Agrin-deficient myotube retains its acetylcholine receptor aggregation ability when challenged with agrin

Journal of Neurochemistry

Volumn 69, Issue 6, 1997, Pages 2555-2563

  Pun, San ^a   Ng, Yu P ^a   Yang, Jie F ^a   Ip, Nancy Y ^a   Tsim, Karl W K ^a  

^a HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Antisense; Nerve muscle coculture; Neuroblastoma; Neuromuscular junction; Synaptogenesis

Indexed keywords

ACETYLCHOLINESTERASE; AGRIN; CHOLINERGIC RECEPTOR; COMPLEMENTARY DNA;

ANIMAL CELL; ARTICLE; CAPPING PHENOMENON; GENETIC TRANSFECTION; MYOTUBE; NEUROBLASTOMA CELL; NEUROMUSCULAR SYNAPSE; NONHUMAN; PRIORITY JOURNAL; SYNAPTOGENESIS;

ANIMALIA; VERTEBRATA;

EID: 0030689638     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.69062555.x     Document Type: Article

References (36)

1. Bowe M. A. and Fallon J. R. (1995) The role of agrin in synapse formation. Annu. Rev. Neurosci. 18, 443-462.
2. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem 72, 248-254.
3. Busis N. A., Daniels M. P., Bauer H. C., Pudimat P. A., Sonderegger P., Schaffner A. E., and Nirenberg M. (1984) Three cholinergic neuroblastoma hybrid cell lines that form few synapses on myotubes are deficient in acetylcholine receptor aggregation molecules and large dense core vesicles. Brain Res. 324, 201-210.
4. Campagna J. A., Ruegg M. A., and Bixby J. L. (1995) Agrin is a differentiation-inducing "stop signal" for motoneurons in vitro. Neuron 15, 1365-1374.
5. Campanelli J. T., Hoch W., Rupp F., Kreiner T., and Scheller R. H. (1991) Agrin mediates cell contact-induced acetylcholine receptor clustering. Cell 67, 909-916.
6. Chomczynski P. and Sacchi N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-choroform extraction. Anal. Biochem. 162, 156-159.
7. Christian C. N., Daniels M. P., Sugiyama H., Vogel Z., Jacques L., and Nelson P. G. (1978) A factor from neurons increases the number of acetylcholine receptor aggregates on cultured muscle cells. Proc. Natl. Acad. Sci. USA 75, 4011-4015.
8. Cohen M. W. and Godfrey E. W. (1992) Early appearance of and neuronal contribution to agrin-like molecules at embryonic frog nerve-muscle synapses formed in culture. J. Neurosci. 12, 2982-2992.
9. Fallon J. R. and Gelfman C. E. (1989) Agrin related molecules are concentrated at acetylcholine receptor cluster in normal and aneural developing muscle. J. Cell Biol. 108, 1527-1535.
10. Ferns M., Hock W., Campanelli J. T., Rupp F., Hall Z. W., and Scheller R. H. (1992) RNA splicing regulates agrin-mediated acetylcholine receptor clustering activity on cultured myotubes. Neuron 8, 1079-1086.
11. Ferns M., Campanelli J. T., Hoch W., Scheller R. H., and Hall Z. W. (1993) The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11, 491-502.
12. Gautam M., Noakes P. G., Moscoso L., Rupp F., Scheller R. H., Merlie J. P., and Sanes J. R. (1996) Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell 85, 525-535.
13. Gesemann M., Denzer A. J., and Ruegg M. A. (1995) Acetycholine receptor-aggregating activity of agrin isoforms and mapping of the active site. J. Cell Biol. 128, 625-636.
14. Godfrey E. W. (1991) Comparison of agrin-like proteins from the extracellular matrix of chicken kidney and muscle with neural asrin, a synapse organizing protein. Exp. Cell Res. 195, 99-109.
15. Godfrey E. W., Dietz M. E., Morstad A. L., Wallskog P. A., and Yorde D. E. (1988a) Acetylcholine receptor-aggregating proteins are associated with the extracellular matrix of many tissues in Torpedo. J. Cell Biol. 106, 1263-1272.
16. Godfrey E. W., Siebenlist R. E., Wallskog P. A., Walters L. M., Bolender D. L., and Yorde D. E. (1988b) Basal lamina components are concentrated in premuscle masses and at early acetylcholine receptor clusters in chick embryo hindlimb muscles. Dev. Biol. 130, 471-486.
17. Hall Z. W. and Sanes J. R. (1993) Synaptic structure and development: the neuromuscular junction. Cell 72 Neuron 10 (Suppl.), 99-121.
18. Hall Z. W. and Sanes J. R. (1993) Synaptic structure and development: the neuromuscular junction. Cell 72 lNeuron 10 (Suppl.), 99-121.
19. Kröger S. and Mann S. (1996) Biochemical and functional characterization of basal lamina-bound agrin in the chick central nervous system. Eur. J. Neurosci. 8, 500-509.
20. Lieth E., Cardasis C. A., and Fallon J. R. (1992) Muscle-derived agrin in cultured myotubes: expression in the basal lamina and at induced acetylcholine receptor clusters. Dev. Biol. 149, 41-54.
21. Magill-Solc C. and McMahan U. J. (1988) Motor neurons contain agrin-like molecules. J. Cell Biol. 107, 1825-1833.
22. McMahan U. J. (1990) The agrin hypothesis. Cold Spring Harb. Symp. Quant. Biol. 55, 407-418.
23. Nitkin R. M., Smith M. A., Magill C., Fallon J. R., Yao M. M., Wallace B. G., and McMahan U. J. (1987) Identification of agrin, a synaptic organizing protein from Torpedo electric organ. J. Cell Biol. 105, 2471-2478.
24. Pun S. and Tsim K. W. K. (1995) Truncated form of pro-acetylcholine receptor-inducing activity (ARIA) induces AChR α-subunit but not AChE transcripts in cultured chick myotubes. Neurosci. Lett. 198, 107-110.
25. Pun S., So K. L., and Tsim K. W. K. ( 1995) Agrin from NG108-15 induces acetylcholine receptor aggregation on primary rat myotube cultures. Soc. Neurosci. Abstr. 21, 1776.
26. Reist N. E., Magill C., and McMahan U. J. (1987) Agrin-like molecules at synaptic sites in normal, denervated, and damaged skeletal muscles. J. Cell Biol. 105, 2457-2469.
27. Reist N. E., Werle M. J., and McMahan U. J. (1992) Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions. Neuron 8, 865-868.
28. Ruegg M. A., Tsim K. W. K., Horton S. E., Kröger S., Escher G., Gensch E. M., and McMahan U. J. (1992) The agrin gene codes for a family of basal lamina proteins that differ in function and distribution. Neuron 8, 691-699.
29. Rupp F., Payan D. G., Magill-Solc C., Cowan D. M., and Scheller R. H. (1991) Structure and expression of a rat agrin. Neuron 6, 811-823.
30. Sambrook J., Fritsch E. F., and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edit. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
31. Smith M. A., Magill-Solc C., Rupp F., Yao Y.-M. M., Schilling J., Snow P., and McMahan U. J. (1992) Isolation and characterization of a cDNA that encodes an agrin homolog in the marine ray. Mol. Cell. Neurosci. 3, 406-417.
32. So K. L., Pun S., Wan D. C. C., and Tsim K. W. K. (1996) Cerebellar granule cells express a specific isoform of agrin that lacks the acetylcholine receptor aggregating activity. FEBS Lett. 379, 63-68.
33. Tsim K. W. K., Ruegg M. A., Escher G., Kröger S., and McMahan U. J. (1992) cDNA that encodes active agrin. Neuron 8, 677-689.
34. Tsim K. W. K., Choi R. C. Y., Dong T. T. X., and Wan D. C. C. (1997) A globular, not asymmetric, form of AChE is expressed in chick motor neurons: down-regulation towards maturity and after denervation. J. Neurochem. 68, 479-487.
35. Wallace B. G. (1986) Aggregating factor from Torpedo electric organ induces patches containing acetylcholine receptors, acetylcholinesterase and butyrylcholinesterase on cultured myotubes. J. Cell Biol. 102, 783-794.
36. Yaffe A. and Saxel O. (1977) Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270, 725-727.