Immunohistochemical differentiation of monoclonal GAD antibodies recognizing linear or conformational epitope regions

Pancreas

Volumn 15, Issue 2, 1997, Pages 139-146

  Augstein, Petra ^a   Ziegler, Brigitte ^a   Schlosser, Michael ^a   Flassig, Steffen ^a   Strebelow, Martina ^a   Ziegler, Manfred ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

Author keywords

GAD epitopes; GAD65; Glutamic acid decarboxylase (GAD) antibodies; Insulin dependent diabetes mellitus; Stiff man syndrome

Indexed keywords

AUTOANTIBODY; EPITOPE; GLUTAMATE DECARBOXYLASE; MONOCLONAL ANTIBODY;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; ANTIBODY TITER; ANTIGEN RECOGNITION; ARTICLE; AUTOIMMUNE DISEASE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; HUMAN TISSUE; INSULIN DEPENDENT DIABETES MELLITUS; MALE; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL; SCHOOL CHILD; STIFF MAN SYNDROME;

EID: 0030786741     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006676-199708000-00005     Document Type: Article

References (31)

1. Baekkeskov S, Aanstoot H-J, Christgau S, et al. Identification of the 64 K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature 1990;7: 151-6.
2. Erdo SL, Wolff JR. Gamma-aminobutyric acid outside the mammalian brain. J Neurochem 1990;54:363-72.
3. Bu DF, Erlander MG, Hitz BC, et al. Two human glutamate decarboxylases. GAD65 and GAD67, are each encoded by a single gene. Proc Natl Acad Sci USA 1992;89:2115-9.
4. Erlander MG, Tillakaratne NJK, Feldblum S, Patel N, Tobin AJ. Two genes encode distinct glutamate decarboxylases. Neuron 1991;7:91-100.
5. Solimena M, Folli F, Denis-Donini S, et al. Autoantibodies to glutamic acid decarboxylase in a patient with Stiff-man syndrome, epilepsy, and Type I diabetes mellitus. N Engl J Med 1988;318: 1012-20.
6. Solimena M, Butler MH, De Camilli P. GAD, diabetes, and stiffman syndrome: some progress and more questions. J Endocrinol Invest 1994;17:509-20.
7. Hagopian WA, Michelsen B, Karlsen AE, et al. Autoantibodies in IDDM primarily recognize the 65,000-Mr rather than the 67,000-Mr isoform of glutamic acid decarboxylase. Diabetes 1993;42: 631-6.
8. Lühder F, Schlosser M, Mauch L, et al. Autoantibodies against GAD65 rather than GAD67 predict the onset of Type 1 diabetes. Autoimmunity 1994;19:71-80.
9. Daw K, Powers AC. Two distinct glutamic acid decarboxylase auto-antibody specificities in IDDM target different epitopes. Diabetes 1995;44:216-20.
10. Thai A-C, Eisenbarth GS. Natural history of IDDM. Diabetes Rev 1993;1:1-14.
11. Björk E, Velloso LA, Kämpe O, Karlsson FA. GAD autoantibodies in IDDM, Stiff-man syndrome, and autoimmune polyendocrine syndrome type I recognize different epitopes. Diabetes 1994;43: 161-5.
12. Butler MH, Solimena M, Dirkx R, Haysay A, De Camilli P. Identification of a dominant epitope of glutamic acid decarboxylase (GAD-65) recognized by autoantibodies in stiff-man syndrome. J Exp Med 1993;178:2097-106.
13. Atkinson MA, Kaufman DL, Newman D, Tobin AJ, Maclaren NK. Islet cell cytoplasmic autoantibody reactivity to glutamate decarboxylase in insulin-dependent diabetes. J Clin Invest 1993;91: 350-6.
14. Bottazzo GF, Florin-Christensen A, Doniach D. Islet cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet 1974;2:1279-83.
15. Richter W, Seissler J, Northemann W, Wolfahrt S, Meinck HM, Scherbaum WA. Cytoplasmic islet cell antibodies recognize distinct islet antigens in IDDM but not in stiff man syndrome. Diabetes 1993;42:1642-8.
16. Kim J, Namchuk M, Bugawan T, et al. Higher autoantibody levels and recognition of a linear NH2-terminal epitope in the autoantigen GAD65, distinguish stiff-man syndrome from insulin-dependent diabetes mellitus. J Exp Med 1994;180:595-606.
17. Richter W, Shi Y, Baekkeskov S. Autoreactive epitopes in glutamic acid decarboxylase defined by diabetes-associated autoantibodies. Proc Natl Acad Sci USA 1993;90:2832-6.
18. Ziegler B, Augstein P, Schröder D, et al. Glutamate decarboxylase (GAD) is not detectable on the surface of rat islet cells examined by cytofluorometry and complement-dependent antibody-mediated cytotoxicity of monoclonal GAD antibodies. Horm Metab Res 1996;28:11-5.
19. Schlosser M, Witt S, Ziegler B, Ziegler M. Influence of target cell preparation on binding of monoclonal islet cell reactive antibodies (mc-ICRA) in cellular enzyme-linked immunosorbent assay (CELISA). J Immunol Methods 1991;140:101-9.
20. Augstein P, Ziegler B, Schlosser M, Klöting I, Ziegler M. Occurrence of beta-cell specific antibodies in diabetes prone BB/OK rats is not associated with the autoimmune progressive loss of beta cells: a study with monoclonal antibodies. Diabetes Res 1993;23: 139-51.
21. Ziegler B, Augstein P, Lühder F, et al. Monoclonal antibodies specific to glutamic acid decarboxylase 65 kDa isoform derived from a non-diabetic non-obese diabetic (NOD) mouse after immunization by Freund's adjuvant and a single dose of streptozotocin. Diabetes Res 1994;25:47-64.
22. Ziegler B, Schlosser M, Strebelow M, et al. Autoimmune-dominant GAD65 epitope regions are recognized by monoclonal antibodies generated in Balb/c mice. Autoimmunity 1995;21:38(A144).
23. Schlosser M, Hahmann J, Augstein P, Ziegler B, Ziegler M. A sensitive ELISA for quantitative determination of glutamate decarboxylase (GAD65). Diabetologia 1995;38:A6.
24. Vieregge P, Branczyk B, Barnett W, Stöcker W, Soyka D, Kömpf D. Stiff-man-Syndrom. Nervenarzt 1994;65:712-7.
25. Schlosser M, Hahmann J, Lühder F, Strebelow M, Ziegler M. Radioiodination of the 65kD and 67kD isoforms of glutamic acid decarboxylase (GAD) for application in radioimmunoassay. Diabetes Res 1995;29:17-31.
26. Mauch L, Abney CC, Berg H, Scherbaum WA, Liedvogel B, Northemann W. Characterization of a linear epitope within the human pancreatic 65-kDa glutamic acid decarboxylase and its autoimmune recognition by sera from insulin-dependent diabetes mellitus. Eur J Biochem 1993;212:597-603.
27. Nayak RC, Omar MAK, Rabizadeh A, Srikanta S, Eisenbarth GS. "Cytoplasmic" islet cell antibodies. Evidence that the target antigen is a sialoglycoconjugate. Diabetes 1985;34:617-9.
28. Rabin DU, Pleasic SM, Shapiro JA, et al. Islet cell antigen 512 is a diabetes-specific islet autoantigen related to protein tyrosine phosphatases. J Immunol 1994;152:3183-8.
29. Sai P, Elmansour A, Audrain M, Charbonnel B, Bardet S. Quantification of human cytoplasmic islet-cell antibodies which cross-react with mouse pancreas: a follow-up study in Type 1 (insulin-dependent) diabetic patients and in first degree relatives. Diabetologia 1993;36:778-84.
30. Genovese S, Bonifacio E, McNally J, et al. Distinct cytoplasmic islet cell antibodies with different risk for Type I (insulin-dependent) diabetes mellitus. Diabetologia 1992;35:385-8.
31. Kim J, Richter W, Aanstoot HJ, et al. Differential expression of GAD65 and GAD67 in human, rat and mouse pancreatic islets. Diabetes 1993;42:1799-808.