Calcium protects a mesophilic xylanase from proteinase inactivation and thermal unfolding

Journal of Biological Chemistry

Volumn 272, Issue 28, 1997, Pages 17523-17530

  Spurway, Tracey D ^a   Morland, Carl ^a   Cooper, Alan ^b   Sumner, Ian ^c   Hazlewood, Geoffrey P ^d   O'Donnell, Antony G ^a   Pickersgill, Richard W ^c   Gilbert, Harry J ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^d BABRAHAM INSTITUTE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM CHLORIDE; CALCIUM ION; EDETIC ACID; MUTANT PROTEIN; PROTEINASE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BINDING SITE; CALCIUM BINDING; CRYSTAL STRUCTURE; ENZYME INACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PSEUDOMONAS FLUORESCENS;

BETA-GLUCOSIDASE; BINDING SITES; CALCIUM; CATALYSIS; CIRCULAR DICHROISM; CRYSTALLOGRAPHY, X-RAY; ENDO-1,4-BETA XYLANASES; ENDOPEPTIDASES; ENZYME STABILITY; HEAT; KINETICS; MODELS, MOLECULAR; PEPTIDE LIBRARY; PROTEIN CONFORMATION; PROTEIN FOLDING; PSEUDOMONAS FLUORESCENS; SOFTWARE; XYLOSIDASES;

CELLULOMONAS FIMI; CELLVIBRIO JAPONICUS; EQUUS ASINUS; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0030764655     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.28.17523     Document Type: Article

References (31)

1. Biely, P. (1985) Trends Biotechnol. 3, 286-290
2. Gilbert, H. J., and Hazlewood, G. P. (1993) J. Gen. Microbiol. 139, 187-194
3. Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696
4. Henrissat, B., and Bairoch, A. (1993) Biochem. J. 293, 781-788
5. Henrissat, B., Callebaut, I., Fabrega, S., Lehn, P., Mornon, J.-P., and Davies, G. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 7090-7094
6. Jenkins, J., Lo Leggio, L., Harris, G., and Pickersgill, R. (1995) FEBS Lett. 362, 281-285
7. Fontes, C. M. G., Hall, J., Hirst, B. H., Hazlewood, G. P., and Gilbert, H. J. (1995) Appl. Microbiol. Biotechnol. 43, 52-57
8. Keitel, T., Simon, O., Borriss, R., and Heinmann, U. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 5287-5291
9. Chauvaux, S., Souchon, H., Alzari, P. M., Chariot, P., and Beguin, P. (1995) J. Biol. Chem. 270, 9757-9762
10. Welfle, K., Misselwitz, R., Welfle, H., Politz, O., and Borriss, R. (1995) Eur. J. Biochem. 229, 726-735
11. Harris, G. W., Jenkins, J. A., Connerton, I., Cummings, N., Lo Leggio, L., Scott, M., Hazlewood, G. P., Laurie, J. I., Gilbert, H. J., and Pickersgill, R. W. (1994) Structure 2, 1107-1116
12. White, A., Withers, S. G., Gilkes, N. R., and Rose, D. R. (1994) Biochemistry 33, 12546-12552
13. Derewenda, U., Swenson, L., Green, R., Wei, Y., Morosoli, R., Shareck, F., Kluepfel, D., and Derewenda, Z. (1994) J. Biol. Chem. 269, 20811-20814
14. Dominguez, R., Souchon, H., Spinelli, S., Dauter, Z., Wilson, K., Chauvaux, S., Beguin, P., and Alzari, P. M. (1995) Nat. Struct. Biol. 2, 569-576
15. Hall, J., Ali, S., Surani, M. A., Hazlewood, G. P., Clark, A. J., Simmons, J. P., Hirst, B. H., and Gilbert, H. J. (1993) Bio/Technology 11, 376-379
16. Charnock, S. J., Lakey, J. H., Virden, R., Hughes, N., Sinnott, M. L., Hazlewood, G. P., Pickersgill, R., and Gilbert, H. J. (1997) J. Biol. Chem. 272, 2942-2951
17. Hall, J., Hazlewood, G. P., Huskisson, N. S., Durrant, A. J., and Gilbert, H. J. (1989) Mol. Microbiol. 3, 1211-1219
18. Millward-Sadler, S. J., Davidson, K., Hazlewood, G. P., Black, G. W., Gilbert, H. J., and Clarke, J. H. (1995) Biochem. J. 312, 39-48
19. Stoscheck, C. M. (1990) Methods Enzymol. 182, 50-68
20. Sedmak, J. J., and Grossberg, J. R. (1977) Anal. Biochem. 79, 544-552
21. Laemmli, U. K. (1970) Nature 227, 680-685
22. Hames, B. D. (1990) in Gele Electrophoresis of Proteins: A Practical Approach (Hames, B. D., and Rickwood, D., eds) 2nd Ed., pp. 1-139, Oxford University Press, New York
23. Maruyama, K., Mikawa, T., and Ebashi, S. (1984) J. Biochem. 95, 511-519
24. Wiseman, T., Williston, S., Brandis, J. F., and Lin, L. N. (1989) Anal. Biochem. 179, 131-137
25. Cooper, A., and Johnson, C. M. (1994) Methods Mol. Biol. 22, 137-150
26. Cooper, A., and Johnson, C. M. (1994) Methods Mol. Biol. 22, 125-136
27. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680
28. Tull, D., Withers, S. G., Gilkes, N. R., Kilburn, D. G., Warren, R. A. J., and Aebersold, R. (1991) J. Biol. Chem. 266, 15621-15625
29. Seamon, K. B., and Kretsinger, R. H. (1983) in Calcium in Biology (Spiro, T. G., ed) pp. 3-51, John Wiley and Sons, New York
30. Russell, E. W. (1988) in Soil Conditions and Plant Growth (Wild, A., ed) 11th Ed., p. 151, Longman, London
31. Reid, R. E., and Hodges, R. S. (1980) J. Theor. Biol. 84, 401-444