Predominant expression of 5α-reductase type 1 in pubic skin from normal subjects and hirsute patients

Journal of Clinical Endocrinology and Metabolism

Volumn 81, Issue 5, 1996, Pages 1989-1993

  Mestayer, Ch ^a   Berthaut, I ^a   Portois, M C ^a   Wright, F ^a   Kuttenn, F ^a   Mowszowicz, I ^a,b   Mauvais Jarvis, P ^a  

^a PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^b HÔPITAL NECKER ENFANTS MALADES   (France)

Author keywords

[No Author keywords available]

Indexed keywords

FINASTERIDE; LY 306089; MESSENGER RNA; STEROID 5ALPHA REDUCTASE; STEROID 5ALPHA REDUCTASE INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ISOLATION; ENZYME RELEASE; FEMALE; HIRSUTISM; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HUMAN TISSUE; MALE; MOLECULAR CLONING; NORMAL HUMAN; PRIORITY JOURNAL; PUBIS SYMPHYSIS; RNA ANALYSIS; SKIN FIBROBLAST;

BLOTTING, NORTHERN; ENZYME INHIBITORS; FEMALE; FIBROBLASTS; GENE EXPRESSION; GENITALIA; HIRSUTISM; HUMANS; MALE; POLYMERASE CHAIN REACTION; PUBIC BONE; PUBIC SYMPHYSIS; RNA, MESSENGER; SKIN; TESTOSTERONE 5-ALPHA-REDUCTASE;

EID: 0029884372     PISSN: 0021972X     EISSN: None     Source Type: Journal    
DOI: 10.1210/jc.81.5.1989     Document Type: Article

References (28)

1. Bruchovsky N, Wilson JD. 1968 The conversion of testosterone to 5α-androstane-17β-ol-3-one by rat prostate in vivo and in vitro. J Biol Chem. 243:2012-2021.
2. Mauvais-Jarvis P, Mowszowicz I, Kuttenn F. 1984 Significance of 5α-reductase activity in human sexual differentiation. In: Serio M, Motta M, Zanisi M, Martini L, eds. Sexual differentiation. Basic and clinical aspects. Serono Symposium. New York: Raven Press; vol 11:247-260.
3. Peterson RE, Imperato-McGinley J, Gautier T, Sturla E. 1977 Male pseudohermaphroditism due to steroid 5α-reductase deficiency. Am J Med 62:170-191.
4. Kuttenn F, Mauvais-Jarvis P. 1975 Testosterone 5α-reduction in the skin of normal subjects and of patients with abnormal sex development. Acta Endocrinol (Copenh). 79:164-176.
5. Kuttenn F, Mowszowicz I, Mauvais-Jarvis P. 1980 Androgen metabolism in human skin. In: Mauvais-Jarvis P, Vickers CFH, Wepierre J, eds. Percutaneous absorption of steroids. London: Academic Press; 99-121.
6. Mowszowicz I, Melanitou E, Kirchhoffer MO, Mauvais-Jarvis P. 1983 Dihydrotestosterone stimulates 5α-reductase activity in pubic skin fibroblasts. J Clin Endocrinol Metab. 56:320-325.
7. Andersson S, Bischop RW, Russell DW. 1989 Expression, cloning and regulation of steroid 5α-reductase, an enzyme essential for male sexual differentiation. J Biol Chem. 264:16249-16255.
8. Andersson S, Berman DM, Jenkins EP, Russell DW. 1991 Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism. Nature. 354:159-161.
9. Wilson JD, Griffin JE, Russell DW. 1993 Steroid 5α-reductase deficiency. Endocr Rev. 14:577-593.
10. Harris G, Azzolina B, Baginsky W, et al. 1992 Identification and selective inhibition of an isozyme of steroid 5α-reductase in human scalp. Proc Natl Acad Sci USA. 89:10787-10791.
11. Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell JD, Russell DW. 1993 Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. J Clin Invest. 92:903-910.
12. Mowszowicz I, Kirchhoffer MO, Kuttenn F, Mauvais-Jarvis P. 1980 Testosterone 5α-reductase activity of skin fibroblasts: increase with serial subculture. Mol Cell Endocrinol. 17:41-50.
13. Mowszowicz I, Melanitou E, Doukani A, Wright F, Kuttenn F, Mauvais-Jarvis P. 1983 Androgen binding capacity and 5α-reductase activity in pubic skin fibroblasts from hirsute patients. J Clin Endocrinol Metab. 56:1209-1213.
14. Tian G, Stuart JD, Moss ML, et al. 1994 17β-(N-Tert-butylcarbamoyl)-4-aza-5α-androstan-1-en-3-one is an active site-directed slow time-dependent inhibitor of human steroid 5α-reductase 1. Biochemistry. 33:2291-2296.
15. Kaefer M, Audia JE, Bruchovsky N, et al. 1995 Characterization of type 1 5α-reductase activity in DU-145 human prostatic adenocarcinoma cells. J Steroid Biochem Mol Biol. In press.
16. Chomczynski P, Sacchi N. 1987 Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:156-159.
17. Mestayer C, Gabou L, Portois MC, Boucekkine C, Chang C, Mowszowicz I. 1991 Expression du gène du récepteur des androgènes chez des sujets normaux et des patients présentant une insensibilité complète aux androgènes. Ann Endocrinol (Paris). 52:431-434.
18. Jenkins EP, Hsieh CL, Milatovich A, et al. 1991 Characterization and chromosomal mapping of a human 5α-reductase gene and pseudogene and mapping of the mouse homologue. Genomics. 11:1102-1112.
19. Labrie F, Sugimoto Y, Luu-The V, et al. 1992 Structure of human type II 5α-reductase gene. Endocrinology. 131:1571-1573.
20. Burton K. 1956 A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 62:315-323.
21. Kuttenn F, Mowszowicz I, Schaison G, Mauvais-Jarvis P. 1977 Androgen production and skin metabolism in hirsutism. J Endocrinol. 75:83-91.
22. Thigpen AE, Cala KM, Russell DW. 1993 Characterization of Chinese hamster ovary cell lines expressing human steroid 5α-reductase isozymes. J Biol Chem. 268:17404-17412.
23. Mowszowicz I, Riahi M, Wright F, Bouchard Ph, Kuttenn F, Mauvais-Jarvis P. 1981 Androgen receptor in human skin cytosol. J Clin Endocrinol Metab. 52:338-344.
24. Luu-The V, Sugimoto Y, Puy L, et al. 1994 Characterization, expression and immunohistochemical localization of 5α-reductase in human skin. J Invest Dermatol. 102:221-226.
25. Mauvais-Jarvis P, Kuttenn F, Mowszowicz I, Wright F. 1981 Different aspects of 5α-reductase deficiency in male pseudohermaphroditism and hypothyroidism. Clin Endocrinol (Oxf). 14:459-469.
26. Hirsch KS, Jones CD, Audia JE, et al. 1993 LY191704: a selective, nonsteroidal inhibitor of human steroid 5α-reductase type 1. Proc Natl Acad Sci USA. 90:5277-5281.
27. Compel A, Kuttenn F, Wright F, Mauvais-Jarvis P. 1986 Contribution of plasma androstenedione to 5α-androstanediol glucuronide in women with idiopathic hirsutism. J Clin Endocrinol Metab. 62:441-444.
28. Wright F, Mowszowicz I, Mauvais-Jarvis P. 1978 Urinary 5α-androstane-3α,17β-diol radioimmunoassay: a new clinical evaluation. J Clin Endocrinol Metab. 47:850-854.