Efficiency of P-glycoprotein-mediated exclusion of rhodamine dyes from multidrug-resistant cells is determined by their passive transmembrane movement rate

European Journal of Biochemistry

Volumn 248, Issue 1, 1997, Pages 104-112

  Eytan, Gera D ^a   Regev, Ronit ^a   Oren, Galit ^a   Hurwitz, Charles D ^b   Assaraf, Yehuda G ^a  

^a TECHNION ISRAEL INSTITUTE OF TECHNOLOGY   (Israel)

^b Chemotech Technologies Ltd   (Israel)

Author keywords

Anticancer drug; Multidrug resistance; P glycoprotein; Transbilayer transport

Indexed keywords

ADENOSINE TRIPHOSPHATASE; GLYCOPROTEIN P; RHODAMINE; RHODAMINE 123; RHODAMINE 6G; RHODAMINE B;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; DRUG EXCRETION; ENZYME ACTIVITY; FLOW CYTOMETRY; IN VITRO STUDY; MEMBRANE TRANSPORT; MULTIDRUG RESISTANCE; NONHUMAN; PRIORITY JOURNAL; PROTEOLIPOSOME;

ANIMALIA;

EID: 0030785799     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00104.x     Document Type: Article

References (25)

1. Ambudkar, S. V., Lelong, I. H., Zhang, J., Cardarelli, C. O., Gottesman, M. M. & Pastan, I. (1992) Partial purification and reconstitution of the human multidrug-resistance pump: characterization of the drug-stimulatable ATP hydrolysis, Proc. Natl Acad. Sci. USA 89, 8472-8476.
2. Arceci, R. J., Croop, J. M., Horwitz, S. B. & Housman, D. (1988) The gene encoding multidrug resistance is induced and expressed at high levels during pregnancy in the secretory epithelium of the uterus, Proc. Natl Acad. Sci. USA 85, 4350-4354.
3. Assaraf, Y. G., Drori, S., Bachrach, U. & Shaugan-Labay, V. (1994) Determination of multidrug resistance levels in cultured mammalian cells using ornithine decarboxylase activity, Anal. Biochem. 216, 97-109.
4. Borgnia, M. J., Eytan, G. D. & Assaraf, Y. G. (1996) Competition of hydrophobia peptides, cytotoxic drugs, and chemosensitizers on a common P-glycoprotein pharmacophore as revealed by its ATPase activity, J. Biol. Chem. 271, 3163-3171.
5. Bradbury, M. W. (1985) The blood-brain barrier. Transport across the cerebral endothelium, Circ. Res. 57, 213-222.
6. Chabner, B. A. & Fojo, A. (1989) Multidrug resistance: P-glycoprotein and its allies - the elusive foes, J. Natl Cancer Inst. 81, 910-913.
7. Chen, C. J., Chin, J. E., Ueda, K., Clark, D. P., Pastan, I., Gottesman, M. M. & Roninson, I. B. (1986) Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells, Cell 47, 381-389.
8. Cordon-Cardo, C., Jp, O. B., Boccia, J., Casals, D., Bertino, J. R. & Melamed, M. R. (1990) Expression of the multidrug resistance gene product (P-glycoprotein) in human normal and tumor tissues, J. Histochem. Cytochem. 38, 1277-1287.
9. de Wolf, F. A., Maliepaard, M., van Dorsten, F., Berghuis, I., Nicolay, K. & de Kruijff, B. (1990) Comparable interaction of doxorubicin with various acidic phospholipids results in changes of lipid order and dynamics, Biochim. Biophys. Acta 1096, 67-80.
10. de Wolf, F. A., Staffhorst, R. W., Smits, H. P., Onwezen, M. F. & de Kruijff, B. (1993) Role of anionic phospholipids in the interaction of doxorubicin and plasma membrane vesicles: drug binding and structural consequences in bacterial systems, Biochemistry 32, 6688-6695.
11. Drori, S., Eytan, G. D. & Assaraf, Y. G. (1995) Potentiation of anticancer-drug cytotoxicity by multidrug-resistance chemosensitizers involves alterations in membrane fluidity leading to increased membrane permeability, Eur. J. Biochem. 228, 1020-1029.
12. Endicott, J. A. & Ling, V. (1989) The biochemistry of P-glycoprotein-mediated multidrug resistance, Annu. Rev. Biochem. 58, 137-171.
13. Escriba, P. V., Ferrer-Montiel, A. V., Ferragut, J. A. & Gonzalez-Ros, J. M. (1990) Role of membrane lipids in the interaction of daunomycin with plasma membranes from tumor cells : implications in drug-resistance phenomena, Biochemistry 29, 7275-7282.
14. Eytan, G. D., Regev, R., Oren, G. & Assaraf, Y. G. (1996) The role of passive transbilayer drug movement in multidrug resistance and its modulation, J. Biol. Chem. 271, 12897-12902.
15. Lampidis, T. J., Kolonias, D., Podona, T., Israel, M., Safa, A. R., Lothstein, L., Savaraj, N., Tapiero, H. & Priebe, W. (1997) Circumvention of P-GP MDR as a function of anthracycline lipophilicity and charge, Biochemistry 36, 2679-2685.
16. Muszbek, L., Szabo, T. & Fesus, L. (1977) A highly sensitive method for the measurement of ATPase activity, Anal. Biochem. 77, 286-288.
17. Nicolay, K., Timmers, R. J., Spoelstra, E., Van der Neut, R., Fok, J. J., Huigen, Y. M., Verkleij, A. J. & De Kruijff, B. (1984) The interaction of adriamycin with cardiolipin in model and rat liver mitochondrial membranes, Biochim. Biophys. Acta 778, 359-371.
18. O'Connell, A. M., Koeppe, R. E. D. & Andersen, O. S. (1990) Kinetics of gramicidin channel formation in lipid bilayers: transmembrane monomer association, Science 250, 1256-1259.
19. Schinkel, A. H., Smit, J. J., van Tellingen, O., Beijnen, J. H., Wagenaar, E., van Deemter, L., Mol. C. A., van der Valk, M. A., Robanus-Maandag, E. C., te Riele, H. P., Berns, A. J. M. & Borst, P. (1994) Disruption of the mouse mdr1a P-glycoprotein gene leads to a deficiency in the blood-brain barrier and to increased sensitivity to drugs, Cell 77, 491-502.
20. Sharma, R. C., Assaraf, Y. G. & Schimke, R. T. (1991) A phenotype conferring selective resistance to lipophilic antifolates in Chinese hamster ovary cells, Cancer Res. 51, 2949-2959.
21. Sharom, F. J., Yu, X. & Doige, C. A. (1993) Functional reconstitution of drug transport and ATPase activity in proteoliposomes containing partially purified P-glycoprotein, J. Biol. Chem. 268, 24 197-24 202.
22. Stein, W. D. (1990) Channels, carriers, and pumps, Academic Press, Inc., San Diego.
23. Sugawara, I., Ohkochi, E., Hamada, H., Tsuruo, T. & Mori, S. (1988) Cellular and tissue distribution of MRK20 murine monoclonal antibody-defined 85-kDa protein in adriamycin-resistant cancer cell lines, Jpn. J. Cancer Res. 79, 1101-1110.
24. Thiebaut, F., Tsuruo, T., Hamada, H., Gottesman, M. M., Pastan, I. & Willingham, M. C. (1987) Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues, Proc. Natl Acad. Sci. USA 84, 7735-7738.
25. Urbatsch, I. L., al-Shawi, M. K. & Senior, A. E. (1994) Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein, Biochemistry 33, 7069-7076.