Cytosolic-free calcium increases to greater than 100 micromolar in ATP- depleted proximal tubules

Journal of Clinical Investigation

Volumn 100, Issue 3, 1997, Pages 713-722

  Weinberg, Joel M ^a,c   Davis, Julie A ^a   Venkatachalam, Manjeri A ^b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^c UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

Author keywords

Fura 2FF; Glycine; Hypoxia; Kidney; Mag fura 2

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCIUM ION; GLYCINE;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; HYPOXIA; KIDNEY PROXIMAL TUBULE; MEMBRANE DAMAGE; NONHUMAN; PRIORITY JOURNAL; RABBIT; REOXYGENATION;

EID: 0030755551     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119584     Document Type: Article

References (68)

1. Farber, J.L. 1982. Biology of disease: membrane injury and calcium homeostasis in the pathogenesis of coagulative necrosis. Lab. Invest. 47:114-123.
2. 2+ in cell killing. Chem. Res. Toxicol. 3:484-494.
3. Weinberg, J.M. 1991. The cell biology of ischemic renal injury. Kidney Int. 39:476-500.
4. Weinberg, J.M. 1993. The cellular basis of nephrotoxicity. In Diseases of the Kidney. R.W. Schrier and C.W. Gottschalk, editors. Little, Brown and Co., Boston. 1031-1097.
5. Thomas, C.E., and D.J. Reed. 1989. Current status of calcium in hepatocellular injury. Hepatology. 10:375-384.
6. Weinberg, J.M. 1984. Calcium as a mediator of renal tubule cell injury. Sem. Nephrol. 4:179-191.
7. Herman, B., G.J. Gores, A.-L. Nieminen, T. Kawanishi, A. Harman, and J.J. Lemasters. 1990. Calcium and pH in anoxic and toxic injury. CRC Crit. Rev. Toxicol. 21:127-148.
8. Cheung, J.Y., J.V. Bonventre, C.D. Malis, and A. Leaf. 1986. Calcium and ischemic injury. N. Engl. J. Med. 314:1670-1676.
9. Weinberg, J.M., J.A. Davis, M. Abarzua, and T. Rajan. 1987. Cytoprotective effects of glycine and glutathione against hypoxic injury to renal tubules. J. Clin. Invest. 80:1446-1454.
10. Weinberg, J.M., M.A. Venkatachalam, R. Garza-Quintero, N.F. Roeser, and J.A. Davis. 1990. Structural requirements for protection by small amino acids against hypoxic injury in kidney proximal tubules. FASEB J. 4:3347-3354.
11. Weinberg, J.M., I. Nissim, N.F. Roeser, J.A. Davis, S. Schultz, and I. Nissim. 1991. Relationships between intracellular amino acid levels and protection against injury to isolated proximal tubules. Am. J. Physiol. 260:410-419.
12. Weinberg, J.M., J.A. Davis, N.F. Roeser, and M.A. Venkatachalam. 1991. Role of increased cytosolic free calcium in the pathogenesis of rabbit proximal tubule cell injury and protection by glycine or acidosis. J. Clin. Invest. 87:581-590.
13. Garza-Quintero, R., J.M. Weinberg, J. Ortega-Lopez, J.A. Davis, and M.A. Venkatachalam. 1993. Conservation of structure in ATP depleted proximal tubules. Role of calcium, polyphosphoinositides and glycine. Am. J. Physiol. 265:F605-F623.
14. Weinberg, J.M., J.A. Davis, N.F. Roeser, and M.A. Venkatachalam. 1994. Role of intracellular pH during cytoprotection of proximal tubule cells by glycine or acidosis. J. Am. Soc. Nephrol. 5:1314-1323.
15. Weinberg, J.M., M.A. Venkatachalam, H. Goldberg, N.F. Roeser, and J.A. Davis. 1995. Modulation by Gly, Ca, and acidosis of injury-associated unesterified fatty acid accumulation in proximal tubule cells. Am. J. Physiol. 268: F110-F121.
16. Nurko, S., K. Sogabe, J.A. Davis, N.F. Roeser, M. Defrain, A. Chien, D. Hinshaw, B. Athey, W. Meixner, M.A. Venkatachalam, and J.M. Weinberg. 1996. Contribution of actin cytoskeletal alterations to ATP depletion and calcium-induced proximal tubule cell injury. Am. J. Physiol. 270:F39-F52.
17. Venkatachalam, M.A., and J.M. Weinberg. 1993. Structural effects of intracellular amino acids during ATP depletion. In Surviving Hypoxia. Mechanisms of Control and Adaptation. P.W. Hochachka, P.L. Lutz, T. Sick, M. Rosenthal, and G. van den Thillart, editors. CRC Press, Boca Raton, FL. 474-493.
18. Weinberg, J.M., M.A. Venkatachalam, N.F. Roeser, J.A. Davis, J. Varani, and K.J. Johnson. 1991. Amino acid protection of cultured kidney tubule cells against calcium ionophore-induced lethal cell injury. Lab. Invest. 65:671-678.
19. ++ and glycine on lipid breakdown and death of ATP-depleted MDCK cells. Kidney Int. 48:118-128.
20. Sogabe, K., N.F. Roeser, J.A. Davis, S. Nurko, M.A. Venkatachalam, and J.M. Weinberg. 1996. Calcium dependence of integrity of the actin cytoskeleton of proximal tubule cell microvilli. Am. J. Physiol. 271:F292-F303.
21. Kribben, A., E.D. Wieder, J.F. Wetzels, L. Yu, P.E. Gengaro, T.J. Burke, and R.W. Schrier. 1994. Evidence for role of cytosolic free calcium in hypoxia-induced proximal tubule injury. J. Clin. Invest. 93:1922-1929.
22. Wieder, E.D., M.M. Yaqoob, C.L. Edelstein, T.D. Curren, P.E. Gengaro, R.A. Nemenoff, and R.W. Schrier. 1995. Time course of glycine cytoprotection in hypoxic proximal tubules. Proc. XIIIth Int. Congr. Nephrol. 166(Abstr.)
23. 2+ and injury in anoxic rabbit proximal tubules. Kidney Int. 46:223-229.
24. 2+]i and cell injury in rabbit renal proximal tubules. J. Am. Soc. Nephrol. 5:892(Abstr.)
25. Weinberg, J.M., J.A. Davis, M. Abarzua, and T. Kiani. 1990. Glycine-dependent protection of proximal tubules against lethal cell injury due to inhibitors of mitochondrial ATP production. Am. J. Physiol. 258:C1127-C1140.
26. Cobbold, P.H., and T.J. Rink. 1987. Fluorescence and bioluminescence measurement of cytoplasmic free calcium. Biochem. J. 248:313-328.
27. Raju, B., E. Murphy, L.A. Levy, R.D. Hall, and R.E. London. 1989. A fluorescent indicator for measuring cytosolic free magnesium. Am. J. Physiol. 256:C540-C548.
28. Konishi, M., S. Hollingworth, A.B. Harkins, and S.M. Baylor. 1991. Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra. J. Gen. Physiol. 97:271-301.
29. Murphy, E. 1993. Measurement of intracellular ionized magnesium. Miner. Electrolyte Metab. 19:250-258.
30. 2+ using mag-fura-2. Am. J. Physiol. 263:C300-C307.
31. Konishi, M., and J.R. Berlin. 1993. Ca transients in cardiac myocytes measured with a low affinity fluorescent indicator, furaptra. Biophys. J. 64: 1331-1343.
32. 2+ concentration in Limulus ventral photoreceptors using fluorescent dyes. J. Gen. Physiol. 105:95-116.
33. 2+. Am. J. Physiol. 264:C1259-C1269.
34. Harman, A.W., A.-L. Nieminen, J.J. Lemasters, and B. Herman. 1990. Cytosolic free magnesium, ATP, and blebbing during chemical hypoxia in cultured rat hepatocytes. Biochem. Biophys. Res. Commun. 170:477-483.
35. 2+ concentrations following metabolic inhibition in opossum kidney cells. Biochim. Biophys. Acta. 1181:307-315.
36. 2+ levels. J. Lab. Clin. Med. 122:260-272.
37. 2 deprivation injury. J. Am. Soc. Nephrol. 1:837-840.
38. Garza-Quintero, R., J. Ortega-Lopez, J.H. Stein, and M.A. Venkatachalam. 1990. Alanine protects rabbit proximal tubules against anoxic injury in vitro. Am. J. Physiol. 258:F1075-F1083.
39. Weinberg, J.M., J.A. Davis, A. Lawton, and M. Abarzua. 1988. Modulation of cell nucleotide levels of isolated kidney tubules. Am. J. Physiol. 254: F311-F322.
40. Weinberg, J.M., J.A. Davis, M. Abarzua, and T. Kiani. 1989. Relationship between cell ATP and glutathione content and protection by glycine against hypoxic proximal tubule cell injury. J. Lab. Clin. Med. 113:612-623.
41. Heytler, P.G. 1979. Uncouplers of oxidative phosphorylation. Methods. Enzymol. 55:462-472.
42. Takano, T., S.P. Soltoff, S. Murdaugh, and L.J. Mandel. 1985. Intracellular respiratory dysfunction and cell injury in short-term anoxia of rabbit renal proximal tubules. J. Clin. Invest. 76:2377-2384.
43. Becker, G.L., G. Fiskum, and A.L. Lehninger. 1980. Regulation of free calcium by liver mitochondria and endoplasmic reticulum. J. Biol. Chem. 255: 9009-9012.
44. Nicchitta, C.V., and J.R. Williamson. 1984. Spermine. A regulator of mitochondrial calcium cycling. J. Biol. Chem. 259:12978-12983.
45. 2+ fluxes. Annu. Rev. Physiol. 51:107-124.
46. Carafoli, E. 1987. Intracellular calcium homeostasis. Annu. Rev. Biochem. 56:395-433.
47. Somlyo, A.P. 1985. Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo. Nature (Lond.). 314:622-625.
48. Carafoli, E. 1991. Calcium pump of the plasma membrane. Physiol. Rev. 71:129-153.
49. Jacobs, W.R., M. Sgambati, G. Gomez, P. Vilaro, M. Higdon, P.D. Bell, and L.J. Mandel. 1991. Role of cytosolic Ca in renal tubule damage induced by anoxia. Am. J. Physiol. (Cell Physiol.). 260:C545-C554.
50. LeFurgey, A., A.J. Spencer, W.R. Jacobs, P. Ingram, and L.J. Mandel. 1991. Elemental microanalysis of organelles in proximal tubules. I. Alterations in transport and metabolism. J. Am. Soc. Nephrol. 1:1305-1320.
51. Spencer, A.J., A. LeFurgey, P. Ingram, and L.J. Mandel. 1991. Elemental microanalysis of organelles in proximal tubules. II. Effects of oxygen deprivation. J. Am. Soc. Nephrol. 1:1321-1333.
52. Beck, F.X., A. Ohno, A. Dorge, and K. Thurau. 1995. Ischemia-induced changes in cell element composition and osmolyte contents of outer medulla. Kidney Int. 48:449-457.
53. 2+ efflux mechanisms in proximal tubule cells. J. Am. Soc. Nephrol. 7:1809(Abstr.)
54. Snowdowne, K.W., C.C. Freudenrich, and A.B. Borle. 1985. The effects of anoxia on cytosolic free calcium, calcium fluxes, and cellular ATP levels in cultured kidney cells. J. Biol. Chem. 260:11619-11626.
55. 2+] in single proximal tubule cell (PTC) during anoxia and reoxygenation. Fed. Proc. 46:1327(Abstr.)
56. McCoy, C.E., A.M. Selvaggio, E.A. Alexander, and J.H. Schwartz. 1988. Adenosine triphosphate depletion induces a rise in cytosolic free calcium in canine renal epithelial cells. J. Clin. Invest. 82:1326-1332.
57. Phelps, P.C., M.W. Smith, and B.F. Trump. 1989. Cytosolic ionized calcium and bleb formation after acute cell injury of cultured rabbit renal tubule cells. Lab. Invest. 60:630-642.
58. Greene, E.L., and M.S. Paller. 1994. Calcium and free radicals in hypoxia/reoxygenation injury of renal epithelial cells. Am. J. Physiol. 266:F13-F20.
59. Duran, M.-A., D. Spencer, M. Weise, N.O. Kronfol, R.F. Spencer, and D.E. Oken. 1990. Renal epithelial amino acid concentrations in mercury-induced and postischemic acute renal failure. Toxicol. Appl. Pharmacol. 105:183-194.
60. Hofer, A.M., and T.E. Machen. 1994. Direct measurement of free Ca in organelles of gastric epithelial cells. Am. J. Physiol. 267:G442-G451.
61. 2+ stores in sarcoplasmic and endoplasmic reticulum. Science (Wash. DC). 275:1643-1648.
62. Lew, P.D., A. Monod, F.A. Waldvogel, B. Dewald, M. Baggiolini, and T. Pozzan. 1986. Quantitative analysis of the cytosolic free calcium dependency of exocytosis from three subcellular compartments in intact human neutrophils. J. Cell Biol. 102:2197-2204.
63. Williams, D.A., and F.S. Fay. 1990. Intracellular calibration of the fluorescent calcium indicator Fura-2. Cell Calcium. 11:75-83.
64. Davis, J.A., and J.M. Weinberg. 1988. Effects of glycine and GSH on toxic maneuvers altering tubule cell plasma membrane cation permeability. Clin. Res. 36:517A(Abstr.)
65. Nakamura, H., R.A. Nemenoff, J.H. Gronich, and J.V. Bonventre. 1991. Subcellular characteristics of phospholipase A2 activity in the rat kidney. Enhanced cytosolic, mitochondrial, and microsomal phospholipase A2 enzymatic activity after renal ischemia and reperfusion. J. Clin. Invest. 87:1810-1818.
66. Bjornstad, P. 1966. Phospholipase activity in rat liver mitochondria studied by the use of endogenous substrates. J. Lipid Res. 7:612-629.
67. Weinberg, J.M., J.A. Davis, and B. Trivedi. 1988. Calcium comparmentation in isolated renal tubules in suspension. Biochem. Med. Metab. Biol. 39: 234-245.
68. Dong, Z., P. Saikumar, M. Venkatachalam, and J. Weinberg. 1996. Intracellular calcium levels that determine the survival of glycine protected ATP depleted cells. J. Am. Soc. Nephrol. 7:1823(Abstr.)