Characterization of the S1 subsite specificity of aspergillopepsin I by site-directed mutagenesis

Journal of Biochemistry

Volumn 120, Issue 5, 1996, Pages 974-981

  Shintani, Takahiro ^a   Kobayashi, Mizue ^a   Ichishima, Eiji ^a  

^a TOHOKU UNIVERSITY   (Japan)

Author keywords

Aspartic proteinase; Aspergillopepsin I; Site directed mutagenesis; Substrate specificity; Trypsinogen activation

Indexed keywords

ASPARTIC ACID; ASPARTIC PROTEINASE; SERINE; THREONINE; ASPERGILLOPEPSIN I; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; NONHUMAN; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; CIRCULAR DICHROISM; COMPARATIVE STUDY; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN CONFORMATION;

ASPERGILLUS; ASPERGILLUS SAITOI; ESCHERICHIA COLI; MAMMALIA;

AMINO ACID SEQUENCE; ASPARTIC ENDOPEPTIDASES; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 0030275785     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021515     Document Type: Article

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