Glutamate Decarboxylase (GAD) is not Detectable on the Surface of Rat Islet Cells Examined by Cytofluorometry and Complement-Dependent Antibody-Mediated Cytotoxicity of Monoclonal GAD Antibodies

Hormone and Metabolic Research

Volumn 28, Issue 1, 1996, Pages 11-15

  Ziegler, Brigitte ^a   Augstein, Petra ^a   Schroder D ^a   Mauch, L ^b   Hahmann, J ^a   Schlosser, M ^a   Ziegler, M ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b ELIAS   (Germany)

Author keywords

Complement dependent antibody mediated cytotoxicity; Glutamic acid decarboxylase isoforms; Immunostaining; Monoclonal antibodies; Rat islet cells

Indexed keywords

GLUTAMATE DECARBOXYLASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOIMMUNITY; CONTROLLED STUDY; CYTOFLUOROMETRY; CYTOTOXICITY; FEMALE; INSULIN DEPENDENT DIABETES MELLITUS; MOUSE; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CHROMIUM RADIOISOTOPES; COMPLEMENT SYSTEM PROTEINS; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; GLUTAMATE DECARBOXYLASE; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN G; ISLETS OF LANGERHANS; MEMBRANE GLYCOPROTEINS; MICE; MICE, INBRED BALB C; RATS;

EID: 0030033089     PISSN: 00185043     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2007-979121     Document Type: Article

References (21)

1. Aguilar-Diosdado, R. L. D. Parkinson, J. A. Corbett, G. Kwon, C. A. Marshall, R. L. Gingerich, J. V. Santiago, M. L. McDaniel: Potential autoantigens in IDDM. Expression of carboxypeptidase-H and insulin but not glutamate decarboxylase on the β-cell surface. Diabetes 43: 418-425 (1994)
2. Atkinson, M. A., D. L. Kaufman, L. Campbell, K. A. Gibbs, S. C. Shah, D. F. Bu, M. G. Erlander, A. J. Tobin, N. K. MacLaren: Response of peripheral blood mononuclear cells to glutamate decarboxylase in insulin dependent diabetes. Lancet 339: 458-459 (1992)
3. Baekkeskov, S., J. Nielsen, B. Marner, T. Bilde, J. Ludvigsson, A. Lernmark: Autoantibodies in newly diagnosed diabetic children immunoprecipitate human pancreatic islet-cell proteins. Nature (Lond) 298: 167-169 (1982)
4. Baekkeskov, S., H. J. Aanstoot, S. Christgau, A Reetz, M. Solimena, M. Cascalho, F. Folli, H. Richter-Olesen, P. De Camilli: Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature (Lond) 347: 151-156 (1990)
5. Bottazzo, G. F., A. Florin-Christensen, D. Doniach: Islet cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet 2: 1279-1283 (1974)
6. Castano, L., G. S. Eisenbarth: Type I diabetes: a chronic autoimmune disease of human, mouse and rat. Annu. Rev. Immunol. 8: 647-679 (1990)
7. Erlander, M. G., N. J. K. Tillakaratne, S. Feldblum, N. Patel, A. J. Tobin: Two genes encode distinct glutamate decarboxylase. Neuron 7: 91-100 (1991)
8. Kaufman, D. L., M. Clare-Salzler, J. D. Tianet, J. Tian, T. Forsthuber, G. S. P. Tinge, P. Robinson, M. A. Atkinson, E. E. Sercarz, A. J. Robin, P. V. Lehmann: Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin dependent diabetes. Nature 366: 69-72 (1993)
9. Kim, J., W. Richter, H. J. Aanstoot, Y. Shi, Q. Fu, R. Rajotte, G. Warnock, S. Baekkeskov: Differential expression of GAD65 and GAD67 in human, rat and mouse pancreatic islets. Diabetes 42: 1799-1808 (1993)
10. Lane, R. D.: A short-duration polyethylene glycol fusion technique for increasing production of monoclonal antibody-secreting hybridomas. J. Immunol. Meth. 81: 223-228 (1985)
11. Lühder, F., K.-P. Woltanski, J. Hamann, I. Klöting, B. Ziegler, M. Ziegler: Detection of antibodies against both isoforms of glutamate decarboxylase in BB/OK rats by western blotting and immuno trapping enzyme activity assay. Diabetes Res. 20: 97-107 (1992)
12. Lühder, F., K.-P. Woltanski, L. Mauch, H. Haubruck, I. Rjasanowski, D. Michaelis, M. Ziegler: Detection of autoantibodies to the 65 kDa form of glutamate decarboxylase by radioimmunoassay. Diabetologia 36 (Suppl. 1): A92 (1993)
13. Mauch, L., J. Seissler, H. Haubruck, N. J. Book, C. C. Abney, H. Berthold, C. Wirbelauer, B. Liedvogel, W. Scherbaum, W. Northemann: Baculovirus-mediated expression of human 65-kDa and 67-kDa glutamic acid decarboxylases in SF9 insect cells and their relevance in diagnosis of insulin-dependent diabetes mellitus. J. Biochem. 113: 699-704 (1993)
14. 65 antibodies in diabetes and other autoimmune diseases using a simple radioligand assay. Diabetes 43: 459-467 (1994)
15. Schlosser, M., S. Witt, B. Ziegler, M. Ziegler: Influence of target cell preparation on binding of monoclonal islet cell reactive antibodies (mc-ICRA) in cellular enzyme-linked immunosorbent assay (CELISA). J. Immunol. Meth. 140: 101-109 (1991)
16. Schröder, D., S. Schmidt, I. Klöting, B. Hehmke: Effect of syngeneic islet antigen administration on complement-dependent antibody-mediated cytotoxicity to islet cells and diabetes onset in diabetes-prone BB/OK rats. Autoimmunity 14: 283-289 (1993)
17. Tisch, R, X. D. Yang, S. M. Singer, R. S. Liblau, L. Fugger, H. O. McDevitt: Immune response to glutamic acid decarboxylase correlates with insulitis in non-obese diabetic mice. Nature 366: 72-75 (1993)
18. Velloso, L. A., O. Kämpe, D. L. Eizirik, A. Hallberg, A. Andersson, F. A. Karlsson: Human autoantibodies react with glutamic acid decarboxylase antigen in human and rat but not in mouse pancreatic islets. Diabetologia 36: 46 (1993)
19. Wu, J.-Y., T. Matsuda, E. Roberts: Purification and characterization of glutamate decarboxylase from mouse brain. J. Biochem. 248: 3029-3034 (1973)
20. Ziegler, B., S. Lucke, E. Köhler, B. Hehmke, M. Schlosser, S. Witt, W. Besch, M. Ziegler: Monoclonal antibody-mediated cytotoxicity against rat Beta cells detected in vitro does not cause Beta-cell destruction in vivo. Diabetologia 35: 608-613 (1992)
21. Ziegler, M., M. Schlosser, J. Hamann, P. Vieregge, F. Lühder, I. Klöting, B. Ziegler: Autoantibodies to glutamate decarboxylase in BB/OK rats. Exp. Clin. Endocrinol. 102: 98-103 (1994)