The phosphatidylinositol 3-kinase inhibitor wortmannin markedly reduces chemotactic peptide-induced locomotion and increases in cytoskeletal actin in human neutrophils

European Journal of Pharmacology

Volumn 335, Issue 1, 1997, Pages 43-52

  Niggli, Verena ^a   Keller, Hansuli ^a  

^a UNIVERSITY OF BERN   (Switzerland)

Author keywords

Actinin; Actin; Cell locomotion; Neutrophils; Phosphatidylinositol 3 kinase; Wortmannin

Indexed keywords

ACTIN; CHEMOTACTIC PEPTIDE; CYTOSKELETON PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; WORTMANNIN;

ARTICLE; CELL MIGRATION; CONTROLLED STUDY; DOSE RESPONSE; HUMAN; HUMAN CELL; NEUTROPHIL; NORMAL HUMAN; PRIORITY JOURNAL;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ACTINS; ANDROSTADIENES; CHEMOTAXIS; CYTOSKELETAL PROTEINS; HUMANS; NEUTROPHILS; OLIGOPEPTIDES; PHOSPHATIDYLINOSITOL PHOSPHATES;

EID: 0030823258     PISSN: 00142999     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-2999(97)01169-2     Document Type: Article

References (48)

1. Amrein P.C., Stossel T.P. Prevention of degradation of human polymorphonuclear leukocyte proteins by diisopropylfluorophosphate. Blood. 56:1980;442-447.
2. Arcaro A., Wymann M.P.C. Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses. Biochem. J. 296:1993;297-301.
3. Baggiolini M., Dewald B., Schnyder J., Ruch W., Cooper P.H., Payne T.G. Inhibition of the phagocytosis-induced burst by the fungal metabolite wortmannin and some analogues. Expt. Cell Res. 169:1987;408-418.
4. 2+ transient for fMet-Leu-Phe induced actin polymerization in human neutrophils. Eur. J. Cell Biol. 42:1986;338-343.
5. Bokoch G.M. Chemoattractant signaling and leukocyte activation. Blood. 86:1995;1649-1660.
6. Böyum A. Separation of leukocytes from blood and bone marrow. Scand. J. Clin. Lab. Invest. 21:1968;1-89.
7. Condeelis J. Life at the leading edge: the formation of cell protrusions. Annu. Rev. Cell Biol. 9:1993;411-444.
8. Cross M.J., Stewart A., Hodkin M.N., Kerr D.J., Wakelam M.J.O. Wortmannin and its structural analogue demethoxyviridin inhibit stimulated phospholipase A2 activity in Swiss 3T3 cells. J. Biol. Chem. 270:1995;25352-25355.
9. Derman M.P., Chen J.Y., Spokes K.C., Songyang Z., Cantley L.G. An 11-amino acid sequence from C-met initiates epithelial chemotaxis via phosphatidylinositol 3-kinase and phospholipase C. J. Biol. Chem. 271:1996;4251-4255.
10. Devreotes P.N., Zigmond S.H. Chemotaxis in eukaryotic cells: a focus on leukocytes and Dictyostelium. Annu. Rev. Cell Biol. 4:1988;649-686.
11. Dewald B., Thelen M., Baggiolini M. Two transduction sequences are necessary for neutrophil activation by receptor agonists. J. Biol. Chem. 263:1988;16179-16184.
12. Eberle M., Traynor-Kaplan A.E., Sklar L.A., Norgauer J. Is there a relationship between phosphatidylinositoltrisphosphate and F-actin polymerization in human neutrophils. J. Biol. Chem. 265:1990;16725-16728.
13. Ferrante A., Thong Y.H. Optimal conditions for simultaneous purification of mononuclear and polymorphonuclear leucocytes from human blood by the Hypaque-Ficoll method. J. Immunol. Methods. 36:1980;109-117.
14. Fukami K., Furuhashi K., Inagaki M., Endo T., Hatano S., Takenawa T. Requirement of phosphatidylinositol 4,5-bisphosphate for α-actinin function. Nature. 359:1992;150-152.
15. Gaudry M., Caon A.C., Gilbert C., Lille S., Naccache P.H. Evidence for the involvement of tyrosine kinases in the locomotory responses of human neutrophils. J. Leuk. Biol. 51:1992;103-108.
16. Howard T.H., Meyer W.H. Chemotactic peptide modulation of actin assembly and locomotion in neutrophils. J. Cell. Biol. 98:1984;1265-1271.
17. Isenberg, G., Goldmann, W.H., 1995. Actin-binding proteins-lipid interactions. In: Hesketh, J.E., Pryme, J.F. (Eds.), The Cytoskeleton, vol. 1. Structure and Assembly. JAI Press Inc., London, England, pp. 169-204.
18. Keller H.U., Niggli V. Selective effects of the PKC inhibitors Ro 31-8220 and CGP 41 251 on PMN locomotion, cell polarity and pinocytosis. J. Cell. Physiol. 161:1994;526-536.
19. Keller H.U., Niggli V. Effects of cytochalasin D on shape and fluid pinocytosis in human neutrophils as related to cytoskeletal changes (actin, α-actinin and microtubules). Eur. J. Cell Biol. 66:1995;157-164.
20. Knall C., Worthen G.S., Johnson G.L. Interleukin 8-stimulated phosphatidylinositol-3-kinase activity regulates the migration of human neutrophils independent of extracellular signal-regulated kinase and p38 mitogen-activated protein kinases. Proc. Natl. Acad. Sci. USA. 94:1997;3052-3057.
21. Kotani K., Yonezawa K., Hara K., Ueda H., Kitamura Y., Sakaue H., Ando A., Chavanieu A., Calas B., Grigorescu F., Nishiyama M., Waterfield M.D., Kasuga M. Involvement of phosphoinositide 3-kinase in insulin- or IGF-1-induced membrane ruffling. EMBO J. 13:1994;2313-2321.
22. Kreienbühl P., Keller H.U., Niggli V. Protein phosphatase inhibitors okadaic acid and calyculin A alter cell shape and F-actin distribution and inhibit stimulus-dependent increases in cytoskeletal actin of human neutrophils. Blood. 80:1992;2911-2919.
23. 4. Nature. 227:1970;680-685.
24. Lepidi H., Zaffran Y., Ansaldi J.L., Mege J.L., Capo C. Morphological polarization of human polymorphonuclear leucocytes in response to three different chemoattractants: an effector response independent of calcium rise and tyrosine kinases. J. Cell Sci. 108:1995;1771-1778.
25. Lu P.-J., Shieh W.-R., Rhee S.G., Yin H.L., Chen C.-S. Lipid products of phosphoinositide 3-kinase bind human profilin with high affinity. Biochemistry. 35:1996;14027-14034.
26. 2+. Trends Cell Biol. 3:1993;386-391.
27. Metzner B., Heger M., Hofmann C., Czech W., Norgauer J. Evidence for the involvement of phosphatidylinositol 4,5-bisphosphate 3-kinase in CD18-mediated adhesion of human neutrophils to fibrinogen. Biochem. Biophys. Res. Commun. 232:1997;719-723.
28. Naccache P.H., Gilbert C., Caon A.C., Gaudry M., Huang C.-K., Bonak U.A., Umezawa K., McColl S.R. Selective inhibition of human neutrophil functional responsiveness by erbstatin, an inhibitor of tyrosine protein kinase. Blood. 76:1990;2098-2104.
29. Naccache P.H., Caon A.S., Gilbert C., Gaudry M., Roberge C.J., Poubelle P.E., Bourgoin S. Inhibition of tyrosine phosphorylation by wortmannin in human neutrophils. Lab. Invest. 69:1993;19-23.
30. Nakanishi S., Yano H., Matsuda Y. Novel functions of phosphatidylinositol 3-kinase in terminally differentiated cells. Cell Signal. 7:1995;545-557.
31. Niggli V., Jenni V. Actin-associated proteins in human neutrophils: identification and reorganization upon cell activation. Eur. J. Cell Biol. 49:1989;366-372.
32. Niggli V., Keller H.U. On the role of protein kinases in regulating neutrophil actin association with the cytoskeleton. J. Biol. Chem. 266:1991;7927-7932.
33. Niggli V., Keller H.U. Inhibition of chemotactic peptide-induced development of cell polarity by the protein kinase C inhibitor CGP 41 251 in human neutrophils correlates with inhibition of protein phosphorylation. Expt. Cell Res. 204:1993;346-355.
34. Okada K., Takano-Ohmuro H., Obinata T., Abe H. Dephosphorylation of cofilin in polymorphonuclear leukocytes derived from peripheral blood. Expt. Cell Res. 227:1996;116-122.
35. Pardee J.D., Spudich J.A. Purification of muscle actin. Methods Cell Biol. 24:1982;271-289.
36. Rao K.M.K., Varani J. Actin polymerization induced by chemotactic peptide and concanavalin in rat neutrophils. J. Immunol. 129:1982;1605-1607.
37. Reinhold S., Prescott S.M., Zimmermann G.A., McIntyre T.M. Activation of human neutrophil phospholipase D by three separable mechanisms. FASEB J. 4:1990;208-214.
38. Shibasaki F., Fukami K., Fukui Y., Takenawa T. Phosphatidylinositol 3-kinase binds to α-actinin through the p85 subunit. Biochem. J. 302:1994;551-557.
39. Stephens L., Hughes K., Irvine R.T. Pathway of phosphatidylinositol (3,4,5) trisphosphate synthesis in activated neutrophils. Nature. 351:1991;33-39.
40. Thelen M., Uguccioni M., Bösiger J. PI 3-kinase-dependent and independent chemotaxis of human neutrophil leukocytes. Biochem. Biophys. Res. Commun. 217:1995;1255-1262.
41. Traynor-Kaplan A.E., Thompson B.L., Harris A.L., Taylor P., Omann G.M., Sklar L.A. Transient increase in phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol trisphosphate during activation of human neutrophils. J. Biol. Chem. 264:1989;15668-15673.
42. Ui M., Okada T., Hazeki K., Hazeki O. Wortmannin as a unique probe for an intracellular signalling protein, phosphoinositide 3-kinase. Trends Biol. Sci. 20:1995;303-307.
43. Vlahos C.J., Matter W.F., Brown R.F., Traynor-Kaplan A.E., Heyworth P.G., Prossnitz E.R., Ye R.D., Marder P., Schelm J.A., Rothfuss K.J., Serlin B.S., Simpson P.J. Investigation of neutrophil signal transduction using a specific inhibitor of phosphatidylinositol 3-kinase. J. Immunol. 154:1995;2413-2422.
44. Watts R.G., Howard T.H. Mechanisms for actin reorganisation in chemotactic factor-activated polymorphonuclear leukocytes. Blood. 81:1993;2750-2757.
45. White J.R., Naccache P.H., Sha'afi R.I. Stimulation by chemotactic factor of actin association with the cytoskeleton in rabbit neutrophils. J. Biol. Chem. 258:1983;14041-14047.
46. Wymann M.P., Bulgarelli-Leva G., Zvelebil M.J., Pirola L., Vanhaesebroeck B., Waterfield M.D., Panatoyou G. Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction. Mol. Cell. Biol. 16:1996;1722-1733.
47. Zigmond S.H. Recent quantitative studies of actin filament turnover during cell locomotion. Cell Motil. Cytoskel. 25:1993;309-316.
48. Zimmermann A., Gehr P., Keller H.U. Diacylglycerol-induced shape changes, movements and altered F-actin distribution in human neutrophils. J. Cell Sci. 90:1988;651-666.