The multidrug-resistance P-glycoprotein (Pgp, MDR1) is an early marker of blood-brain barrier development in the microvessels of the developing human brain

Histochemistry and Cell Biology

Volumn 108, Issue 2, 1997, Pages 179-182

  Schumacher, Udo ^a   Mollgård, Kjeld ^b  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANTINEOPLASTIC AGENT; FORMALDEHYDE; GLYCOPROTEIN P; MONOCLONAL ANTIBODY; PROTON PUMP; WAX;

ARTICLE; BLOOD BRAIN BARRIER; BRAIN; BRAIN BLOOD VESSEL; BRAIN DEVELOPMENT; CONTROLLED STUDY; CROWN RUMP LENGTH; EMBRYO; FETUS; HUMAN; HUMAN TISSUE; IMMUNOREACTIVITY; MICROVASCULATURE; MULTIDRUG RESISTANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; VASCULAR ENDOTHELIUM;

ANTIBODIES, MONOCLONAL; BIOLOGICAL MARKERS; BLOOD-BRAIN BARRIER; BRAIN; GENES, MDR; HUMANS; IMMUNOHISTOCHEMISTRY; MICROCIRCULATION; P-GLYCOPROTEIN; PARAFFIN EMBEDDING;

EID: 0030838009     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180050159     Document Type: Article

References (16)

1. Breuer W, Slotki IN, Ausiello DA, Cabantchik IZ (1993) Induction of multidrug-resistance downregulates the expression of CFTR in colon epithelial cells. Am J Physiol 265:C1711-C1715
2. Chin KV, Ueda K, Pastan I, Gottesman MM (1992) Modulation of activity of the promoter of the human MDR1 gene by RAS and P53. Science 255:459-462
3. Cordon-Cardo C, O'Brien JP, Casals D, Rittman-Grauer L, Biedler JI, Melamed MR, Bertino JR (1989) Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites. Proc Natl Acad Sci USA 86:695-698
4. Cornwell MM, Smith DE (1993) A signal transduction pathway for activation of the mdr1 promoter involves the proto-oncogene c-raf kinase. J Biol Chem 268:15347-15350
5. Gottesman MM, Pastan I (1993) Biochemistry of the multidrug-resistance mediated by the multidrug transporter. Ann Rev Biochem 62:385-427
6. Juliano JR, Ling V (1976) A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochim Biophys Acta 455:152-162
7. Kioka N, Yamano Y, Komano T, Ueda K (1992) Heat-shock responsive elements in the induction of the multidrug-resistance gene (MDR1). FEBS Lett 301:37-40
8. Qin Y, Sato TN (1995) Mouse multidrug-resistance 1a/3 gene is the earliest known endothelial cell differentiation marker during blood-brain barrier development. Dev Dyn 202:172-180
9. Schinkel AH, Smit JJM, Tellingen O van, Beijnen JH, Wagenaar E, Deemter L van, Mol CAAM, Valk MA van der, Robanus-Maandag EC, Riele HPJ te, Berns AJM, Borst P (1994) Disruption of the mouse mdr1a P-glycoprotein gene leads to a deficiency in the blood-brain barrier and to increased sensitivity to drugs. Cell 77:491-502
10. Schinkel AH, Wagenaar E, Mol CAAM, Deemter L van (1996) P-glycoprotein in the blood-brain barrier of mice influences the brain penetration and pharmacological activity of many drugs. J Clin Invest 97:2517-2524
11. Schumacher U, Kretzschmar H, Pfüller U (1994) Staining of cerebral amyloid plaque glycoproteins in patients with Alzheimer's disease with the microglial specific lectin from mistletoe. Acta Neuropathol 87:422-424
12. Stewart PA, B, Iiveau R, Rogers KA (1996) Cellular localization of P-glycoprotein in brain versus gonadal capillaries. J Histochem Cytochem 44:679-685
13. Tanimura H, Kohno K, Sato S, Uchiumi T, Miyazaki M, Kobayashi M, Kuwano M (1992) The human multidrug-resistance 1 promoter has an element that responds to serum starvation. Biochem Biophys Res Commun 183:917-924
14. Thiebaut F, Tsuruo T, Hamada H, Gottesman MM, Pastan I, Willingham MC (1989) Immunohistochemical localization in normal tissues of different epitopes in the multidrug transport protein p 170 - evidence for localization in brain capillaries and crossreactivity of one antibody with a muscle protein. J Histochem Cytochem 37:159-164
15. Tóth K, Vaughan MM, Slocum HK, Arredonto MA, Takita H, Baker RM, Rustum YM (1994) New immunohistochemical "sandwich" staining method for mdr1 P-glycoprotein: detection with JSB- 1 monoclonal antibody in formalin-fixed, paraffin-embedded human tissues. Am J Pathol 144:227-236
16. Uchiumi T, Kohno K, Tanimura H, Hidaka K, Asakuno K, Abe H, Uchida Y, Kuwano M (1993) Involvement of protein kinase in environmental stress-induced activation of human multidrug-resistance 1 (MDR1) gene promoter. FEBS Lett 326:11-16