Structural differences between the human and mouse 52-kD Ro autoantigens associated with poorly conserved autoantibody activity across species

Clinical and Experimental Immunology

Volumn 104, Issue 2, 1996, Pages 255-263

  Keech, C L ^a   Gordon, T P ^a   McCluskey, J ^a  

^a FLINDERS MEDICAL CENTRE   (Australia)

Author keywords

52 kD Ro; Autoantigens; Ro SS A

Indexed keywords

AUTOANTIBODY; AUTOANTIGEN; COMPLEMENTARY DNA; LEUCINE ZIPPER PROTEIN; RIBONUCLEOPROTEIN; ZINC FINGER PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; AUTOIMMUNITY; CROSS REACTION; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ETIOLOGY; HUMAN; IMMUNOBLOTTING; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0029928578     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.1996.16726.x     Document Type: Article

References (30)

1. Tan EM. Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 1989; 44:93-151.
2. Weng Y-M, McNeilage J, Topfer F, McCluskey J, Gordon T. Identification of a human-specific epitope in a conserved region of the La/SS-B autoantigen. J Clin Invest 1993; 92:1104-8.
3. Harley JB, Rossario MO, Yamagata H, Fox OF, Koren E. Immunologie and structural studies of the lupus/Sjögren's syndrome autoantigen, La/SSB, with a monoclonal antibody. J Clin Invest 1985; 76: 801-6.
4. Slobbe RL, Pruijn GJM, Damen WGM, Van Der Kemp JWCM, Van Venrooij WJ. The detection and occurence of the 60- and 52-kD Ro (SS-A) antigens and of autoantibodies against these proteins. Clin Exp Immunol 1991; 86:99-105.
5. Reichlin M. Molecular definition of the Ro(SS-A) particles: a frequent target of autoimmunity in systemic lupus erythematosus and Sjögren's syndrome. Brit J Rheum 1991; 30 (Suppl. 1):58-62.
6. Wahren M, Skarstein K, Blange I, Pettersson I, Jonsson R. MLR/lpr mice produce anti-Ro 52 000MW antibodies: detection, analysis of specificity and site of production. Immunol 1994; 83:9-15.
7. Visvader J, Begley CG, Adams JM. Differential expression of the LYL, SCL and E24 helix-loop-helix genes within the hemopoictic system. Oncogene 1991; 6:187-94.
8. Itoh K, Itoh Y, Frank MB. Protein heterogeneity in the human Ro/SSA ribonucleoproteins: the 52- and 60-kD Ro/SSA autoantigens are encoded by separate genes. J Clin Invest 1991; 87:177-86.
9. Maniatis T, Sambrook J, Fritsch EF. Molecular cloning: a laboratory manual. Cold Spring Harbor: Cold Spring Harbor Press, 1989.
10. Buckley MF, Gould JW. Preparation of bacteriophage lambda DNA using the TL-100 ultracentrifuge. Anal Biochem 1988; 175:281-3.
11. Marck C. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res 1988; 16:1829.
12. Pearson WR, Lipman DJ. Improved tools for biological sequence analysis. Proc Natl Acad Sci USA 1988; 85:2444.
13. Gordon T, Grove B, Loftus JC, O'Toole T, McMillan R, Lindstrom J, Ginsberg MH. Molecular cloning and preliminary characterization of a novel cytoplasmic antigen recognised by myasthenia gravis sera. J Clin Invest 1992; 90:992-9.
14. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248-5.
15. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bateriophage T4. Nature 1970; 227:680-5.
16. Gordon T, Mavrangelos C, McCluskey J. Restricted epitope recognition by precipitin-negative anti-La/SS-B-positive sera. Arthritis Rheum 1992; 35:663-6.
17. Pullen GR, Fitzgerald MG, Hosking CS. Antibody avidity determination by ELISA using thiocyanate elution. J Immunol Methods 1986; 86:83-87.
18. Chan EKL, Hamel JC, Buyon JP, Tan EM. Molecular definition and sequence motifs of the 52-kD component of human SS-A/Ro autoantigen. J Clin Invest 1991; 87:68-76.
19. Keech CL, Gordon TP, McCluskey J. Cytoplasmic accumulation of the 52 kDa Ro(SS-A) nuclear autoantigen in transfected cell lines. J Autoimmun 1995; 8:699-712.
20. Keech CL, McCluskey J, Gordon TP. Transfection and over-expression of the human 60 kDA Ro/SS-A autoantigen in HEp-2 cells. Clin Immunol Immunopathol 1994; 73:146-51.
21. Itoh Y, Itoh K, Frank MB, Reichlin M. Autoantibodies to the Ro/SSA autoantigen are conformation dependent. II. Antibodies to the denatured form of 52 kD Ro/SSA are a cross reacting subset of antibodies to the native 60 kD Ro/SSA molecule. Autoimmunity 1992; 14:89-95.
22. Ricchiuti V, Briand JP, Meyer O, Isenberg DA, Pruijn G, Muller S. Epitope mapping with synthetic peptides of 52-kD SSA/Ro protein reveals heterogeneous antibody profiles in human autoimmune sera. Clin Exp Immunol 1994; 95:397-407.
23. Frank MB, Itoh K, Fujisaku A, Pontarotti P, Mattei M-G, Neas BR. The mapping of the human 52-kD Ro/SSA autoantigen gene to human chromosome 11, and its polymorphisms. Am J Hum Genet 1993; 52:183-91.
24. Blange I, Ringertz NR, Pettersson I. Identification of antigenic regions of the human 52 kD Ro/SS-A protein recognised by patient sera. J Autoimmun 1994; 7:263-74.
25. Frank MB, Itoh K, McCubbin V. Epitope mapping of the 52-kD Ro/SSA autoantigen. Clin Exp Immunol 1994; 95:390-6.
26. McCauliffe DP, Yin H, Wang L, Lucas L. Autoimmune sera react with multiple epitopes on recombinant 52 and 60 kDa Ro(SSA) proteins. J Rheumatol 1994; 21:1073-80.
27. Kato T, Sasakawa H, Suzuki S, Shirako M, Tashiro F, Nixhioka K, Yamamoto K. Autoepitopes of the 52-kD SS-A/Ro molecule. Arthritis Rheum 1995; 38:990-8.
28. Bozic B, Pruijn GJM, Rozman B, Van Venrooij WJ. Sera from patients with rheumatic diseases recognize different epitope regions on the 52-kD Ro/SS-A protein. Clin Exp Immunol 1993; 94:227-35.
29. Buyon JP, Slade SG, Reville JD, Hamel JC, Chan EKL. Autoantibody responses to the 'native' 52-kDA SS-A/Ro protein in neonatal lupus syndromes, systemic lupus erythematosus, and Sjögren's syndrome. J Immunol 1994; 152:3675-84.
30. Peek R, Pruijn GJM, Van Venrooij WJ. Epitope specificity determines the ability of anti-Ro52 autoantibodies to precipitate Ro RNPs. J Immunol 1994; 153:4321-9.