Site-directed mutations in the third domain of Bacillus thuringiensis δ-Endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles

Applied and Environmental Microbiology

Volumn 62, Issue 1, 1996, Pages 279-282

  Wolfersberger, M G ^a   Chen, X J ^a,c   Dean, D H ^b  

^a Temple University   (United States)

^b OHIO STATE UNIVERSITY   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; BACTERIAL PROTEIN; ENDOTOXIN; MUTANT PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; CELL MEMBRANE PERMEABILITY; INTESTINE BRUSH BORDER; PROTEIN STABILITY; SILKWORM; SITE DIRECTED MUTAGENESIS; SPOROGENESIS;

ANIMALS; ARGININE; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BOMBYX; CELL MEMBRANE PERMEABILITY; ENDOTOXINS; HEMOLYSIN PROTEINS; LARVA; MICROVILLI; MUTAGENESIS, SITE-DIRECTED; MUTATION;

BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); BOMBYX MORI;

EID: 0030022109     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.1.279-282.1996     Document Type: Article

References (23)

1. Carroll, J., and D. J. Ellar. 1993. An analysis of Bacillus thuringiensis δ-endotoxin action on insect-midgut-membrane permeability using a light scattering assay. Eur. J. Biochem. 214:771-778.
2. Chen, X. J., M. K. Lee, and D. H. Dean. 1993. Site-directed mutations in a highly conserved region of Bacillus thuringiensis δ-endotoxin affect inhibition of short circuit current across Bombyx mori midguts. Proc. Natl. Acad. Sci. USA 90:9041-9045.
3. Dean, D. H., and M. J. Adang. 1992. Protein engineering of Bacillus thuringiensis δ-endotoxins and genetic manipulation for plant protection, p. 293-311. In P. R. Shewry and S. Gutteridge (ed.), Plant protein engineering. Cambridge University Press, Cambridge.
4. Ge, A. Z., R. M. Pfister, and D. H. Dean. 1990. Hyperexpression of a Bacillus thuringiensis δ-endotoxin-encoding gene in Escherichia coli: properties of the product. Gene 93:49-54.
5. Ge, A. Z., N. I. Shivarova, and D. H. Dean. 1989. Location of the Bombyx mori specificity domain on a Bacillus thuringiensis δ-endotoxin. Proc. Natl. Acad. Sci. USA 86:4037-4041.
6. Harvey, W. R., and M. G. Wolfersberger. 1979. Mechanism of inhibition of active potassium transport in isolated midgut of Manduca sexta by Bacillus thuringiensis endotoxin. J. Exp. Biol. 83:293-304.
7. Höfte, H., and H. R. Whiteley. 1989. Insecticidal crystal proteins of Bacillus thuringiensis, Microbiol. Rev. 53:242-255.
8. Ihara, H., E. Kuroda, A. Wadano, and M. Himeno. 1993. Specific toxicity of δ-endotoxins from Bacillus thuringiensis to Bombyx mori. Biosci. Biotech. Biochem. 57:200-204.
9. Knowles, B. H., and J. A. T. Dow. 1993. The crystal δ-endotoxin of Bacillus thuringiensis: models for their mechanism of action on the insect gut. Bioessays 15:469-476.
10. Kunkel, T. A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA 82:488-492.
11. Lee, M. K., R. E. Milne, A. Z. Ge, and D. H. Dean. 1991. Location of a Bombyx mori receptor binding region on a Bacillus thuringiensis δ-endotoxin. J. Biol. Chem. 257:3115-3121.
12. Li, J., J. Carroll, and D. J. Ellar. 1991. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 A resolution. Nature (London) 353:815-821.
13. Liebig, B., D. Stetson, and D. H. Dean. 1995. Quantification of the ion channel-forming effect of Bacillus thuringiensis toxins on the midgut of Bombyx mori by voltage clamp. J. Insect. Physiol. 41:17-22.
14. Nishimoto, T., H. Yoshisue, K. Ihara, H. Sakai, and T. Komano. 1994. Functional analysis of block 5, one of the highly conserved amino acid sequences in the 130-kDa cryIVA protein produced by Bacillus thuringiensis subsp. israelensis. FEBS Lett. 348:249-254.
15. Rajamohan, F., E. Alcantara, M. K. Lee, X. J. Chen, A. Curtiss, and D. H. Dean. 1995. Single amino acid changes in domain II of Bacillus thuringiensis CryIAb δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles. J. Bacteriol. 177:2276-2282.
16. Raymond, M. 1985. Présentation d'un programme d'analyse log-probit pour microordinnateur. Cah. O.R.S.T.O.M. Ser. Entomol. Med. Parasitol. 22:117-121.
17. + -gradient-dependent amino acid transport across the brusti border membrane of Pieris brassicae midgut cells. FEBS Lett. 204:213-218.
18. a. Schwartz, J.-L., L. Potvin, X. J. Chen, R. Laprade, and D. H. Dean. Unpublished data.
19. Uemura, T., H. Ihara, A. Wadano, and M. Himeno. 1992. Fluorometric assay of potential change of Bombyx mori midgut brush border membrane induced by δ-endotoxin from Bacillus thuringiensis. Biosci. Biotech. Biochem. 56:1976-1979.
20. Van Rie, J., S. Jansens, H. Höfte, D. Degheele, and H. Van Mellaert. 1989. Specificity of Bacillus thuringiensis δ-endotoxins. Importance of specific receptors on the brush border membrane of the mid-gut of target insects. Eur. J. Biochem. 186:239-247.
21. Wolfersberger, M. G., C. Hofmann, and P. Lüthy. 1986. Interaction of Bacillus thuringiensis delta-endotoxin with membrane vesicles isolated from lepidopteran larval midgut, p. 237-238. In P. Falmagne, J. E. Alouf, F. J. Fehrenbach, J. Jeljaszewicz, and M. Thelestam (ed.). Bacterial protein toxins. Gustav Fischer Verlag, Stuttgart, Germany.
22. Wolfersberger, M. G., P. Lüthy, A. Maurer, P. Parenti, F. V. Sacchi, B. Giordana, and G. M. Hanozet. 1987. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from larval midgut of the cabbage butterfly (Pieris brassicae). Comp. Biochem. Physiol. 86A:301-308.
23. Wolfersberger, M. G., and D. D. Spaeth. 1987. Activity of spore-crystal preparations from twenty serotypes of Bacillus thuringiensis toward Manduca sexta larvae in vivo and in vitro. Z. Angew. Entomol. 103:138-141.