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6
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1842365055
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note
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s2 strains are described in (4).
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9
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0023662304
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26
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R strain [R. K. Blackman, R. Grinnaila, M. M. D. Koehler, W. M. Gelbart, Cell 49, 497 (1987); (26)]. Genomic DNA was extracted from adult flies [W. McGinnis, A. W. Shermoen, S. K. Beckindorf, Cell 34, 75 (1983)] and Southern blotting was performed (26). RNA was isolated from adult flies by phenol extraction, and Northern analysis was performed with Zetaprobe-gt membranes as described by the manufacturer (Bio-Rad).
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R strain [R. K. Blackman, R. Grinnaila, M. M. D. Koehler, W. M. Gelbart, Cell 49, 497 (1987); (26)]. Genomic DNA was extracted from adult flies [W. McGinnis, A. W. Shermoen, S. K. Beckindorf, Cell 34, 75 (1983)] and Southern blotting was performed (26). RNA was isolated from adult flies by phenol extraction, and Northern analysis was performed with Zetaprobe-gt membranes as described by the manufacturer (Bio-Rad).
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s used the helper plasmid Pπ25.l we. Two additional independent transgenic strains (L1 and L2) each with single inserts showed partial rescue of rover behavior. Mean ± SE (sample size in parentheses) were 7.6 ± 0.43 (30) for L1 and 6.7 ± 0.67 (8) for L2. Rover and sitter controls were 10 ± 0.49 (29) and 5.4 ± 0.34 (33) for L1, and 8.9 ± 0.28 (38) and 4.2 ± 0.26 (25) for L2, respectively.
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We thank J. S. deBelle, J. Ewer, A. J. Hilliker, L. Peypelut, and J. Shore for comments on the manuscript, K. Kaiser and M. Y. Yang for kindly providing the 189Y strain, D. Kalderon for generously sharing the dg2-cDNA and genomic clones, T. M. Lincoln for the generous gift of the rabbit antibody to bovine PKG, and A. Bergmann, M. E. Lane, and C. Nüsslein-Volhard at the Max Planck Institute in Tübingen, Germany, for the use of facilities and assistance in generating transgenic flies. Supported by grants to M.B.S. from the Medical Research Council of Canada, the Natural Sciences and Engineering Research Council of Canada, and the Human Frontiers Science Program. A.R. and R.J.G. were supported in part by grants to New York University from the W. M. Keck Foundation and from A. Utescher.
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