Preparation of the antibodies against recombinant human thymidylate synthase for the detection of its intratumoral levels and the application to sensitivity-study of 5-fluorouracil

Oncology Reports

Volumn 4, Issue 4, 1997, Pages 685-690

  Okabe, Hiroyuki ^a   Tsujimoto, Hiroaki ^a   Fukushima, Masakazu ^a  

^a TAIHO PHARMACEUTICAL CO LTD   (Japan)

Author keywords

5 fluorouracil; Polyclonal antibody; Recombinant protein; Thymidylate synthase; Xenografts

Indexed keywords

FLUOROURACIL; POLYCLONAL ANTIBODY; THYMIDYLATE SYNTHASE;

ANTIGEN ANTIBODY REACTION; ANTIGEN EXPRESSION; ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; HUMAN CELL; HUMAN TISSUE; IMMUNE RESPONSE; IMMUNOBLOTTING; IMMUNOCHEMISTRY; PREDICTION; PRIORITY JOURNAL; PROGNOSIS; PROTEIN ANALYSIS; RETROSPECTIVE STUDY;

EID: 0030743239     PISSN: 1021335X     EISSN: None     Source Type: Journal    
DOI: 10.3892/or.4.4.685     Document Type: Article

References (24)

1. Hartman KY and Heidelberger C: Studies on fluorinated pyrimidines, XIII. Inhibition of thymidylate synthase. J Biol Chem 236: 3006-3018, 1961.
2. Langenback RJ, Darnenberg PV and Heidelberger C: Thymidylate synthase: mechanism of inhibition by 5-fluoro-2′-deoxyuridylate. Biochem Biophys Res Commun 48: 1565-1571, 1972.
3. Santi DV and McHenry CS: 5-Fluoro-2′-deoxyuridylate: covalent complex with thymidylate synthase. Proc Natl Acad Sci USA 69: 1855-1857, 1972.
4. Spears CD, Shahinian AH, Moran RG, Heidelberger C and Corbett TH: In vivo kinetics of thymidylate synthase inhibition in 5-fluorouracil-sensitive and-resistant murine colon adenocarcinoma. Cancer Res 43: 450-456, 1982.
5. Spears CP, Gustavsson BG, Berne M, Frosing R, Bernstein L and Hayes AA: Mechanism of innate resistance to thymidylate synthase inhibition after 5-fluorouracil. Cancer Res 48: 5894-5900, 1988.
6. Johnston PG, Fisher ER, Rockette HE, Fisher B, Welmark N, Drake JC, Chabner BA and Allegra CJ: The role of thymidylate synthase expression in prognosis and outcome of adjuvant chemotherapy in patients with rectal cancer. J Clin Oncol 12: 2640-2647, 1994.
7. Peters GJ, van der Wolt CL, van Groeningen CJ, Smid K, Meijer S and Pinedo HM: Thymidylate synthase inhibition after administration of fluorouracil with or without Leucovorin in colon cancer patients: implications for treatment with fluorouracil. J Clin Oncol 12: 2035-2042, 1994.
8. Johnston PG, Lenz H-J, Leichman CG, Danenberg KD, Allegra CJ, Danenberg PV and Leichman L: Thymidylate synthase gene and protein expression correlate and are associated with response to 5-fluorouracil in human colorectal and gastric tumors. Cancer Res 55: 1407-1412, 1995.
9. Peters GJ, van der Wilt CL, van Triest B, Codacci-Pisanelli G, Johnston PG, van Groeningen CJ and Pinedo HM: Thymidylate synthase and drug resistance. Eur J Cancer 31A: 1299-1305, 1995.
10. Leichman L, Lenz H-J, Leichman CG, Groshen LS, Danenberg GK, Baranda J, Spears CP, Boswell W, Silberman H, Ortega A, Stain S, Beart R and Danenberg P: Quantitation of intratumoral thymidylate synthase expression predicts for resistance to protracted infusion of 5-fluorouracil and weekly Leucovorin in disseminated colorectal cancers: preliminary report from an ongoing trial. Eur J Cancer 31A: 1306-1310, 1995.
11. Lenz H-J, Leichman CG, Danenberg KD, Danenberg PV, Groshen S, Cohen H, Laine L, Crookes P, Silberman H, Baranda J, Gracia Y, Li J and Leichman L: Thymidylate synthase mRNA level in adenocarcinoma of the stomach: A predictor for primary tumor response and overall survival. J Clin Oncol 4: 176-182, 1996.
12. Johnston P, Liang C, Henry S, Chabner B and Allegra C: Production and characterization of monoclonal antibodies that localize human thymidylate synthase in the cytoplasm of human cells and tissues. Cancer Res 51: 6668-6676, 1991.
13. Jastreboff MM, Todd MB and Malech HL: Isolation and functional effect of monoclonal antibodies binding to thymidylate synthase. Biochemistry 24: 587-592, 1985.
14. Aherne GW, Hardcastle A and Newton R: Measurement of human thymidylate synthase (hTS) in cell lines using ELISA. Ann Oncol 3 (Suppl 1): 77, 1992.
15. Takeishi K, Kaneda S, Ayusawa D, Shimizu K, Gotoh O, Seno T: Nucleotide sequence of a functional cDNA for human thymidylate synthase. Nucleic Acid Res 13: 2035-2043, 1985.
16. Spears CP, Shahinian AH and Moran RG: In vivo kinetics of thymidylate synthase inhibition in 5-fluorouracil-sensitive and-resistant murine colon adenocarcinomas. Cancer Res 42: 450-456, 1982.
17. Carmichael J, DeGraff WG, Gazdar AF, Minna JD and Mitchell JB: Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47: 936-942, 1987.
18. Lesuffleur T, Kornowski A, Luccioni C, Muleris M, Barbat A, Beaumatin J, Dussaulx E, Dutrillaux B and Zweibaum A: Adaptation to 5-fluorouracil of the heterogenous human colon tumor cell line HT-29 results in the selection of cells committed to differentiation. Int J Cancer 49: 721-730, 1991.
19. Fujii S, Ikenaka K, Fukushima M and Shirasaka T: Effect of uracil and its derivatives on antitumor activity of 5-fluorouracil and 1-(2-tetrahydrofuryl)-5-fluorouracil. Gann 69: 763-772, 1978.
20. Kimura K, Suga S, Shimaji T, Kitamura M, Kubo K, Suzuoki Y and Isobe K: Clinical basis of chemotherapy for gastric cancer with uracil and 1-(2-tetrahydrofuyl)-5-fluorouracil. Jpn Soc Gastroenterol 15: 324-329, 1980.
21. Berger SH, Jenh C-H, Johnston LF and Berger FG: Thymidylate synthase overproduction and gene amplification in fluorodeoxyuridine-resistant human cells. Mol Pharmacol 28: 461-467, 1985.
22. Achele C, Sobrero A, Faderan MA and Bertino JR: Novel mechanism(s) of resistance to 5-fluorouracil in human colon cancer (HCT-8) sublines following exposure to two different clinically relevant dose schedules. Cancer Res 52: 1855-1864, 1992.
23. Johnston PG, Drake JC, Trepel J and Allegra CJ: Immunological quantitation of thymidylate synthase using the monoclonal antibody TS106 in 5-fluorouracil-sensitive and-resistant human cancer cell lines. Cancer Res 52: 4306-4312, 1992.
24. Copur S, Aiba K, Drake JC, Allegra CJ and Chu E: Thymidylate synthase gene amplification in human colon cancer cell lines resistant to 5-fluorouracil. Biochem Pharmacol 49: 1419-1426, 1995.