Characterization of murine monoclonal antibodies against the Ro52 autoantigen

Clinical and Experimental Immunology

Volumn 110, Issue 1, 1997, Pages 53-62

  Schmitz, M ^a   Bachmann, M ^a   Laubinger J ^a   Thijssen, J P H ^b   Pruijn, G J M ^b  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Autoantigen; Autoimmunity; Monoclonal antibody; Ro SS A; Ro52 antigen

Indexed keywords

AUTOANTIGEN; CELL EXTRACT; EPITOPE; FREUND ADJUVANT; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN; RNA; WHEAT GERM AGGLUTININ;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN ANTIBODY REACTION; ANTIGEN RECOGNITION; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; DELETION MUTANT; FIBROBLAST CULTURE; GENE EXPRESSION; GUINEA PIG; HELA CELL; HEP 2 CELL; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNIZATION; IMMUNOPRECIPITATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; XERODERMA PIGMENTOSUM;

EID: 0030776806     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2249.1997.510-ce1406.x     Document Type: Article

References (31)

1. Tan EM. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 1989; 44:93-151.
2. Harley JB, Alexander E, Bias W et al. Anti-Ro (SS-A) and anti-La (SS-B) in patients with Sjögren's syndrome. Arthritis Rheum 1986; 29:196-206.
3. Van Venrooij WJ, Slobbe RL, Pruijn GJM. Structure and function of La and Ro RNPs. Mol Biol Rep 1993; 18:113-9.
4. Pruijn GJM, Slobbe RL, Van Venrooij WJ. Structure and function of La and Ro RNPs. Mol Biol Rep 1990; 14:43-48.
5. Rader MD, O'Brien CA, Liu YS et al. Heterogeneity of the Ro/SSA antigen. Different molecular forms in lymphocytes and red blood cells. J Clin Invest 1989; 83:1293-8.
6. Itoh Y, Reichlin M. Ro/SS-A antigen in human platelets. Different distributions of the isoforms of Ro/SS-A protein and the Ro/SS-A-binding RNA. Arthritis Rheum 1991; 34:888-93.
7. Ben-Chetrit E, Chan EKL, Sullivan KF, Tan EM. A 52-kD protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med 1988; 167:1560-71.
8. Slobbe RL, Pluk W, Van Venrooij WJ, Pruijn GJM. Ro ribonucleoprotein assembly in vitro. Identification of RNA-protein and protein-protein interactions. J Mol Biol 1992; 227:361-6.
9. Keech CL, Gordon TP, McCluskey J. The immune response to 52 kDa Ro and 60 kDa Ro is linked in experimental autoimmunity. J Immunol 1996; 157:3694-9.
10. Peek R, Pruijn GJM, Van Venrooij WJ. Epitope specificity determines the ability of anti-Ro52 autoantibodies to precipitate Ro ribonucleo-protein particles. J Immunol 1994; 153:4321-9.
11. Itoh Y, Itoh K, Frank MB, Reichlin M. Autoantibodies to the Ro/SSA autoantigen are conformation dependent. II. Antibodies to the denatured form of 52 kD Ro/SSA are a cross reacting subset of antibodies to the native 60 kD Ro/SSA molecule. Autoimmunity 1992; 14:89-95.
12. Kelekar A, Saitta MR, Keene JD. Molecular composition of Ro small ribonucleoprotein complexes in human cells. Intracellular localization of the 60- and 52-kD proteins. J Clin Invest 1994; 93:1637-44.
13. Boire G, Gendron M, Monast N et al. Purification of antigenically intact Ro ribonucleoproteins; biochemical and immunological evidence that the 52-kD protein is not a Ro protein. Clin Exp Immunol 1995; 100:489-98.
14. Ricchiuti V, Pruijn GJM, Thijssen JPH et al. Accessibility of epitopes on the 52-kD Ro/SSA protein (Ro52) and on the Ro RNP associated Ro52 protein as determined by anti-peptide antibodies. J Autoimmun 1997; 10: in press.
15. Peek R, Pruijn GJM, Van der Kemp AJM, Van Venrooij WJ. Subcellular distribution of Ro ribonucleoprotein complexes and their constituents. J Cell Sci 1993; 106:929-35.
16. O' Brien CA, Wolin SL. A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors. Genes Dev 1994; 8:2891-903.
17. Bachmann M, Mayet WJ, Schröder HC et al. Association of La and Ro antigens with intracellular structures in HEp-2 carcinoma cells. Proc Natl Acad Sci USA 1986; 83:7770-4.
18. Bachmann M, Pfeifer K, Schröder HC, Müller WEG. Characterization of the autoantigen La as a nucleic acid dependent ATPase/dATPase with melting properties. Cell 1990; 60:85-93.
19. Bachmann M, Zaubitzer Th, Müller WEG. Presentation and uptake of the nuclear autoantigen La/SSB by mitotic cells. Exp Cell Res 1992; 201:387-96.
20. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680-5.
21. Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 1987; 262:10035-8.
22. Bozic B, Pruijn GJM, Rozman B, Van Venrooij WJ. Refined definition of the 56K and other autoantigens in the 50-60 kDa region. Clin Exp Immunol 1993; 94:227-35.
23. Pruijn GJM, Slobbe RL, Van Venrooij WJ. Analysis of protein - RNA interactions within Ro ribonucleoprotein complexes. Nucleic Acids Res 1991; 19:5173-80.
24. Slobbe RL, Pruijn GJM. Damen WG et al. Detection and occurrence of the 60- and 52-kD Ro (SS-A) antigens and of autoantibodies against these proteins. Clin Exp Immunol 1991; 86:99-105.
25. Keech CL, Gordon TP, McCluskey J. Structural differences between the human and mouse 52-kD Ro autoantigens associated with poorly conserved autoantibody activity across species. Clin Exp Immunol 1996; 104:255-63.
26. Itoh Y, Kriet JD, Reichlin M. Organ distribution of the Ro (SS-A) antigen in the guinea pig. Arthritis Rheum 1990; 33:1815-21.
27. Buyon JP, Slade SG, Reveille JD et al. Autoantibody responses to the 'native' 52-kDa SS-A/Ro protein in neonatal lupus syndromes, systemic lupus erythematosus, and Sjögren's syndrome. J Immunol 1994; 152:3675-84.
28. Ricchiuti V, Briand JP, Meyer O et al. Epitope mapping with synthetic peptides of 52-kD SSA/Ro protein reveals heterogeneous antibody profiles in human autoimmune sera. Clin Exp Immunol 1994; 95:397-407.
29. Chan EKL, Hamel JC, Buyon JP, Tan EM, Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen. J Clin Invest 1991; 87:68-76.
30. Pruijn GJM, Thijssen JPH, Smith PR et al. Anti-La monoclonal antibodies recognizing epitopes within the RNA-binding domain of the La protein show differential capacities to immunoprecipitate RNA-associated La protein. Eur J Biochem 1995; 232:611-9.
31. O'Brien CA, Margelot K, Wolin SL. Xenopus Ro ribonucleoproteins: members of an evolutionarily conserved class of cytoplasmic ribonucleoproteins. Proc Natl Acad Sci USA 1993; 90:7250-4.