Gradual phenotypic conversion associated with immortalization of cultured human mammary epithelial cells

Molecular Biology of the Cell

Volumn 8, Issue 12, 1997, Pages 2391-2405

  Stampfer, Martha R ^a   Bodnar, Andrea ^b   Garbe, James ^a   Wong, Michelle ^a   Pan, Alison ^a   Villeponteau, Bryant ^b   Yaswen, Paul ^a  

^a LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^b GERON CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TELOMERASE; TRANSFORMING GROWTH FACTOR BETA;

ARTICLE; BREAST EPITHELIUM; CELL GROWTH; CELL IMMORTALIZATION; CELL PROLIFERATION; CELL TRANSFORMATION; DNA REPLICATION; ENZYME ACTIVITY; EPITHELIUM CELL; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; PHENOTYPE; PRIORITY JOURNAL; TELOMERE;

EID: 0030668750     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.12.2391     Document Type: Article

References (15)

1. Rogalla, P., Kazmierczak, B., Rohen, C., Trams, G., Bartnitzke, S., and Bullerdiek, J. (1994). Two human breast cancer cell lines showing decreasing telomeric repeat length during early in vitro passaging. Cancer Genet. Cytogenet. 77, 19-25.
2. Sandhu, C., Garbe, J., Bhattacharya, N., Daksis, J.I., Pan, C.-H., Yaswen, P., Koh, J., Slingerland, J.M. and Stampfer, M.R. (1997). TGF-β stabilizes p15INK4B protein, increases p15INK4B/cdk4 complexes and inhibits cyclin D1/cdk4 association in human mammary epithelial cells. Mol. Cell Biol. 17, 2458-2467.
3. Shay, J.W., Wright, W.E., and Werbin, H. (1993). Toward a molecular understanding of human breast cancer: a hypothesis. Breast Cancer Res. Treat. 25, 83-94.
4. Slingerland, J.M., Hengst, L., Pan, C-H., Alexander, D., Stampfer, M.R., and Reed, S.I. (1994). A novel inhibitor of cyclin/cdk activity detected in TGF-β arrested cells. Mol. Cell Biol. 14, 3683-3694.
5. Smith, J.R., and Pereira-Smith, O.M. (1996). Replicative senescence: implications for in vivo aging and tumor suppression. Science 273, 63-67.
6. Stampfer, M.R. (1982). Cholera toxin stimulation of human mammary epithelial cells in culture. In Vitro 18, 531-537.
7. Stampfer, M.R. (1985). Isolation and growth of human mammary epithelial cells. J. Tissue Culture Methods 9, 107-116.
8. Stampfer, M.R., and Bartley, J.C. (1985). Induction of transformation and continuous cell lines from normal human mammary epithelial cells after exposure to benzo(a)pyrene. Proc. Natl. Acad. Sci. USA 82, 2394-2398.
9. Stampfer, M.R., and Bartley, J.C. (1988). Human mammary epithelial cells in culture: differentiation and transformation. In: Breast Cancer: Cellular and Molecular Biology, ed. R. Dickson, and M. Lippman, Norwall, MA: Kluwer Academic Publishers, 1-24.
10. Stampfer, M.R., Pan, C.H., Hosoda, J., Bartholomew, J., Mendelsohn, J., and Yaswen, P. (1993a). Blockage of EGF receptor signal transduction causes reversible arrest of normal and transformed human mammary epithelial cells with synchronous reentry into the cell cycle. Exp. Cell Res. 208, 175-188.
11. Stampfer, M.R., Yaswen, P., Alhadeff, M., and Hosoda, J. (1993b). TGFβ induction of extracellular matrix associated proteins in normal and transformed human mammary epithelial cells in culture is independent of growth effects. J. Cell Physiol. 155, 210-221.
12. van Steensel, B., and de Lange, T. (1997). Control of telomere length by the human telomeric protein TRF1. Nature 385, 740-743.
13. Walen, K., and Stampfer, M.R. (1989). Chromosome analyses of human mammary epithelial cells at stages of chemically-induced transformation progression to immortality. Cancer Genet. Cytogenet. 37, 249-261.
14. Wright, W.E. and Shay, J.W. (1995). Time, telomeres and tumors: is cellular senescence more than an anticancer mechanism. Trends Cell Biol. 5, 293-297.
15. Wright, W.E., Shay, J.W., and Piatyszek, M.A. (1995). Modulations of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity. Nucleic Acids Res. 23, 3794-3795.