Engineering of porcine pepsin: Alteration of S1 substrate specificity of pepsin to those of fungal aspartic proteinases by site-directed mutagenesis

Journal of Biological Chemistry

Volumn 272, Issue 30, 1997, Pages 18855-18861

  Shintani, Takahiro ^a,b   Nomura, Kouji ^a   Ichishima, Eiji ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b JAPAN SOCIETY FOR THE PROMOTION OF SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC PROTEINASE; MICROBIAL ENZYME; MUTANT PROTEIN; PEPSIN A;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME PURIFICATION; ENZYME SPECIFICITY; GENETIC ENGINEERING; PRIORITY JOURNAL; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; SWINE;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; ASPARTIC ENDOPEPTIDASES; CATTLE; FUNGAL PROTEINS; GLYCINE; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; LYSINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPSIN A; PROTEIN ENGINEERING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SERINE; SUBSTRATE SPECIFICITY; SWINE; THREONINE;

BOVINAE; SUIDAE; SUS SCROFA;

EID: 0030795330     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.30.18855     Document Type: Article

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