Structure and role in symbiosis of the exoB gene of Rhizobium leguminosarum bv trifolii

Molecular and General Genetics

Volumn 255, Issue 2, 1997, Pages 131-140

  Sanchez Andujar B ^a   Coronado, C ^a   Philip Hollingsworth, S ^b   Dazzo, F B ^c   Palomares, A J ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

^b Michigan State University ^*  (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

exoB gene; Exopolysaccharide; Rhizobium leguminosarum bv trifolii; Trifolium repens; Trifolium subterraneum

Indexed keywords

DNA FRAGMENT; GALACTOSE; LIPOPOLYSACCHARIDE; OLIGOSACCHARIDE; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; BACTERIAL GENE; GENE MUTATION; GENE STRUCTURE; GENETIC COMPLEMENTATION; NONHUMAN; OPEN READING FRAME; PLANT CELL; PRIORITY JOURNAL; RHIZOBIUM LEGUMINOSARUM; RHIZOBIUM TRIFOLII; SYMBIOSIS;

CARBOHYDRATE SEQUENCE; CLONING, MOLECULAR; FABACEAE; GENES, BACTERIAL; MOLECULAR SEQUENCE DATA; MUTATION; PLANTS, MEDICINAL; POLYSACCHARIDES, BACTERIAL; RESTRICTION MAPPING; RHIZOBIUM LEGUMINOSARUM; SEQUENCE HOMOLOGY, NUCLEIC ACID; SINORHIZOBIUM MELILOTI; SYMBIOSIS; UDPGLUCOSE 4-EPIMERASE;

BACTERIA (MICROORGANISMS); RHIZOBIUM LEGUMINOSARUM; TRIFOLIUM REPENS; TRIFOLIUM SUBTERRANEUM;

EID: 0030794388     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050481     Document Type: Article

References (48)

1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seichnan JG, Smith JA, Struhl F (1989) Current protocols in molecular biology. Greene and Wiley Interscience, New York
2. Beringer JE (1974) R-factor transfer in Rhizobium leguminosarum. J Gen Microbiol 84:188-198
3. Bhagwat AA, Tully RE, Kesister DL (1991) Isolation and characterization of a competition-defective Bradyrhizobium japonicum mutant. Appl Environ Microbiol 57:3496-3501
4. Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of restriction and modification in Escherichia coli. J Mol Biol 41:459-472
5. Breedveld MW, Canter Cremers HCJ, Batley M, Posthumus MA, Zevenhuizen LPTM, Wijffelman CA, Zehnder AJB (1993) Polysaccharide synthesis in relation to nodulation behavior of Rhizobium leguminosarum. J Bacteriol 175:750-757
6. Buendía AM, Enenkel B, Köplin R, Niehaus K, Arnold W, Pühler A (1991) The Rhizobium meliloti exoZ/exoB fragment of megaplasmid 2: ExoB functions as a UDP-glucose 4-epimerase and ExoZ shows homology to NodX of Rhizobium leguminosarum biovar viciae strain TOM. Mol Microbiol 5:1519-1530
7. Cangelosi GA, Hung L, Puvanesarajah V, Stacey G, Ozga DA, Leigh JA, Nester EW (1987) Common loci for Agrobacterium tumefaciens and Rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. J Bacteriol 169:2086-2091
8. Canter Cremers HCJ, Batley M, Redmond JW, Eydems L, Breedveld MW, Zevenhuizen LPTM, Pees E, Wijffelman CA, Lugtenberg BJJ (1990) Rhizobium leguminosarum exoB mutants are deficient in the synthesis of UDP-glucose 4′ epimerase. J Biol Chem 265:21122-21127
9. Carlson RW (1984) The heterogeneity of Rhizobium lipopolysaccharides. J Bacteriol 158:1012-1017
10. Chakravorty A, Zurkowski W, Shine J, Rolfe BG (1982) Symbiotic nitrogen fixation: molecular cloning of Rhizobium genes involved in exopolysaccharide synthesis and effective nodulation. J Mol Appl Genet 1:585-596
11. Corbin D, Ditta GS, Helinski DR (1982) Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti. J Bacteriol 149:221-228
12. Coronado C, Sanchez-Andujar B, Palomares AJ (1996) Rhizobium extracellular structures in symbiosis. World Microbiol Biotechnol 12:127-136
13. Dazzo FB (1982) Leguminous root nodules. In: Burns RG, Slater JH (eds) Experimental microbial ecology. Blackwell Scientific Publications, Oxford, pp 431-446
14. De Lorenzo V, Herrero M, Jakubzik U, Timmis UK (1990) MiniTn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172:6568-6572
15. Derylo M, Skorupska A, Bednara J, Lorkiewicz Z (1986) Rhizobium trifolii mutants deficient in exopolysaccharide production. Physiol Plant 66:699-704
16. De Troch P, Keijers V, Vanderleyden J (1994) Sequence analysis of the Azospirillum brasilense exoB gene, encoding UDP-glucose 4′-epimerase. Gene 144:143-144
17. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395
18. Diebold R, Noel KD (1989) Rhizobium leguminosarum exopolysaccharide mutants: biochemical and genetic analyses and symbiotic behaviour on three hosts. J Bacteriol 171:4821-4830
19. Ditta G (1986). Tn5 mapping of Rhizobium nitrogen fixation genes. Methods Enzymol 118:519-528
20. Ditta G, Stanfield S, Corbin D, Helinski DR (1980). Broad host-range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77:7347-7351
21. Ditta G, Schmidhauser T, Yakobson E, Lu P, Liang XW, Finlay DR, Guiney D, Helinski DR (1985) Plasmid related to the broad host-range vector, pRK290, useful for gene cloning and for monitoring gene expression. Plasmid 13:149-153
22. Eckhardt T (1978) A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid 1:584-588
23. Henikoff S (1984) Unidirectional digestions with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351-359
24. Hollingsworth R, Abe M, Sherwood J, Dazzo FB (1984) Bacteriophage-induced acidic heteropolysaccharide lyases that convert the acidic heteropolysaccharides of Rhizobium trifolii into oligosaccharide units. J Bacteriol 160:510-516
25. Hollingsworth R, Carlson RW, Garcia F, Gage DA (1989) A new core tetrasaccharide component from the lipopolysaccharide from Rhizobium trifolii ANU843. J Biol Chem 264:9300-9304
26. Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989-2998
27. Kittelberger R, Hilbink F (1993) Sensitive silver-staining detection of bacterial lipopolysaccharides in polyacrylamide gels. J Biochem Biophys Methods 26:81-86
28. Kondorosi A, Kondorosi E, Pankhurst CE, Broughton W, Banfalvi S (1982) Mobilization of a Rhizobium meliloti megaplasmid carrying nodulation and nitrogen fixation genes into other rhizobia and Agrobacterium. Mol Gen Genet 188:433-439
29. Köplin R, Wang G, Hötte B, Priefer UB, Pühler A (1993) A 3.9 kb DNA region of Xanthomonas campestris pv campestris that is necessary for lipopolysaccharide production encodes a set of enzymes involved in the synthesis of dTDP-rhamnose. J Bacteriol 175:7786-7792
30. Leigh JA, Lee CT (1988) Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules. J Bacteriol 170:3327-3332
31. Leigh JA, Walker GC (1994) Exopolysaccharides of Rhizobium: synthesis, regulation and symbiotic function. Trends Genet 10: 63-67
32. Leigh JA, Signer ER, Walker GC (1985) Exopolysaccharide-deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc Natl Acad Sci USA 82:6231-6235
33. Lemaire HG, Müller-Hill B (1986) Nucleotide sequences of the galE gene and the galT gene of E. coli. Nucleic Acids Res 14:7705-7711
34. Long S, Reed JW, Himawan J, Walker GC (1988) Genetic analysis of a cluster of genes required for synthesis of the Calcofluor-binding exopolysaccharides of Rhizobium meliloti. J Bacteriol 170:4239-4248
35. Lopez-Lara IM, Orgambide G, Dazzo FB, Olivares J, Toro N (1995) Surface polysaccharide mutants of Rhizobium sp. (Acacia) strain GRH2: major requirement of lipopolysaccharide for successful invasion of Acacia nodules and host range determination. Microbiology 141:573-581
36. Meade HM, Long SR, Ruvkun GB, Brown SE, Ausubel FM (1982) Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol 149:114-122
37. Megias M, Caviedes MA, Andres M, Sousa C, Ruiz-Berraquero F, Palomares AJ (1988) Localization of his genes on the Rhizobium trifolii RS800 linkage map. Mol Gen Genet 211:369-372
38. Ollero FJ, Valverde MA, Sánchez-Palazón L, Villalobo E, Espuny MR, Bellogín R (1994) An exoB mutant of Rhizobium sp. is effective in indeterminate nodules of Hedysarum coronarium. Microbiology 140:1389-1394
39. Orosz L, Svab Z, Kondorosi A, Silk T (1973) Genetic studies on Rhizobiophage 16-3. I. Genes and functions on the chromosome. Mol Gen Genet 125:341-350
40. Philip-Hollingsworth S, Hollingsworth R, Dazzo FB (1989) Host-range related structural features of the acidic extracellular polysaccharides of Rhizobium trifolii and Rhizobium leguminosarum. J Biol Chem 264:5710-5714
41. Quandt J, Hynes MF (1993) Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127:15-21
42. Rodriguez-Quiñones F, Fernandez-Burriel M, Banfalvi Z, Megias M, Kondorosi A (1989) Identification of a conserved, reiterated DNA region that influences the efficiency of nodulation in strain RS1051 of Rhizobium leguminosarum by trifolii. Mol. Plant-Microbe Interact 2:75-23
43. Rolfe BG, Gresshoff PM, Shine J, Vincent JW (1980) Interaction between a non-nodulating and an ineffective mutant of Rhizobium trifolii resulting in effective (nitrogen fixing) nodulation. Appl Environ Microbiol 39:449-452
44. Rosenberg C, Casse-Delbart F, Dusha I, David M, Boucher C (1982) Megaplasmid in the plant-associated bacteria Rhizobium meliloti and Pseudomonas solanacearum. J Bacteriol 150:402-406
45. Simon R, Quandt J, Klipp W (1989) New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in gram-negative bacteria. Gene 80:161-169
46. Yang C, Signer ER, Hirsch AM (1992) Nodules initiated by Rhizobium meliloti exopolysaccharide mutants lack a discrete, persistent nodule meristem. Plant Physiol 98:143-151
47. Yanisch-Perron C, Vieira J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13mp18 and pUC19 vectors. Gene 33:103-119
48. Zevenhuizen LPTM, Bertocchi C, Van Neerven ARW (1986) Congo red absorption and cellulose synthesis by Rhizobiaceae, Antonie Van Leeuwenhoek 52:381-386