Cloning and sequencing of a novel gene (recG) that affects the quinolone susceptibility of staphylococcus aureus

Antimicrobial Agents and Chemotherapy

Volumn 41, Issue 8, 1997, Pages 1770-1774

  Niga, Toshiyuki ^a   Yoshida, Hiroaki ^a   Hattori, Hiroaki ^a   Nakamura, Shinichi ^a   Ito, Hideaki ^a  

^a DAINIPPON SUMITOMO PHARMA CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; AMPICILLIN; CHLORAMPHENICOL; CIPROFLOXACIN; DNA; ERYTHROMYCIN; GENE PRODUCT; HELICASE; KANAMYCIN; MITOMYCIN; NORFLOXACIN; NUCLEOTIDE; OXOLINIC ACID; POLYPEPTIDE; QUINOLONE; SPARFLOXACIN; TETRACYCLINE;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM; DNA REPAIR; GENE; GENE SEQUENCE; HYBRIDIZATION; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS;

EID: 0030741937     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.41.8.1770     Document Type: Article

References (35)

1. Fasching, C. E., F. C. Tenover, T. G. Slama, L. M. Fisher, S. Sreedharan, M. Oram, K. Willard, L. M. Sinn, D. N. Gerding, and L. R. Peterson. 1991. gyrA mutations in ciprofloxacin-resistant, methicillin-resistant Staphylococcus aurens from Indiana, Minnesota, and Tennessee. J. Infect. Dis. 164:976-979.
2. Ferrero, L., B. Cameron, B. Manse, D. Lagneaux, J. Crouzet, A. Famechon, and F. Blanche. 1994. Cloning and primary structure of Staphylococcus aureus DNA topoisomerase IV: a primary target of fluoroquinolones. Mol. Microbiol. 13:641-653.
3. Ferrero, L., B. Cameron, and J. Crouzet. 1995. Analysis of gyrA and grlA mutations in stepwise-selected ciprofloxacin-resistant mutants of Staphylococcus aureus. Antimicrob. Agents Chemother. 39:1554-1558.
4. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J.-F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L.-I. Liu, A. Glodek, J. M. Kelley, J. F. Weidman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehm, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
5. Gorbalenya, A. E., E. V. Koonin, A. P. Donchenko, and V. M. Blinov. 1989. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res. 17:4713-4730.
6. Goswitz, J. J., K. E. Willard, C. E. Fasching, and L. R. Peterson. 1992. Detection of gyrA gene mutations associated with ciprotloxacin resistance in methicillin-resistant Staphylococcus aureus: analysis by polymerase chain reaction and automated direct DNA sequencing. Antimicrob. Agents Chemother. 36:1166-1169.
7. Hiasa, H., D. O. Yousef, and K. J. Marians. 1996. DNA strand cleavage is required for replication fork arrest by a frozen topoisomerase-quinolone-DNA ternary complex. J. Biol. Chem. 271:26424-26429.
8. Hori, S., Y. Ohshita, Y. Utsui, and K. Hiramatsu. 1993. Sequential acquisition of norfloxacin and ofloxacin resistance by methicillin-resistant and -susceptible Staphylococcus aureus. Antimicrob. Agents Chemother. 37:2278-2284.
9. Howard, B. M. A., R. J. Pinney, and J. T. Smith. 1993. Function of the SOS process in repair of DNA damage induced by modern 4-quinolones. J. Pharm. Pharmacol. 45:658-662.
10. Isegawa, Y., J. Sheng, Y. Sokawa, K. Yamanishi, O. Nakagomi, and S. Ueda. 1992. Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction (PCR): application to sequencing 6.5 kb genome segment of hantavirus strain B-1. Mol. Cell. Probes 6:467-475.
11. Ito, H., H. Yoshida, M. Bogaki-Shonai, T. Niga, H. Hattori, and S. Nakamura. 1994. Quinolone resistance mutations in the DNA gyrase gyrA and gyrB genes of Staphylococcus aureus. Antimicrob. Agents Chemother. 38: 2014-2023.
12. Kaatz, G. W., S. M. Seo, and C. A. Ruble. 1993. Efflux-mediated fluoroquinolone resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 37:1086-1094.
13. Kornblum, J., B. J. Hartman, R. P. Novick, and A. Tomasz. 1986. Conversion of a homogeneously methicillin-resistant strain of Staphyloccocus aureus to heterogeneous resistance by Tn551-mediated insertional inactivation. Eur. J. Clin. Microbiol. 5:714-718.
14. Kreiswirth, B. N., S. Lofdahl, M. J. Betley, M. O'Reilly, P. M. Schlievert, M. S. Bergdoll, and R. P. Novick. 1983. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature (London) 305:709-712.
15. Lewin, C. S., B. M. A. Howard, N. T. Ratcliffe, and J. T. Smith. 1989. 4-Quinolones and the SOS response. J. Med. Microbiol. 29:139-144.
16. Lloyd, R. G., and C. Buckman. 1991. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. J. Bacteriol. 173:1004-1011.
17. Lloyd, R. G., and G. J. Sharples. 1991. Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12. J. Bacteriol. 173: 6837-6843.
18. Lloyd, R. G., and G. J. Sharples. 1993. Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli. Nucleic Acids Res. 21:1719-1725.
19. Low, B. 1968. Formation of merodiploids in matings with a class of Rec recipient strains of Escherichia coli K-12. Proc. Natl. Acad. Sci. USA 60: 160-167.
20. Martin, B., G. J. Sharples, O. Humbert, R. G. Lloyd, and J.-P. Claverys. 1996. The mmsA locus of Staphylococcus pneumoniae encodes a RecG-like protein involved in DNA repair and in three-strand recombination. Mol. Microbiol. 19:1035-1045.
21. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
22. Ng, E. Y. W., M. Trucksis, and D. C. Hooper. 1994. Quinolone resistance mediated by norA: physiologic characterization and relationship to flqB, a quinolone resistance locus on the Staphylococcus aureus chromosome. Antimicrob. Agents Chemother. 38:1345-1355.
23. Niga, T., and H. Ito. Unpublished data.
24. Nishibuchi, M., K. Kumagai, and J. B. Kaper. 1991. Contribution of the tdh1 gene of Kanagawa phenomenon-positive Vibrio parahaemolyticus to production of extracellular thermostable direct hemolysin. Microb. Pathog. 11:453-460.
25. Novick, R. P. 1991. Genetic systems in staphylococci. Methods Enzymol. 204:587-636.
26. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
27. Shaw, J. H., and D. B. Clewell. 1985. Complete nucleotide sequence of macrolide-lincosamide-streptogramin B-resistance transposon Tn917 in Streptococcus faecalis. J. Bacteriol. 164:782-796.
28. Shen, L. L., W. E. Kohlbrenner, D. Weigl, and J. Baranowski. 1989. Mechanism of quinolone inhibition of DNA gyrase. J. Biol. Chem. 264:2973-2978.
29. Sreedharan, S., M. Oram, B. Jensen, L. R. Peterson, and L. M. Fisher. 1990. DNA gyrase gyrA mutations in ciprofloxacin-resistant strains of Staphylococcus aureus: close similarity with quinolone resistance mutations in Escherichia coli. J. Bacteriol. 172:7260-7262.
30. Sreedharan, S., L. R. Peterson, and L. M. Fisher. 1991. Ciprofloxacin resistance in coagulase-positive and -negative staphylococci: role of mutations at serine 84 in the DNA gyrase A protein of Staphylococcus aureus and Staphylococcus epidermidis. Antimicrob. Agents Chemother. 35:2151-2154.
31. Whitby, M. C., S. D. Vincent, and R. G. Lloyd. 1994. Branch migration of Holliday junctions: identification of RecG protein as a junction specific DNA helicase. EMBO J. 13:5220-5228.
32. Willmott, C. J. R., S. E. Critchlow, I. C. Eperon, and A. Maxwell. 1994. The complex of DNA gyrase and quinolone drugs with DNA forms a barrier to transcription by RNA polymerase. J. Mol. Biol. 242:351-363.
33. Yamagishi, J.-I., T. Kojima, Y. Oyamada, K. Fujimoto, H. Hattori, S. Nakamura, and M. Inoue. 1996. Alterations in the DNA topoisomerase IV grlA gene responsible for quinolone resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 40:1157-1163.
34. Yoshida, H., M. Bogaki, S. Nakamura, K. Ubukata, and M. Konno. 1990. Nucleotide sequence and characterization of the Staphylococcus aureus norA gene, which confers resistance to quinolones. J. Bacteriol. 172:6942-6949.
35. Yoshida, H., M. Nakamura, M. Bogaki, H. Ito, T. Kojima, H. Hattori, and S. Nakamura. 1993. Mechanism of action of quinolones against Escherichia coli DNA gyrase. Antimicrob. Agents Chemother. 37:839-845.