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Volumn 23, Issue 5, 1997, Pages 822-826

High-efficiency T-vector cloning of PCR products by forced A tagging and post-ligation restriction enzyme digestion

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE; DNA; DNA POLYMERASE; DOUBLE STRANDED DNA; RESTRICTION ENDONUCLEASE; RNA;

EID: 0030667920     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97235bm12     Document Type: Article
Times cited : (12)

References (13)
  • 1
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    • Cease, K.B. and C.J. Lohff. 1993. A vector for facile PCR product cloning and modification generating any desired 4-base 5′ overhang: pRPM. BioTechniques 14:250-255.
    • (1993) BioTechniques , vol.14 , pp. 250-255
    • Cease, K.B.1    Lohff, C.J.2
  • 2
    • 0023734473 scopus 로고
    • Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases
    • Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 16:9677-9686.
    • (1988) Nucleic Acids Res. , vol.16 , pp. 9677-9686
    • Clark, J.M.1
  • 3
    • 0028240187 scopus 로고
    • Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments
    • Costa, G.L. and M.P. Weiner. 1994. Polishing with T4 or Pfu polymerase increases the efficiency of cloning of PCR fragments. Nucleic Acids Res. 22:2423.
    • (1994) Nucleic Acids Res. , vol.22 , pp. 2423
    • Costa, G.L.1    Weiner, M.P.2
  • 4
    • 0028010854 scopus 로고
    • Direct cloning of polymerase chain reaction products in an XcmI T-vector
    • Harrison, J., P.L. Molley and S.J. Clark. 1994. Direct cloning of polymerase chain reaction products in an XcmI T-vector. Anal. Biochem. 216:235-236.
    • (1994) Anal. Biochem. , vol.216 , pp. 235-236
    • Harrison, J.1    Molley, P.L.2    Clark, S.J.3
  • 5
    • 0028858881 scopus 로고
    • Cloning PCR products using T-vectors
    • Hengen, P.N. 1995. Cloning PCR products using T-vectors. Trends Biotechnol. 20:85-86.
    • (1995) Trends Biotechnol. , vol.20 , pp. 85-86
    • Hengen, P.N.1
  • 6
    • 0027729359 scopus 로고
    • DNA polymerase-catalyzed addition of non-template extra nucleotides to the 3′ end of a DNA fragment
    • Hu, G. 1993. DNA polymerase-catalyzed addition of non-template extra nucleotides to the 3′ end of a DNA fragment. DNA Cell Biol. 12:763-770.
    • (1993) DNA Cell Biol. , vol.12 , pp. 763-770
    • Hu, G.1
  • 7
    • 0029791306 scopus 로고    scopus 로고
    • A positive screen for cloning PCR products
    • Keese, P. and L. Graf. 1996. A positive screen for cloning PCR products. Nucleic Acids Res. 24:3474-3475.
    • (1996) Nucleic Acids Res. , vol.24 , pp. 3474-3475
    • Keese, P.1    Graf, L.2
  • 8
    • 0028363433 scopus 로고
    • A simple ligation step improves the efficiency of T-overhang vectors
    • Khan, I.M., P.S. Zammit, M. Green and L. Buluwela. 1994. A simple ligation step improves the efficiency of T-overhang vectors. Trends Genet. 10:225-226.
    • (1994) Trends Genet. , vol.10 , pp. 225-226
    • Khan, I.M.1    Zammit, P.S.2    Green, M.3    Buluwela, L.4
  • 9
    • 0025982062 scopus 로고
    • Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products
    • Marchuk, D., M. Drumm, A. Saulino and F.S. Collins. 1991. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 19:1154.
    • (1991) Nucleic Acids Res. , vol.19 , pp. 1154
    • Marchuk, D.1    Drumm, M.2    Saulino, A.3    Collins, F.S.4
  • 10
    • 0024892209 scopus 로고
    • Transcription of infectious Yellow Fever RNA from full-length cDNA templates produced by in vitro ligation
    • Rice, C.M., A. Grakoui, R. Galler and T.J. Chambers. 1989. Transcription of infectious Yellow Fever RNA from full-length cDNA templates produced by in vitro ligation. New Biol. 1:285-296.
    • (1989) New Biol. , vol.1 , pp. 285-296
    • Rice, C.M.1    Grakoui, A.2    Galler, R.3    Chambers, T.J.4
  • 12
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    • Optimized conditions for cloning PCR products into an XcmI T-vector
    • Schutte, B.C., K. Ranade, J. Pruessner and N. Dracopoli. 1997. Optimized conditions for cloning PCR products into an XcmI T-vector. BioTechniques 22:40-44.
    • (1997) BioTechniques , vol.22 , pp. 40-44
    • Schutte, B.C.1    Ranade, K.2    Pruessner, J.3    Dracopoli, N.4
  • 13
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    • Direct cloning of unmodified PCR products by exploiting an engineered restriction site
    • Testori, A., I. Listowsky and P. Sollitti. 1994. Direct cloning of unmodified PCR products by exploiting an engineered restriction site. Gene 143:151-152.
    • (1994) Gene , vol.143 , pp. 151-152
    • Testori, A.1    Listowsky, I.2    Sollitti, P.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.