Freeze shattering: A simple and effective method for permeabilizing higher plant cell walls

Journal of Microscopy

Volumn 188, Issue 1, 1997, Pages 51-61

  Wasteneys, G O ^a,b   Willingale Theune, J ^b   Menzel, D ^b  

^a AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^b Max Planck Inst F Zellbiologie   (Germany)

Author keywords

Actin filaments; Allium; Arabidopsis; Confocal laser scanning microscopy; Immunofluorescence; Microtubules; Tradescantia

Indexed keywords

COMMERCE; CONFOCAL MICROSCOPY; FLUORESCENCE; HISTOLOGY; PLANT CELL CULTURE; PLANTS (BOTANY); PROTEINS; SURFACE ANALYSIS; TISSUE;

ACTIN FILAMENT; ALLIUM; ARABIDOPSIS; CONFOCAL LASER SCANNING MICROSCOPY; CONFOCAL-LASER-SCANNING-MICROSCOPY; HIGHER PLANTS; IMMUNOFLUORESCENCE; MICROTUBULES; PLANT CELL WALL; TRADESCANTIUM;

CELLS;

ACTIN; PHALLOIDIN; RHODAMINE; TUBULIN;

ARTICLE; CELL MEMBRANE PERMEABILITY; CELL STRUCTURE; CONFOCAL LASER MICROSCOPY; ENZYME DEGRADATION; FREEZE FRACTURE; PLANT CELL; PRIORITY JOURNAL;

EID: 0030712356     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1977.2390796.x     Document Type: Article

References (35)

1. Baskin, T.I., Busby, C.H., Fowke, L.C., Sammut, M. & Gubler, F. (1992) Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta, 187, 405-413.
2. Baskin, T.I., Miller, D.D., Vos, J.W., Wilson, J.E. & Hepler, P.K. (1996) Cryofixing single cells and muticellular specimens enhances structure and immunocytochemistry for light microscopy. J. Microsc. 182, 149-161.
3. Braun, M. (1996) Immunolocalization of myosin in rhizoids of Chara globularis Thuill. Protoplasma, 191, 1-8.
4. Braun, M. & Sievers, A. (1994) Role of the microtubule cytoskeleton in gravisensing Chara rhizoids. Eur. J. Cell Biol. 63, 289-298.
5. Cleary, A.L. (1995) F-actin redistributions at the division site in living Tradescantia stomatal complexes as revealed by microinjection of rhodamine phalloidin. Protoplasma. 185, 152-165.
6. Cleary, A.L., Gunning, B.E.S., Wasteneys, G.O. & Hepler, P.K. (1992) Microtubule and F-actin dynamics at the division site in living Tradescantia stamen hair cells. J. Cell Sci. 103, 977-988.
7. Craig, S., Goodchild, D.J. & Millerd, A. (1979) Immunofluorescent localization of pea storage proteins in glycol methacrylate embedded tissue. J. Histochem. Cytochem. 27, 1312-1316.
8. Franke, W., Seib, E., Osborn, M., Weber, K., Herth, W. & Falk, H. (1977) Tubulin-containing structures in the anastral mitotic apparatus of endosperm cells of the plant Leucogum aestivum as revealed by immunofluorescence. Cytobiology. 15, 24-48.
9. Graham, T.A., Gunning, B.E.S. (1970) Localisation of legumin and vicilin in bean cotyledon cells using fluorescent antibodies. Nature, 228, 81-82.
10. Gubler, F. (1989) Immunofluorescence localisation of microtubules in plant root tips embedded in butyl-methyl methacrylate. Cell Biol. Int. Rep. 13, 137-145.
11. Gunning, B.E.S. & Wick, S.M. (1985) Preprophase bands, phragmoplasts, and spatial control of cytokinesis. J. Cell Sci. Suppl. 2, 157-179.
12. Hepler, P.K., Cleary, A.I., Gunning, B.E.S., Wadsworth, P., Wasteneys, G.O. & Zhang, D.H. (1993) Cytoskeletal dynamics in living plant cells, Cell Biol. Int. 17, 127-142.
13. Hush, J.M., Wadsworth, P., Callaham, D.A. & Hepler, P.K. (1994) Quantification of microtubule dynamics in living plant cells using fluorescence redistribution after photobleaching. J. Cell Sci. 107, 775-784.
14. Knox, R.B. (1970) Freeze-sectioning of plant tissues. Stain Technol. 45, 265-272.
15. Larsen, P.M. & Wolniak, S.M. (1994) Asynchronous entry into anaphase by okadaic acid: spindle microtubule organization and microtubule/kinetochore attachments. Protoplasma, 177, 53-65.
16. Lee, N., Wetstein, H. & Bornman, C.H. (1989) Cortical microtubule organization in Vitis protoplasts as affected by concentration of enzyme isolation medium and duration of incubation. Physiol. Plant. 77, 27-32.
17. Liebe, S., Quader, H. (1994) Myosin in onion (Allium cepa) bulb scale epidermal cells: involvement of dynamics of organelles and endoplasmic reticulum. Physiol. Plant. 90, 114-124.
18. Lloyd, C.W., Slabas, A.R., Powell, A.J. & Lowe, S.B. (1980) Microtubules, protoplasts and plant cell shape. Planta, 147, 500-506.
19. Marc, J. & Hackett, W.P. (1989) A new method for immunofluorescent localization of microtubules in surface cell layers: application to the shoot apical meristem of Hedera. Protoplasma, 148, 70-79.
20. Menzel, D. & Schliwa, M. (1986) Motility in the siphonous green alga Bryopsis. 1. spatial organization of the cytoskeleton and organelle movement. Eur. J. Cell Biol. 40, 275-285.
21. Panteris, E., Apostolakos, P. & Galatis, B. (1993) Microtubules and morphogenesis in ordinary epidermal cells of Vigna sinensis leaves. Protoplasma, 174, 91-100.
22. Sakaguchi, S., Hogetsu, T. & Hara, N. (1988) Arrangement of cortical microtubules in the shoot apex of Vinca major L. Planta. 175, 403-411.
23. Satiat-Jeunemaitre, B. (1990) Visualization of microtubules in walled plant cells by immunogold silver-enhancement. Cell Biol. Int. Rep 14, 1109-1118.
24. Sawitzky, H. & Grolig, F. (1995) Phragmoplast of the green alga Spirogyra is functionally distinct from the higher plant phragmoplast, J. Cell Biol. 130, 1359-1371.
25. Sonobe, S. & Shibaoka, H. (1989) Cortical line actin filaments in higher plant cells visualized by rhodamine-phalloidin after pretreatment with m-maleimidobenzoyl N-hydroxysuccinimide ester. Protoplasma, 148, 80-86.
26. Tiwari, S.C., Polito, V.S. (1990) The initiation and organization of microtubules in germinating pear (Pyrus communis L.) pollen. Eur J. Cell Biol. 53, 384-389.
27. Tiwari, S.C., Wick, S.M., Williamson, R.E. & Gunning, B.E.S. (1984) Cytoskeleton and integration of cellular function in cells of higher plants. J. Cell Biol. 99, 63s-69s.
28. Traas, J.A., Doonan, J.H., Rawlins, D.J., Shaw, P.J., Watts, J. & Lloyd, C.W. (1987) An actin network is present in the cytoplasm throughout the cell cycle of carrot cells and associates with the dividing nucleus. J. Cell Biol. 105, 387-395.
29. Van der Valk, P., Rennie, P.J., Connolly, J.A. & Fowke, L.C. (1980) Distribution of cortical microtubules in tobacco protoplasts. An immunofluorescence and ultrastructural study. Protoplasma. 105, 27-43.
30. Wasteneys, G.O., Collings, D.A., Gunning, B.E.S., Hepler, P.K., Menzel, D. (1996) Actin in living and fixed characean internodal cells: identification of a cortical array of line actin strands and chloroplast actin rings. Protoplasma, 190, 25-38.
31. Wasteneys, G.O., Gunning, B.E.S. & Hepler, P.K. (1993) Microinjection of fluorescent brain tubulin reveals dynamic properties of cortical microtubules in living plant cells. Cell Motil. Cytoskel. 24, 205-213.
32. Wick, S.M., Seagull, R.W., Osborn, M., Weber, K., Gunning, B.E.S. (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J. Cell Biol. 89, 685-690.
33. Williamson, R.E., Toh, B.H. (1979) Motile models of plant cells and the immunofluorescent localization of actin in a motile Chara cell model. Cell Motility. Molecules and Organization (ed. by S. Hatano. H. Ishikawa and H. Sato). pp. 339-346. Tokyo University Press, Tokyo.
34. Zhang, D., Wadsworth, P., Hepler, P.K. (1990) Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin. Proc. Natl. Acad. Sci. USA, 87, 8820-8824.
35. Zhang, D., Wadsworth, P. & Hepler, P.K. (1993) Dynamics of microfilaments are similar, but distinct from microtubules during cytokinesis in living, dividing plant cells. Cell Motil. Cytoskel. 24, 151-155.