Optimized conditions for cloning PCR products into an XcmI T-vector

BioTechniques

Volumn 22, Issue 1, 1997, Pages 40-44

  Schutte, Brian C ^a,d   Ranade, Koustubh ^b   Pruessner, Jonathan ^a   Dracopoli, Nic ^c  

^a UNIVERSITY OF IOWA   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c CELERA   (United States)

^d UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; FUNGAL DNA;

ARTICLE; BACTERIUM COLONY; DNA SEQUENCE; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; LIGATION; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0031023680     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97221bm06     Document Type: Article

References (9)

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2. Cha, J., W. Bishai and S. Chandrasegaran. 1993. New vectors for direct cloning of PCR products [published erratum appears in Gene 1994; 141:149]. Gene 136:369-370.
3. Clark, J.M. 1988. Novel non-templated nucleotide addition reactions catalyzed by prokaryotic and eukaryotic DNA poymerases. Nucleic Acids Res. 16:9677-9686.
4. Harrison, J., P.L. Molloy and S.J. Clark. 1994. Direct cloning of polymerase chain reaction products in an XcmI T-vector. Anal. Biochem. 216:235-236.
5. Ichihara Y. and Y. Kurosawa. 1993. Construction of new T-vectors for direct cloning of PCR products. Gene 130:153-154.
6. Kovalic, D., J.H. Kwak and B. Weisblum. 1991. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19:4560.
7. Mead, D.A., N.K. Pey, C. Herrnstadt, R.A. Marcil and L.M. Smith. 1991. A universal method for the direct cloning of PCR amplified nucleic acid. Bio/Technology 9:657-663.
8. Mertz, L.M. and A. Rashtchian. 1994. PCR radioactive labeling system: a rapid method for synthesis of high specific activity DNA probes. Focus 16:45-48.
9. Testori, A., I. Listowsky and P. Sollitti. 1994. Direct cloning of unmodified PCR products by exploiting an engineered restriction site. Gene 143:151-152.