Glutathione-dependent conversion to glyoxylate, a major pathway of dichloroacetate biotransformation in hepatic cytosol from humans and rats, is reduced in dichloroacetate-treated rats

Drug Metabolism and Disposition

Volumn 25, Issue 11, 1997, Pages 1223-1227

  James, M O ^b   Cornett, R ^c   Yan, Z ^a   Henderson, G N ^c   Stacpoole, P W ^c  

^a PFIZER INC   (United States)

^b UNIVERSITY OF FLORIDA HEALTH   (United States)

^c NONE

Author keywords

[No Author keywords available]

Indexed keywords

DICHLOROACETIC ACID; GLYOXYLIC ACID;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOTRANSFORMATION; CONTROLLED STUDY; CYTOSOL; DRUG METABOLISM; DRUG TRANSFORMATION; KINETICS; LIVER METABOLISM; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; BIOTRANSFORMATION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOSOL; DICHLOROACETATE; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUTATHIONE; GLYOXYLATES; HUMANS; KINETICS; LIVER; MALE; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA;

EID: 0030691030     PISSN: 00909556     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. P. W. Stacpoole: The pharmacology of dichloroacetate. Metabolism 38, 1124-1144 (1989).
2. R. J. Bull, I. M. Sanchez, M. A. Nelson, J. L. Larson, and A. J. Lansing: Liver tumor induction in B6C3F1 mice by dichloroacetate and trichloroacetate. Toxicology 63, 341-359 (1990).
3. A. B. DeAngelo, F. B. Daniel, M. M. Most, and G. R. Olsen: The carcinogenicity of dichloroacetic acid in the male F344 rat. Toxicology 114, 207-221 (1996)
4. S. H. Curry, A. Lorenz, P. Chu, M. Limacher, and P. W. Stacpoole: Disposition and pharmacodynamics of dichloroacetate (DCA) and oxalate following oral DCA doses. Biopharm. Drug. Dispos. 12, 375-390 (1991).
5. M. O. James, K. V. M. M. Jayanti, G. N. Henderson, and P. W. Stacpoole: In vivo and in vitro metabolism of [14C]-dichloroacetic acid (DCA) in the male Sprague-Dawley rat: The Toxicologist 15, 261 (1995).
6. R. A. Harris, D. W. Crabb, and R. M. Sans: Studies on the regulation of leucine catabolism. II. Mechanism responsible for dichloroacetate stimulation of leucine oxidation by the liver. Arch. Biochem. Biophys. 190, 8-16 (1978)
7. E. L. C. Lin, J. K. Mattox, and F. B. Daniel: Tissue distribution, excretion and urinary metabolism of dichloroacetic acid in the male Fischer 344 rat. J. Toxicol. Environ. Health 38, 19-32 (1993).
8. J. C. Lipscomb, D. A. Mahle, W. T. Brashear, and H. A. Barton: Dichloroacetic acid: metabolism in cytosol. Drug Metab. Dispos. 25, 1202-1205 (1995).
9. M. O. James and P. J. Little: Modification of benzo(a)pyrene metabolism in hepatic microsomes from untreated and induced rats by imidazole derivatives which inhibit monooxygenase activity and enhance epoxide hydrolase activity. Drug Metab. Dispos. 11, 350-354 (1983).
10. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275 (1951)
11. W. H. Habig, M. J. Pabst, and W. B. Jakoby: Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249, 7130-7139 (1974)
12. G. N. Henderson, Z. Yan, M. O. James, N. Davydova, and P. W. Stacpoole: Kinetics and metabolism of chloral hydrate in children: identification of dichloroacetate as a metabolite. Biochem. Biophys. Res. Commun. 236, 695-698 (1997).
13. Z. Yan, G. N. Henderson, M. O. James, and P. W. Stacpoole: Determination of dichloroacetate and its metabolites in human plasma by gas chromatography-mass spectrometry. J. Chromatogr. (B). (In press).
14. K. Eder: Gas chromatographic analysis of fatty acid methyl esters. J. Chromatogr. 671, 113-131 (1995).
15. D. L. Vander Jagt, L. A. Hunsaker, K. B. Garcia, and R. E. Royer: Isolation and characetization of the multiple glutathione S-transferases from human liver. Evidence for unique heme-binding sites. J. Biol. Chem. 260, 11603-11610 (1985).
16. T. P. M. Akerboom, and H. Sies: Assay of glutathione, glutathione disulfide and glutathione mixed disulfides in biological samples. Meth. Enzymol. 77, 373-382 (1981).
17. E. V. Rowsell, K. Snell, J. A. Carnie, and A. H. Al-Tai: Liver L-alanineglyoxylate and L-serine-pyruvate aminotransferase activities: an apparent association with gluconeogenesis. Biochem. J. 115, 1071-1073 (1969).
18. P. R. Steward and J. R. Quayle: The synergistic decarboxylation of glyoxylate and 2-oxoglutarate by an enzyme system from pig liver mitochondria. Biochem. J. 102, 885-897 (1967).
19. V. Sharma and P. O. Schwille: Oxalate production from glyoxylate by lactate dehydrogenase in vitro: inhibition by reduced glutathione, cysteine, cysteamine. Biochem. Int. 27, 431-438 (1992).