Activation of heat shock transcription factor 3 by c-Myb in the absence of cellular stress

Science

Volumn 277, Issue 5323, 1997, Pages 246-248

  Kanei Ishii, Chie ^a   Tanikawa, Jun ^a,d   Nakai, Akira ^b   Morimoto, Richard I ^c   Ishii, Shunsuke ^a  

^a RIKEN TSUKUBA INSTITUTE   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c NORTHWESTERN UNIVERSITY   (United States)

^d OSAKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HEAT SHOCK PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; CELL STRAIN 3T3; CONTROLLED STUDY; GENE INDUCTION; HEAT SHOCK; MOUSE; NONHUMAN; ONCOGENE C MYB; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN INDUCTION; TRANSCRIPTION INITIATION;

ANIMALS; CELL CYCLE; CELL LINE; CELL NUCLEUS; DNA; DNA-BINDING PROTEINS; PROMOTER REGIONS (GENETICS); PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-MYB; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSFECTION;

ANIMALIA; VERTEBRATA;

EID: 0030827157     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5323.246     Document Type: Article

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37. The preparation of GST-HSF fusion proteins, in vitro translation of c-Myb, and binding assays were as described (19) except for the binding buffer used [20 mM Hepes (pH 8.5), 1 mM EDTA, 5 mM dithiothreitol, and 0.1% NP-40], which contained 70 or 150 mM NaCl (for Fig. 3A).
38. We constructed the plasmid to express the Gal4-HSF fusion proteins containing the Gal4 DNA binding domain (amino acids 1 to 147) joined to either the DNA binding domain of HSF1 (amino acids 1 to 132), HSF2 (amino acids 1 to 130), or HSF3 (amino acids 1 to 125), by the polymerase chain reaction-based method with the use of the cytomegalovirus promoter-containing expression vector. The plasmid to express the Myb-VP16 fusion protein containing the DNA binding domain of c-Myb (amino acids 1 to 193) joined to the VP16 activation domain was made similarly with the use of the pcDNAS vector (Invitrogen). In the reporter plasmid, the thymidine kinase promoter containing three copies of the Gal4-binding site was linked to the luciferase gene. A mixture containing 4 p the luciferase reporter plasmid, 2 μg of the Gal4-HSF expression plasmid, 8 μg of either the VP16-Myb or VP16 expression plasmid, and 1 μg of pact-β-gal was transfected into NIH 3T3 cells, and luciferase assays were performed. Total plasmid DNA was adjusted to 17 μg by the addition of pact-1 DNA.
39. The 293T cells were transfected with either 0.3 μg of the chicken HSF3 expression plasmid or 8 μg of the mouse c-Myb expression plasmid, or a mixture of both plasmids. The transfected DNA also contained 0.5 μg of pact-β-gal DNA (10), and the total amount of plasmid DNA was adjusted to 10 μg by the addition of pact-1 DNA (10). Immunostaining and protein immunoblotting of HSF3 were performed as described (8).
40. We thank H. Akimaru for help in confocal laser microscopy and H. Nojima for encouragement. Supported in part by grants from the Ministry of Education, Science and Culture of Japan to S.I. C.K.-I. and J.T. contributed equally to this work.