Pharmacological and molecular evidence for the expression of the two steroid 5α-reductase isozymes in normal and hyperplastic human prostatic cells in culture

Prostate

Volumn 32, Issue 3, 1997, Pages 155-163

  Berthaut, Isabelle ^a   Mestayer, Chidi ^b   Portois, Marie Claire ^a   Cussenot, Olivier ^c   Mowszowicz, Irène ^a  

^a HÔPITAL NECKER ENFANTS MALADES   (France)

^b PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^c SAINT LOUIS HOSPITAL   (France)

Author keywords

Isozyme expression; Normal and hyperplastic prostate; Prostate cells in culture; Steroid 5 reductases

Indexed keywords

ANDROSTANOLONE; EPRISTERIDE; FINASTERIDE; MESSENGER RNA; STEROID 5ALPHA REDUCTASE; TESTOSTERONE;

ARTICLE; CELL CULTURE; CELL IMMORTALIZATION; EMBRYO DEVELOPMENT; ENZYME ACTIVITY; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; PROSTATE EPITHELIUM; PROSTATE HYPERTROPHY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANDROSTADIENES; BASE SEQUENCE; BENZOQUINONES; BLOTTING, NORTHERN; CELL LINE; CELLS, CULTURED; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME INHIBITORS; EPITHELIAL CELLS; EPITHELIUM; FIBROBLASTS; FINASTERIDE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; ISOENZYMES; MALE; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; PROSTATE; PROSTATIC HYPERPLASIA; RNA, MESSENGER; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 0030816596     PISSN: 02704137     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0045(19970801)32:3<155::AID-PROS1>3.0.CO;2-K     Document Type: Article

References (40)

1. Coffey DS, Isaacs JT: The control of prostate growth. Urology 17 (Suppl):17-24, 1981.
2. Bruchovsky N, Wilson JD: The conversion of testosterone to 5α-androstane-17β-o1-3-one by rat prostate in vivo and in vitro. J Biol Chem 243:2012-2021, 1968.
3. Frederiksen DW, Wilson JD: Partial characterization of the nuclear reduced nicotinamide adenine dinucleotide phosphate Δ4-3-ketosteroid 5α-oxidoreductase of rat prostate. J Biol Chem 246:2584-2593, 1971.
4. Moore RJ, Wilson JD: Localization of the reduced nicotinamide adenine dinucleotide phosphate Δ4-3-ketosteroid 5α-oxidoreductase in the nuclear membrane of the rat ventral prostate. J Biol Chem 247:958-967, 1972.
5. Andersson S, Russell DW: Structure and biochemical properties of cloned and expressed human and rat steroid 5α-reductases. Proc Natl Acad Sci USA 87:3640-3644, 1990.
6. Andersson S, Berman DM, Jenkins EP, Russell DW: Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism. Nature 354:159-161, 1991.
7. Jenkins EP, Hsieh CL, Milatovich A, Normington K, Berman DM, Francke U, Russell DW: Characterization and chromosomal mapping of a human steroid 5α-reductase gene and pseudogene and mapping of the mouse homologue. Genomics 11:1102-1112, 1991.
8. Labrie F, Sugimoto Y, Luu-The V, Simard J, Lachance Y, Bachvarov D, Leblanc G, Durocher F, Paquet N: Structure of human type II 5α-reductase gene. Endocrinology 131:1571-1573, 1992.
9. Wigley WC, Prihoda JS, Mowszowicz I, Mendonca BB, New MI, Wilson JD, Russell DW: Natural mutagenesis study of the human 5α-reductase 2 isozyme. Biochemistry 33:1265-1270, 1994.
10. Jenkins EP, Andersson S, Imperato-McGinley J, Wilson JD, Russell DW: Genetic and pharmacological evidence for more than one human steroid 5α-reductase. J Clin Invest 89:293-300, 1992.
11. Wilson JD, Griffin JE, Russell DW: Steroid 5α-reductase 2 deficiency. Endocr Rev 14:577-593, 1993.
12. Normington K, Russell DW: Tissue distribution and kinetic characteristics of rat steroid 5α-reductase isozymes. J Biol Chem 267:19548-19554, 1992.
13. Berman DM, Russell DW: Cell-type-specific expression of rat steroid 5α-reductase isoenzymes. Proc Natl Acad Sci USA 90: 9359-9363, 1993.
14. Bonnet P, Reiter E, Bruyninx M, Sente B, Dombrowicz D, De Levai J, Closset J, Hennen G: Benign prostatic hyperplasia and normal prostate aging: Differences in types I and II 5α-reductase and steroid hormone receptor messenger ribonucleic acid (mRNA) levels, but not in insulin-like growth factor mRNA levels. J Clin Endocrinol Metab 77:1203-1208, 1993.
15. Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell JD, Russell DW: Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. J Clin Invest 92:903-910, 1993.
16. Silver RI, Wiley EL, Thigpen AE, Guileyardo JM, McConnell JD, Russell DW: Cell type specific expression of steroid 5α-reductase 2. J Urol 152:438-442, 1994.
17. Kaefer M, Audia JE, Bruchovsky N, Goode RL, Hsiao KC, Leibovitch IY, Krushinski JH, Lee C, Steidle CP, Sutkowski DM, Neubauer BL: Characterization of Type 1 5α-reductase activity in DU-145 human prostatic adenocarcinoma cells. J Steroid Biochem Mol Biol 58:195-205, 1996.
18. Gormley GJ, Stoner E, Bruskewitz RC, Imperato-McGinley J, Walsh PC, McConnell JD, Andriole GL, Geller J, Bracken BR, Tenover JS, Vaughan ED, Pappas F, Taylor A, Binkowitz B, Ng J: The effect of finasteride in men with benign prostatic hyperplasia. N Engl J Med 327:1185-1191, 1992.
19. Mowszowicz I, Bardin CW: In vitro androgen metabolism in mouse kidney: High 3 keto-reductase (3α-hydroxysteroid dehydrogenase) activity relative to 5α-reductase. Steroids 23:793-807, 1974.
20. Cussenot O, Berthon P, Berger R, Mowszowicz I, Faille A, Hojman F, Teillac P, Le Duc A, Calvo F: Immortalization of human adult normal prostatic epithelial cells by liposomes containing large T-SV40 gene. J Urol 143:881-886, 1991.
21. Mowszowicz I, Kirchhoffer MO, Kuttenn F, Mauvais-Jarvis P: Testosterone 5α-reductase activity of skin fibroblasts: Increase with serial subcultures. Mol Cell Endocrinol 17:41-50, 1980.
22. Berthaut I, Portois M-C, Cussenot O, Mowszowicz I: Human prostatic cells in culture: Different testosterone metabolic profile in epithelial cells and fibroblasts from normal or hyperplastic prostates. J Steroid Biochem Mol Biol 58:235-242, 1996.
23. Tian G, Stuard JD, Moss ML, Domanico PL, Bramson HN, Patel IR, Kadwell SH, Overton LK, Kost TA, Mook RA Jr., Frye SV, Batchelor KW, Wiseman JS: 17β-(N-tert-butylcarbamoyl)-4 aza-5α-androstan-1-en-3-one is an active site-directed slow time-dependent inhibitor of human steroid 5α-reductase 1. Biochemistry 33:2291-2296, 1994.
24. Levy MA, Brandt M, Sheedy KM, Dinh JT, Holt DA, Garrison LM, Bergsma DJ, Metcalf BW: Epristeride is a selective and specific uncompetitive inhibitor of human steroid 5α-reductase isoform 2. J Steroid Biochem Mol Biol 48:197-206, 1994.
25. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156-159, 1987.
26. Mestayer CH, Berthaut I, Portois M-C, Wright F, Kuttenn F, Mowszowicz I, Mauvais-Jarvis P: Predominant expression of 5α-reductase type 1 in pubic skin from normal subjects and hirsute women. J Clin Endocrinol Metab 81:1989-1993, 1996.
27. Burton K: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315-323, 1956.
28. Cunha GR, Chung LWK, Shannon JM, Taguchi O, Fujii H: Hormone-induced morphogenesis and growth: Role of mesenchymal-epithelial interactions. Rec Prog Horm Res 39:559-598, 1983.
29. Berthon P, Cussenot O, Hopwood L, Le Due A, Maitland NJ: Functional expression of SV 40 in normal human prostatic epithelial and fibroblastic cells: Differentiation pattern of non-tumorigenic cell lines. Int J Oncol 6:333-343, 1995.
30. Mc Neal JE: Origin and evolution of benign prostatic enlargement. Invest Urol 15:340-345, 1978.
31. Thigpen AE, Cala KM, Russell DW: Characterization of Chinese hamster ovary cell lines expressing human steroid 5α-reductase isoenzymes. J Biol Chem 268:17404-17412, 1993.
32. Luu-The V, Sugimoto Y, Puy L, Labrie Y, Solache IL, Singh M, Labrie F: Characterization, expression, and immunohistochemical localization of 5α-reductase in human skin. J Invest Dermatol 102:221-226, 1994.
33. Eicheler W, Tuohimaa P, Vilja P, Adermann K, Forssmann WG, Aumüller G: Immunocytochemical localization of human 5α-reductase 2 with polyclonal antibodies in androgen target and non-target tissues. J Histochem Cytochem 42:667-675, 1994.
34. Hirsch KS, Jones CD, Audia JE, Andersson S, McQuaid L, Stamm NB, Neubauer BL, Pennington P, Toomey RE, Russell DW: LY 191704: A selective, non steroidal inhibitor of human steroid 5α-reductase type 1. Proc Natl Acad Sci USA 90:5277-5281, 1993.
35. Delos S, Carsol J-L, Ghazarossian E, Raynaud J-P, Martin P-M: Testosterone metabolism in primary cultures of human prostate epithelial cells and fibroblasts. J Steroid Biochem Mol Biol 55: 375-383, 1995.
36. Russell DW, Wilson JD: Steroid 5α-reductase: Two genes/two enzymes. Annu Rev Biochem 63:25-61, 1994.
37. ®) in epithelium and stroma of human benign prostatic hyperplasia. Steroids 59:616-620, 1994.
38. Liang T, Cascieri MA, Cheung AH, Reynolds GF, Rasmusson GH: Species differences of prostatic 5α-reductases of rat, dog and human. Endocrinology 117:571-579, 1985.
39. Thigpen AE, Russell DW: Four-amino acid segment in steroid 5α-reductase 1 confers sensitivity to finasteride, a competitive inhibitor. J Biol Chem 267:8577-3583, 1992.
40. Faller B, Farley D, Nick H: Finasteride: A slow-binding 5α-reductase inhibitor. Biochemistry 32:5705-5710, 1993.