Potential misconceptions in dopamine transporter assays arising from the binding of [125I]RTI-121 to filters: Effect of ions and cocaine

Journal of Neuroscience Methods

Volumn 75, Issue 2, 1997, Pages 179-186

  Chen, Nian Hang ^a   Ding, Jian Hua ^a   Wang, You Lin ^a   Reith, Maarten E A ^b  

^a NANJING MEDICAL UNIVERSITY   (China)

^b UNIVERSITY OF ILLINOIS   (United States)

Author keywords

Dopamine transporter; Filter binding; Ion dependency; Rat striatum; 125I RTI 121

Indexed keywords

1 [1 (2 BENZO[B]THIENYL)CYCLOHEXYL]PIPERIDINE; 1 [2 (BENZHYDRYLOXY)ETHYL] 4 (3 PHENYLPROPYL)PIPERAZINE; 3BETA (4 IODOPHENYL) 2BETA TROPANECARBOXYLIC ACID ISOPROPYL ESTER; COCAINE; DOPAMINE; IODINE 125; MAZINDOL; POTASSIUM ION; SODIUM ION;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; BRAIN SYNAPTOSOME; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORPUS STRIATUM; DOPAMINERGIC SYSTEM; ION TRANSPORT; ISOTOPE LABELING; MALE; MEMBRANE BINDING; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0030750109     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)00070-8     Document Type: Article

References (23)

1. + dependence of the neuronal transport of dopamine. J Neurochem 1992;59:1795-804.
2. 125I]iodophenyl)tropane-2β-carboxylic acid isopropyl ester. Mol Pharmacol 1995;47:779-86.
3. Carroll FI, Kotian P, Dehghani A, Gray JL, Kuzemko MA, Parham KA, Abraham P, Lewin AH, Boja JW, Kuhar MJ. Cocaine and 3β-(4′-substituted phenyl)tropane-2β-carboxylic acid ester and amide analogues. New high-affinity and selective compounds for the dopamine transporter. J Med Chem 1995;38:379-88.
4. 125I] 3β-(4-iodophenyl)tropane-2β-carboxylic acid isopropyl ester to the dopamine transporter at a physiologically relevant temperature: mutually exclusive binding and different ionic requirement for various uptake blockers and substrates. Synapse 1997a;25:155-62.
5. 125I]RTI-121 binding to the dopamine transporter under physiological condition. Acta Pharmacol Sin 1997;18:115-20.
6. 125I]iodophenyl)tropan-2β-carboxylic acid isopropyl ester to the dopamine transporter. J Neurochem 1997, in press.
7. 3H]WIN 35,428 binding to the dopamine uptake carrier: I. Effect of tonicity and buffer composition. J Neurosci Methods 1994;51:23-30.
8. Izenwasser S, Rosenberger JG, Cox BM. The cocaine analog WIN 35428 binds to two sites in fresh rat caudate-putamen: significance of assay procedures. Life Sci 1993;52:141-5.
9. De Lean A, Munson PJ, Rodbard D. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay and physiological dose-response curves. Am J Physiol 1978;235:E97-E102.
10. 3H]GBR12935 to rat and bovine striata. Membr Biochem 1993;10:129-44.
11. Kirifides AL, Harvey JA, Aloyo VJ. The low affinity binding site for the cocaine analog, WIN 35 428 is an artifact of freezing caudate tissue. Life Sci 1992;50:139-42.
12. 125I]RTI-121 binding sites in human striatum and medial temporal lobe. J Pharmacol Exp Ther 1995;274:1473-83.
13. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
14. 3H]2β-carbomethoxy-3β-(4-fluorophenyl)tropane. Mol Pharmacol 1989;36:518-24.
15. McElvain JS, Schenk JO. A multisubstrate mechanism of striatal dopamine uptake and its inhibition by cocaine. Biochem Pharmacol 1992;43:2189-99.
16. Munson PJ, Rodbard D. A versatile computerized approach for characterization of ligand-binding systems. Anal Biochem 1988;107:220-39.
17. 3H]cocaine binding sites. Naunyn-Schmied Arch Pharmacol 1995;351:136-45.
18. 3H]tropane to the dopamine uptake carrier. J Neurochem 1993;61:167-77.
19. 3H]WIN 35,428 binding to the dopamine uptake carrier: II. Effect of membrane fractionation procedure and freezing. J Neurosci Methods 1994;51:31-8.
20. Scheffel U, Dannals RF, Wong DF, Yokoi F, Carroll FI, Kuhar MJ. Dopamine transporter imaging with novel, selective cocaine analogs. Neuroreport 1992;3:969-72.
21. 125I]RTI-121. Synapse 1995;21:364-72.
22. Xu C, Coffey LL, Reith MEA. Translocation of dopamine and binding of 2β-carbomethoxy-3β-(4-fluoropheny)tropane (WIN 35,428) measured under identical conditions in rat striatal synaptosomal preparations. Inhibiton by various blockers. Biochem Pharmacol 1995;49:339-50.
23. Zimanyi I, Lajtha A, Reith MEA. Comparison of characteristics of dopamine uptake and mazindol binding in mouse striatum. Naunyn-Schmied Arch Pharmacol 1989;340:626-32.