation of transfer RNA. in Procedures in nucleic acid research (Cantoni, G. L. & Davies, D. R., eds) pp. 455-460. Harper & Row, New York.