Interneuron migration from basal forebrain to neocortex: Dependence on Dlx genes

Science

Volumn 278, Issue 5337, 1997, Pages 474-476

  Anderson, S A ^a   Eisenstat, D D ^a   Shi, L ^a   Rubenstein, J L R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID; HOMEODOMAIN PROTEIN;

ANIMAL CELL; ARTICLE; BRAIN DEVELOPMENT; CELL MIGRATION; EMBRYO; EMBRYO CELL; GENE EXPRESSION; INTERNEURON; MOUSE; NEOCORTEX; NONHUMAN; PRIORITY JOURNAL; TELENCEPHALON;

ANIMALS; CALCIUM-BINDING PROTEIN, VITAMIN D-DEPENDENT; CELL MOVEMENT; CORPUS STRIATUM; CULTURE TECHNIQUES; DNA-BINDING PROTEINS; GAMMA-AMINOBUTYRIC ACID; GENES, HOMEOBOX; GLUTAMATE DECARBOXYLASE; HOMEODOMAIN PROTEINS; INTERNEURONS; MICE; MUTATION; NEOCORTEX; TELENCEPHALON; TRANSCRIPTION FACTORS;

ANIMALIA;

EID: 0030698872     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.278.5337.474     Document Type: Article

References (33)

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21. To combine cell migration experiments with immunofluorescence, plastic strips (∼200 to 400 μm in diameter) coated with Cell Tracker Dil (Molecular Probes) were placed into the LGE of E12.5 to E14.5 slices. After incubation, fixation, and vibratome resectioning (into sections 40 to 50 μm thick), immunofluorescence was performed with the use of fluorescein-conjugated secondary antibodies (Vector). One or two cells per hemisection (about 20%) were clearly double-labeled with Dil and with either GABA or calbindin. However, because of methodological constraints, we believe that this underestimates the percentage of Dil-labeled cells expressing GABA or calbindin. Although markers for pyramidal neurons appear grossly normal in the Dlx-1/2 mutant neocortex (see text), it is possible that some of the cells migrating from the subcortical telencephalon to the neocortex could be pyramidal neuron precursors.
22. Immunohistochemical methods were performed as described (9). Primary antibody sources and dilutions were as follows: GABA polyclonal, Sigma, 1:5000; GAD polyclonal, Chemicon, 1:2000; calbindin polyclonal, Swiss Antibodies, 1:5000. The DLX-1 polyclonal antibody (diluted 1:20) was generated in our laboratory.
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29. The medial ganglionic eminence may also be a source of cells migrating to the neocortex.
30. These results may explain how transplanted embryonic striatal neurons are able to incorporate and diffferentiate in the neocortex [G. Fishell, Development 121, 803 (1995); O. Brustle, U. Maskos, R. D. McKay, Neuron 15, 1275 (1995)].
31. These results may explain how transplanted embryonic striatal neurons are able to incorporate and diffferentiate in the neocortex [G. Fishell, Development 121, 803 (1995); O. Brustle, U. Maskos, R. D. McKay, Neuron 15, 1275 (1995)].
32. This possibility is supported by the finding that cells sharing the same progenitor can be found in the olfactory bulb and the neocortex [C. Walsh and C. L. Cepko, Nature 362, 632 (1993)].
33. Supported by the National Alliance for Research on Schizophrenia and Depression (S.A. and J.L.R.R.). the Veterans Administration (S.A.), the Medical Research Council of Canada (D.D.E.), the March of Dimes, the John Merck Fund, the Human Frontiers Science Program, and National Institute of Mental Health grants RO1 MH51561 and K02 MH01046 (J.L.R.R.). Care of experimental animals was in accordance with institutional guidelines.