Methylene blue is a muscarinic antagonist in cardiac myocytes

Molecular Pharmacology

Volumn 52, Issue 3, 1997, Pages 482-490

  Abi Gerges, Najah ^a   Eschenhagen, Thomas ^a,b   Hove Madsen, Leif ^a,c   Fischmeister, Rodolphe ^a   Mery, Pierre François ^a,d  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^d INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE; CHLORPROMAZINE; GUANYLATE CYCLASE INHIBITOR; ISOPRENALINE; METHYLENE BLUE; MUSCARINIC M2 RECEPTOR ANTAGONIST; MUSCARINIC RECEPTOR; PIPERAZINE DERIVATIVE; QUINUCLIDINYL BENZILATE;

ADOLESCENT; ANIMAL CELL; ARTICLE; BINDING KINETICS; CALCIUM CURRENT; DRUG EFFECT; DRUG MECHANISM; DRUG RECEPTOR BINDING; HEART MUSCLE CELL; LIGAND BINDING; MALE; NONHUMAN; PATCH CLAMP; POTASSIUM CURRENT; PRIORITY JOURNAL; RAT;

EID: 0030750801     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.52.3.482     Document Type: Article

References (39)

1. Ignarro, L. J., T. M. Burke, K. S. Wood, M. S. Wolin, and P. J. Kadowitz. Association between cyclic GMP accumulation and acetylcholine-elicited relaxation of bovine intrapulmonary artery. J. Pharmacol. Exp. Ther. 228:682-690 (1984).
2. Martin, W., G. M Villani, D. Jothianandan, and R. F. Furchgott. Selective blockade of endothelium-dependent and glyceryl trinitrate-induced relaxation by hemoglobin and methylene blue in the rabbit aorta. J. Pharmacol. Exp. Ther. 232:708-716 (1985).
3. Ignarro, L. J., R. G. Harbison, K. S. Wood, and P. J. Kadowitz. Dissimilarities between methylene blue and cyanide on relaxation and cyclic GMP formation in endothelium-intact intrapulmonary artery caused by nitrogen oxide-containing vasodilators and acetylcholine. J. Pharmacol. Exp. Ther. 236:30-36 (1986).
4. Tremblay, J., R. G. Harbison, and P. Hamet. Cyclic GMP in cell function. Adv. Second Messenger Phosphoprotein Res. 22:320-383 (1988).
5. Moncada, S., R. M. Palmer, and E. A. Higgs. Nitric oxide: physiology, pathophysiology and pharmacology. Pharmacol. Rev. 43:109-142 (1991).
6. Rubanyi, G. M., and P. M. Vanhoutte. Superoxide anions and hyperoxia inactivate endothelium-derived relaxing factor. Am. J. Physiol. 250:H822-H827 (1986).
7. Vanhoutte P. M. Other endothelium-derived vasoactive factors. Circulation 87:V-9-V-17 (1993).
8. Cook, R. P. The antagonism of acetyl choline by methylene blue. J. Physiol.(Lond.) 62:160-165 (1926).
9. Kelly, R. A., J.-L. Balligand, and T. W Smith. Nitric oxide and cardiac function. Circ. Res. 79:363-380 (1996).
10. Méry, P.-F., N. Abi-Gerges, G. Vandecasteele, J. Jurevicius, T. Eschenhagen, and R. Fischmeister. Muscarinic regulation of the L-type calcium current in isolated cardiac myocytes. Life Sci. 60:1113-1120 (1997).
11. ++-dependent constitutive nitric oxide synthase is not involved in the cyclic GMP-increasing effects of carbachol in ventricular cardiomyocytes. J. Pharmacol. Exp. Ther. 266:919-925 (1993).
12. Ca by carbachol and sodium nitroprusside. Pflueg. Arch. Eur. J. Physiol. 426:419-426 (1994).
13. Balligand, J.-L., L. Kobzik, X. Q. Han, D. M. Kaye, L. Belhassen, D. S. Ohara, R. A. Kelly, T. W. Smith, and T. Michel. Nitric oxide-dependent parasympathetic signaling is due to activation of constitutive endothelial (type III) nitric oxide synthase in cardiac myocytes. J. Biol. Chem. 270: 14582-14586 (1995).
14. Han, X., Y. Shimoni, and W. R. Giles. A cellular mechanism for nitric oxide-mediated cholinergic control of mammalian heart rate. J. Gen. Physiol. 106:45-65 (1995).
15. 2+ current in adult rabbit atrioventricular nodal cells. Circ. Res. 78:998-1008 (1996).
16. Zakharov, S. I., S. Piermici, G. K. Kumar, N. R. Prabhakar, and R. D. Harvey. Nitric oxide synthase activity in guinea pig ventricular myocytes is not involved in muscarinic inhibition of cAMP-regulated ion channels. Circ. Res. 78:925-935 (1996).
17. Brady, A. J. B., J. B. Warren, P. A. Poole-Wilson, T. J. Williams, and S. E. Harding. Nitric oxide attenuates cardiac myocyte contraction. Am. J. Physiol. 265:H176-H182 (1993).
18. Mohan, P., D. L. Brutsaert, W. J. Paulus, and S. U. Sys. Myocardial contractile response to nitric oxide and cGMP. Circulation 93:1223-1229 (1996).
19. 2+ current: involvement of cGMP-inhibited and cGMP-stimulated phosphodiesterases through guanylyl cyclase activation. J. Biol. Chem. 268:26286-26295 (1993).
20. Balligand, J.-L., R. A. Kelly, P. A. A Marsden, T. W. Smith, and T. Michel. Control of cardiac muscle cell function by an endogenous nitric oxide signaling system. Proc. Natl. Acad. Sci. USA 90:347-351 (1993).
21. + currents in frog cardiac myocytes. Br. J. Pharmacol. 121:1369-1377 (1997).
22. Marshall, J. J., E. P. Wei, and H. A. Kontos. Independent blockade of cerebral vasodilation from acetylcholine and nitric oxide. Am. J. Physiol. 255:H847-H854 (1988).
23. Wolin, M. S., P. D. Cherry, J. M. Rodenburg, E. J. Messina, and G. Kaley. Methylene blue inhibits vasodilation of skeletal muscle arterioles to acetylcholine and nitric oxide via the extracellular generation of superoxide anion. J. Pharmacol. Exp. Ther. 254:872-876 (1990).
24. Mayer, B., F. Brunner, and K. Schmidt. Inhibition of nitric oxide synthesis by methylene blue. Biochem. Pharmacol. 45:367-374 (1993).
25. Palacios, M., J. Padron, L. Glaria, A. Rojas, R. Delgado, R. Knowles, and S. Moncada. Chlorpromazine inhibits both the constitutive nitric oxide synthase and the induction of nitric oxide synthase after LPS challenge. Biochem. Biophys. Res. Commun. 196:280-285 (1993).
26. Tseng, G.-N. Cell swelling increases membrane conductance of canine cardiac cells: evidence for a volume-sensitive Cl channel. Am. J. Physiol. 262:C1056-C1068 (1992).
27. + channels by chlorpromazine in rat ventricular myocytes. J. Pharmacol. Exp. Ther. 271:632-637 (1994).
28. Mironov, S. L., and H. D. Lux. Cytoplasmic alkalinization increases high-threshold calcium current in chick dorsal root ganglion neurones. Pflueg. Arch. Eur. J. Physiol. 419:138-143 (1991).
29. Abi-Gerges, N., T. Eschenhagen, L. Hove-Madsen, P.-F. Méry, and R. Fischmeister. Methylene blue is a muscarinic antagonist in rat cardiac myocytes (abstract). Biophys J. 72:A34 (1997).
30. Scamps, F., E. Mayoux, D. Charlemagne, and G. Vassort. Calcium current in single cells isolated from normal and hypertrophied rat heart. Circ. Res. 67:199-208 (1990).
31. + currents in frog cardiac myocytes. J. Gen. Physiol. 104:941-959 (1994).
32. Fischmeister, R., and H. C. Hartzell. Mechanisms of action of acetylcholine on calcium current in single cells from frog ventricle. J. Physiol. (Lond.) 376:183-202 (1986).
33. Bradford, M. M. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248-254 (1976).
34. Nedoma, J., J. Slavikova, and S. Tucek. Muscarinic acetylcholine receptors in the heart of rats before and after birth. Pflueg. Arch. Eur. J. Physiol. 406:45-50 (1986).
35. McMahon, K. Developmental changes of the G proteins-muscarinic cholinergic receptor interactions in rat heart. J. Pharmacol. Exp. Ther. 251: 372-377 (1989).
36. Sterin-Borda, L., A. V. Echague, C. P. Leiros, A. Genaro, and E. Borda. Endogenous nitric oxide signalling system and the cardiac muscarinic acetylcholine receptor-inotropic response. Br. J. Pharmacol. 115:1525-1531 (1995).
37. + currents in frog and rat cardiac cells. J. Physiol. (Lond.) 461:743-765 (1993).
38. Mubagwa, K., T. Shirayama, M. Moreau, and A. J. Pappano. Effects of PDE inhibitors and carbachol on the L-type Ca current in guinea-pig ventricular myocytes. Am. J. Physiol. 264:H1353-H1363 (1993).
39. 2+ current in rabbit atrioventricular node cells. Am. J. Physiol. 271:H2274-H2282 (1996).