Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against highly polymeric substrates such as xylan

Journal of Biological Chemistry

Volumn 272, Issue 5, 1997, Pages 2942-2951

  Charnock, Simon J ^a   Lakey, Jeremy H ^a   Virden, Richard ^a   Hughes, Neil ^a   Sinnott, Michael L ^b   Hazlewood, Geoffery P ^c   Pickersgill, Richard ^d   Gilbert, Harry J ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c Babraham Institute   (United States)

^d INSTITUTE OF FOOD RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGOSACCHARIDE; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOSE;

ARTICLE; BINDING SITE; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS;

AMINO ACID SEQUENCE; BINDING SITES; ENDO-1,4-BETA XYLANASES; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGODEOXYRIBONUCLEOTIDES; OLIGOSACCHARIDES; POINT MUTATION; PROTEIN CONFORMATION; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; XYLANS; XYLOSIDASES;

ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0031025001     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.272.5.2942     Document Type: Article

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